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The map label for this gene is thyA [H]

Identifier: 117921478

GI number: 117921478

Start: 3643150

End: 3643944

Strand: Reverse

Name: thyA [H]

Synonym: Shewana3_3039

Alternate gene names: 117921478

Gene position: 3643944-3643150 (Counterclockwise)

Preceding gene: 117921479

Following gene: 117921477

Centisome position: 73.29

GC content: 47.3

Gene sequence:

>795_bases
ATGCAACAGTATTTAGACTTGATGAAGCACATCTTAGCCGAAGGTGTGGATAAATCAGACCGCACAGGTACCGGCACTCG
CTCAGTGTTTGGTTATCAAATGCGTTTCGATTTGAGTAAGGGCTTTCCCTTAGTCACTACCAAGAAGTGCCATATGCGCT
CGATTATTCATGAGCTATTGTGGTTCCTGAAAGGCGATACCAACATTGCTTATCTGCGTGAGAATAAAGTCAGTATTTGG
GACGAATGGGCCGATGAAAACGGCGATTTAGGCCCTGTTTATGGTGCGCAGTGGCGCAGCTGGCCGACCCAAAGTGGCGA
TGCTATCGACCAAATCGCTCAGGTAATTGCACAAATCAAATCTCAACCTGATTCACGTCGTTTAATTGTTTCGGCGTGGA
ACGTGGGTGAACTGGATAAAATGGCGCTGGCACCTTGCCATGCCTTTTTCCAATTCTATGTTGCCGATGGCAAATTATCC
TGTCAGCTGTATCAGCGTAGCTGTGATGTGTTCTTAGGCTTACCTTTCAATATCGCCAGTTATGCGCTGCTGACCATGAT
GGTGGCACAGCAATGTGATTTAGCTTTGGGTGACTTTGTGTGGACGGGCGGCGATACCCATTTGTACTCCAACCATATGG
AACAAACGGCGTTGCAGTTAAGCCGTGAGCCAAGACAGTTACCCACCATGACAATCCTGCGTAAACCCGCTTCAATCTTT
GATTATCAATTCGAAGATTTCGAGCTAACTCACTACGATCCACACCCGCATATCAAAGCCCCTGTCGCCGTTTAA

Upstream 100 bases:

>100_bases
TAGGTCTCTACTGGGGCTTTATGACGATGGGGCAGATCCTCTCTGTGCCGATGATCCTCTTCGGTTTATATTTAATTCTT
CGTCCAGAGGGTAAGCAGTA

Downstream 100 bases:

>100_bases
TGGCGAATTAATCGACTAACACCTCGGAATTATCGAGGTGTTTTGTCGAAACTATCGATTATACTTGTTTTTTTTCTTCG
TCTACACTCAGTTCATTATG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MQQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVTTKKCHMRSIIHELLWFLKGDTNIAYLRENKVSIW
DEWADENGDLGPVYGAQWRSWPTQSGDAIDQIAQVIAQIKSQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPRQLPTMTILRKPASIF
DYQFEDFELTHYDPHPHIKAPVAV

Sequences:

>Translated_264_residues
MQQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVTTKKCHMRSIIHELLWFLKGDTNIAYLRENKVSIW
DEWADENGDLGPVYGAQWRSWPTQSGDAIDQIAQVIAQIKSQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPRQLPTMTILRKPASIF
DYQFEDFELTHYDPHPHIKAPVAV
>Mature_264_residues
MQQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVTTKKCHMRSIIHELLWFLKGDTNIAYLRENKVSIW
DEWADENGDLGPVYGAQWRSWPTQSGDAIDQIAQVIAQIKSQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPRQLPTMTILRKPASIF
DYQFEDFELTHYDPHPHIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=51.063829787234, Blast_Score=280, Evalue=7e-76,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=79.5454545454545, Blast_Score=456, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=47.3498233215548, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=39.7260273972603, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=50.7042253521127, Blast_Score=280, Evalue=9e-76,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30130; Mature: 30130

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVTTKKCHMRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLKGDTNIAYLRENKVSIWDEWADENGDLGPVYGAQWRSWPTQSGDAIDQIAQVIAQIK
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEEEECCHHHHH
YALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPRQLPTMTILRKPASIF
HHHHHHHHHHHCCCHHHCEEEECCCCHHHHHHHHHHHHHHHCCCHHCCHHHHHHCCHHHH
DYQFEDFELTHYDPHPHIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MQQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVTTKKCHMRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLKGDTNIAYLRENKVSIWDEWADENGDLGPVYGAQWRSWPTQSGDAIDQIAQVIAQIK
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEEEECCHHHHH
YALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPRQLPTMTILRKPASIF
HHHHHHHHHHHCCCHHHCEEEECCCCHHHHHHHHHHHHHHHCCCHHCCHHHHHHCCHHHH
DYQFEDFELTHYDPHPHIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA