Click here to switch to the map view.

The map label for this gene is nadB [H]

Identifier: 117921472

GI number: 117921472

Start: 3638013

End: 3639626

Strand: Direct

Name: nadB [H]

Synonym: Shewana3_3033

Alternate gene names: 117921472

Gene position: 3638013-3639626 (Clockwise)

Preceding gene: 117921459

Following gene: 117921483

Centisome position: 73.17

GC content: 51.49

Gene sequence:

>1614_bases
ATGAAACAAGTAGTTGAACACCAATCTGACATATTAGTCATAGGTAGCGGTGCCGCAGGTCTGACACTAGCTTTGCATCT
TGCTGAAAAAGCAAACGTAATTCTGCTCTCAAAAGGCCCACTCTCGGAAGGGTCGACATACTACGCCCAAGGCGGTATCG
CCTCCGTGTTCGATGAAAGTGATACCATCGAATCCCATGTGGCCGATACGTTAGTGGCTGGCGCCGGCTTGTGCGATAAA
GAAGTGGTAACTTTTACCGCTGAAAACGCCAAGAGCGCCATGCAATGGTTAATTGAATGTGGTGTGGCATTCGATAAAGA
AGAAACCACTGGCGATAATGCCAAAGACGCGCCTTATCACTTGACCCGCGAAGGCGGCCACAGCCATAGACGCATTCTGC
ATGCCGCCGATGCGACTGGCAAAGAAGTGCAAACCACACTGCAAGAACGCGCCCTCGCCCATCCCAATATTCAAGTTTTA
GAACGTTACAACGCCATCGACCTCATCACCACCCGTAAATTAAATCGCCCTGGCAATCGCGTGTTAGGCGCTTATGTGTG
GAACCGTAATGCCGAACAGGTTGAAACCATTAAGGCAAAATTTGTCGCATTAGCCACGGGCGGTAGCTCTAAGGTCTACC
AATACACCTCAAACCCCGATGTGGCGAGTGGTGATGGTATTGCCATGGCGTGGCGTGCAGGTTGCCGTGTGGCTAACATG
GAGTTTAATCAATTCCATCCGACCTGCCTTTACCATGCCGATGCGCGTAACTTCCTGCTGACCGAAGCCCTGCGCGGTGA
AGGGGCTTATTTACGCCGCCCCGATGGCAGCCGCTTTATGCCAGACTTTGACGAGCGAGCCGAACTCGCGCCTCGCGATA
TCGTAGCCCGCGCCATCGACTATGAGATGAAGCGTTTAGGTGCCGATTGCGTCTACTTAGACATTAGCCATAAAGACAGC
GACTTTATCATCAAGCACTTCCCAACGATCTATAGTCGCTGCTTGGAACTGGGTATCGATATCACCAAGGATGCCATTCC
AGTGGTGCCTGCCGCCCACTATACCTGTGGTGGCGTGATGACGGATTTGCACGGTCAAACCGACCTCAATGGTCTCTATG
CCATCGGCGAAGTAGCCTATACCGGCTTACATGGTGCGAACCGCTTGGCGAGTAACTCGCTACTCGAGTGTTTGGTATTT
GCCCGCGCTGCGTCGCAGGATATTGAGAGCCAAATGCACAAAATTCCATTACCGGGGCAAATTCCGGCGTGGGACGAGAG
TAAAGTTTCCAACTCAGATGAGGAAGTGGTAATTGCCCACAACTGGCACGAACTGCGCCTCTTTATGTGGGACTATGTGG
GGATCGTCCGCTCAGATAAACGTTTGGAGCGCGCACTGCGTCGCTGCTTGATGCTGCAACAGGAAATCCAAGAGTATTAC
AGCAACTTCAGAGTCAGCAATAACCTACTGGAACTGCGTAACTTAGTGCAAGTGGCGGAGCTGATTATCCGCTGCGCTAT
GGATCGTAAAGAGAGCCGTGGTCTGCACTATAATATCGACCATCCCGAGAAGGTAGACTCTCCACAACCAACGATTCTGC
AACCTGAAAAATAA

Upstream 100 bases:

>100_bases
TCTAAGAATTATACAAAAAAAGTAAGGTTAAAAGGAAAGTACCCATAATCGGTGAGTTGGTTTACCATAGATGGCACTCT
CAGTCATAGCATGATACCCG

Downstream 100 bases:

>100_bases
CCGAGTTGCTTAAGGGACATTTTTGACGGGCTAAATCTACTCAGATTTAGCCCGTTTTATTTAGGCTGAAGGAACCATTT
TAGATATGAATTAAAGGGAC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKQVVEHQSDILVIGSGAAGLTLALHLAEKANVILLSKGPLSEGSTYYAQGGIASVFDESDTIESHVADTLVAGAGLCDK
EVVTFTAENAKSAMQWLIECGVAFDKEETTGDNAKDAPYHLTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVL
ERYNAIDLITTRKLNRPGNRVLGAYVWNRNAEQVETIKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGADCVYLDISHKDS
DFIIKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAASQDIESQMHKIPLPGQIPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMDRKESRGLHYNIDHPEKVDSPQPTILQPEK

Sequences:

>Translated_537_residues
MKQVVEHQSDILVIGSGAAGLTLALHLAEKANVILLSKGPLSEGSTYYAQGGIASVFDESDTIESHVADTLVAGAGLCDK
EVVTFTAENAKSAMQWLIECGVAFDKEETTGDNAKDAPYHLTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVL
ERYNAIDLITTRKLNRPGNRVLGAYVWNRNAEQVETIKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGADCVYLDISHKDS
DFIIKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAASQDIESQMHKIPLPGQIPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMDRKESRGLHYNIDHPEKVDSPQPTILQPEK
>Mature_537_residues
MKQVVEHQSDILVIGSGAAGLTLALHLAEKANVILLSKGPLSEGSTYYAQGGIASVFDESDTIESHVADTLVAGAGLCDK
EVVTFTAENAKSAMQWLIECGVAFDKEETTGDNAKDAPYHLTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVL
ERYNAIDLITTRKLNRPGNRVLGAYVWNRNAEQVETIKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGADCVYLDISHKDS
DFIIKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAASQDIESQMHKIPLPGQIPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMDRKESRGLHYNIDHPEKVDSPQPTILQPEK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=559, Percent_Identity=31.8425760286225, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI1788928, Length=537, Percent_Identity=70.9497206703911, Blast_Score=805, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=530, Percent_Identity=30.5660377358491, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI1786942, Length=562, Percent_Identity=30.4270462633452, Blast_Score=182, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17505833, Length=521, Percent_Identity=29.3666026871401, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17550100, Length=574, Percent_Identity=30.8362369337979, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI71986328, Length=464, Percent_Identity=27.5862068965517, Blast_Score=104, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322701, Length=561, Percent_Identity=31.0160427807487, Blast_Score=206, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6322416, Length=570, Percent_Identity=30, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322511, Length=487, Percent_Identity=26.2833675564682, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320788, Length=487, Percent_Identity=26.2833675564682, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24663005, Length=565, Percent_Identity=29.9115044247788, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17137288, Length=562, Percent_Identity=30.9608540925267, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24655642, Length=562, Percent_Identity=30.9608540925267, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24655647, Length=562, Percent_Identity=30.9608540925267, Blast_Score=193, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59746; Mature: 59746

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVVEHQSDILVIGSGAAGLTLALHLAEKANVILLSKGPLSEGSTYYAQGGIASVFDES
CCCHHCCCCCEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCHHHCCCC
DTIESHVADTLVAGAGLCDKEVVTFTAENAKSAMQWLIECGVAFDKEETTGDNAKDAPYH
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVLERYNAIDLITTRKLNRPGNR
EECCCCCHHHHEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEHHCCCCCCCE
VLGAYVWNRNAEQVETIKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EEEEEEECCCHHHHHHHHEEEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEECC
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHH
YEMKRLGADCVYLDISHKDSDFIIKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
TDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAASQDIESQMHKIPLPGQ
CCCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMDRKESRGLHYNIDHPEKVDSPQPTILQPEK
HHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCEECCCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKQVVEHQSDILVIGSGAAGLTLALHLAEKANVILLSKGPLSEGSTYYAQGGIASVFDES
CCCHHCCCCCEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCHHHCCCC
DTIESHVADTLVAGAGLCDKEVVTFTAENAKSAMQWLIECGVAFDKEETTGDNAKDAPYH
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVLERYNAIDLITTRKLNRPGNR
EECCCCCHHHHEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEHHCCCCCCCE
VLGAYVWNRNAEQVETIKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EEEEEEECCCHHHHHHHHEEEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEECC
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHH
YEMKRLGADCVYLDISHKDSDFIIKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
TDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAASQDIESQMHKIPLPGQ
CCCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMDRKESRGLHYNIDHPEKVDSPQPTILQPEK
HHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCEECCCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]