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The map label for this gene is lepA [H]

Identifier: 117921467

GI number: 117921467

Start: 3633207

End: 3634997

Strand: Reverse

Name: lepA [H]

Synonym: Shewana3_3028

Alternate gene names: 117921467

Gene position: 3634997-3633207 (Counterclockwise)

Preceding gene: 117921468

Following gene: 117921466

Centisome position: 73.11

GC content: 47.68

Gene sequence:

>1791_bases
ATGAAACACATTAGAAACTTCTCAATTATTGCCCATATCGACCATGGTAAATCGACGCTATCAGATCGTCTTATTCAGGT
TTGTGGCGGGTTAAGCGACCGTGAAATGGATGCGCAAGTTCTTGATTCTATGGATCTAGAGCGTGAGCGCGGTATCACGA
TTAAGGCCCAGAGCGTCACGTTGGAATACAAAGCCAAAAATGGCGAGACTTACCAACTTAACTTTATTGATACTCCAGGC
CACGTTGACTTTTCCTATGAAGTATCTCGTTCATTAGCCGCCTGTGAGGGTGCGCTGCTCGTGGTGGATGCGGGTCAAGG
TGTTGAAGCCCAGACGCTTGCAAACTGTTACACCGCGCTGGATATGAATCTGGACGTAGTACCTATCCTGAACAAAATCG
ACTTACCCCAAGCCGATCCTGAGCGCGTAGCCGCTGAGATTGAAGACATCGTGGGTATCGATGCGATGAACGCTGTGCGT
TGCTCGGCCAAAACCGGTGTAGGTATTGACGAGGTATTAGAAGTCATCGTCGATCAAATTCCACCGCCAGAAGGCGACCC
AGAGGCGCCGCTACAAGCGTTGATCATCGACTCTTGGTTTGACAGCTACTTAGGCGTTGTTTCTCTCGTACGTATTAAAA
ATGGCGTGCTGAAAAAAGGCGACAAGTTTAAGGTAATGTCTACCGGACAAAACCACACCGCAGATCGCGTGGGTATTTTC
ACGCCTAAACAAACCGATAAAACCGAGCTGAAAACCGGTGAAGTAGGTTTCGTTATCGCGGGTATTAAAGAGATTCATGG
TGCACCAGTGGGTGATACCTTGACGCTGGCTAAGCATGGTGCTGAAAAGCCATTACCCGGCTTTAAGAAAGTGAAGCCTC
AGGTATACGCGGGTGTGTTCCCGATTTCGACCGATGAATATGAAAACTTCCGCGATGCGCTGAACAAACTCAGCCTCAAC
GATGCGTCATTGTTCTTCGAGCCTGAAAGTTCATCAGCCCTAGGTTTTGGTTTCCGTATTGGCTATTTAGGCCTGCTCCA
CATGGAAATCATTCAAGAGCGTTTAGAGCGTGAATACGATCTTGACCTTATCACGACGGCGCCTACCGTAGTGTATGAGG
TGCTGTTGACCAGTGGTGAAACCATCTATGTTGATAACCCAGCGGACTTGCCGGCGATTAACAACATCGAAGAGATGCGT
GAGCCAATCGTTGAAGCTAACATTCTTGTGCCAAAAGAATACTTAGGTAACGTGATTACCCTGTGTATCGAAAAACGCGG
TACCCAAGTGAACATGGTTTACCACGGCAACCAAGTGGCCGTGACTTACCATCTGCCGATGGCAGAAGTGGTAATGGACT
TCTTCGACCGCTTAAAATCAACCAGCCGTGGTTATGCATCGTTAGAATACAACTTCATCCGCTTCGATCCTGCGGACATG
GTGCGCTTAGATATCTTAATCAACGGCGACCGCGTAGACGCATTGGCGATGATTATTCACCGTTCAAACATTCGTCACCG
TGGTTTGGCGTTGGTTGAGAAGATGAAAGAGCTTATCCCTCGTCAGATGTTTGATATTGCGATTCAAGCCGCTGTTGGCA
GCCAAATTATTGCCCGTTCTACCGTAAAAGCCTTACGTAAAGACGTAACGGCTAAGTGTTACGGTGGTGACGTTTCCCGT
AAGAAGAAACTCTTGAATAAACAAAAAGAGGGTAAGAAACGGATGAAACAAGTGGGTAACGTTGAGGTGCCGCAAGAGGC
GTTCTTAGCGGTACTTAAGCTAAACGAGTAA

Upstream 100 bases:

>100_bases
GAGAAGGATGAGTGAATCTGATTGGTATTGAAACCGCAATCAGGTACAATTTCGCACCTTTGAACCGTATCCATTTTTCA
GTATTAGTCATAGCAGCATA

Downstream 100 bases:

>100_bases
CACTCGAGATAACACAATTTAGCTATTATTGCGCCGCAGTTTGCGGCGCTTTACTTAGCTTATCGTATGTTTTGTGGTGT
TTTTTTAGAAATACGGTAAG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMDAQVLDSMDLERERGITIKAQSVTLEYKAKNGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALDMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMNAVR
CSAKTGVGIDEVLEVIVDQIPPPEGDPEAPLQALIIDSWFDSYLGVVSLVRIKNGVLKKGDKFKVMSTGQNHTADRVGIF
TPKQTDKTELKTGEVGFVIAGIKEIHGAPVGDTLTLAKHGAEKPLPGFKKVKPQVYAGVFPISTDEYENFRDALNKLSLN
DASLFFEPESSSALGFGFRIGYLGLLHMEIIQERLEREYDLDLITTAPTVVYEVLLTSGETIYVDNPADLPAINNIEEMR
EPIVEANILVPKEYLGNVITLCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
VRLDILINGDRVDALAMIIHRSNIRHRGLALVEKMKELIPRQMFDIAIQAAVGSQIIARSTVKALRKDVTAKCYGGDVSR
KKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE

Sequences:

>Translated_596_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMDAQVLDSMDLERERGITIKAQSVTLEYKAKNGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALDMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMNAVR
CSAKTGVGIDEVLEVIVDQIPPPEGDPEAPLQALIIDSWFDSYLGVVSLVRIKNGVLKKGDKFKVMSTGQNHTADRVGIF
TPKQTDKTELKTGEVGFVIAGIKEIHGAPVGDTLTLAKHGAEKPLPGFKKVKPQVYAGVFPISTDEYENFRDALNKLSLN
DASLFFEPESSSALGFGFRIGYLGLLHMEIIQERLEREYDLDLITTAPTVVYEVLLTSGETIYVDNPADLPAINNIEEMR
EPIVEANILVPKEYLGNVITLCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
VRLDILINGDRVDALAMIIHRSNIRHRGLALVEKMKELIPRQMFDIAIQAAVGSQIIARSTVKALRKDVTAKCYGGDVSR
KKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE
>Mature_596_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMDAQVLDSMDLERERGITIKAQSVTLEYKAKNGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALDMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMNAVR
CSAKTGVGIDEVLEVIVDQIPPPEGDPEAPLQALIIDSWFDSYLGVVSLVRIKNGVLKKGDKFKVMSTGQNHTADRVGIF
TPKQTDKTELKTGEVGFVIAGIKEIHGAPVGDTLTLAKHGAEKPLPGFKKVKPQVYAGVFPISTDEYENFRDALNKLSLN
DASLFFEPESSSALGFGFRIGYLGLLHMEIIQERLEREYDLDLITTAPTVVYEVLLTSGETIYVDNPADLPAINNIEEMR
EPIVEANILVPKEYLGNVITLCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
VRLDILINGDRVDALAMIIHRSNIRHRGLALVEKMKELIPRQMFDIAIQAAVGSQIIARSTVKALRKDVTAKCYGGDVSR
KKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=48.3388704318937, Blast_Score=612, Evalue=1e-175,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.8646616541353, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=46.2686567164179, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=46.2686567164179, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=46.2686567164179, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.9722222222222, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI18390331, Length=150, Percent_Identity=38, Blast_Score=101, Evalue=3e-21,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=43.6363636363636, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=43.6363636363636, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=43.6363636363636, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI53729339, Length=222, Percent_Identity=31.981981981982, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI53729337, Length=222, Percent_Identity=31.981981981982, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=37.593984962406, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=37.593984962406, Blast_Score=83, Evalue=1e-15,
Organism=Homo sapiens, GI34147630, Length=311, Percent_Identity=26.6881028938907, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=81.2080536912752, Blast_Score=981, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=27.9527559055118, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=35.4838709677419, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789559, Length=225, Percent_Identity=32.4444444444444, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=31.4102564102564, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789737, Length=335, Percent_Identity=27.1641791044776, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790412, Length=335, Percent_Identity=27.1641791044776, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=40.8196721311475, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17556745, Length=465, Percent_Identity=24.3010752688172, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=37.6712328767123, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=35.6687898089172, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=36.5671641791045, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=36.5671641791045, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.8620689655172, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=323, Percent_Identity=28.1733746130031, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI32566303, Length=247, Percent_Identity=27.1255060728745, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=222, Percent_Identity=28.3783783783784, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=610, Percent_Identity=45.7377049180328, Blast_Score=549, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=43.0555555555556, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=43.0555555555556, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=36.0655737704918, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=38.2608695652174, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=38.1944444444444, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=336, Percent_Identity=26.4880952380952, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=273, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319594, Length=273, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=44.6843853820598, Blast_Score=540, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24582462, Length=186, Percent_Identity=34.4086021505376, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=43.8848920863309, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585709, Length=170, Percent_Identity=34.1176470588235, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585711, Length=170, Percent_Identity=34.1176470588235, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585713, Length=170, Percent_Identity=34.1176470588235, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=34.8148148148148, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=243, Percent_Identity=30.8641975308642, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281363316, Length=324, Percent_Identity=27.4691358024691, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17864358, Length=324, Percent_Identity=27.4691358024691, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 65972; Mature: 65972

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMDAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHCCCEEEEEEEE
LEYKAKNGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
DMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMNAVRCSAKTGVGIDEVLEVIVDQI
CCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEECCCCCCCHHHHHHHHHHHC
PPPEGDPEAPLQALIIDSWFDSYLGVVSLVRIKNGVLKKGDKFKVMSTGQNHTADRVGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEE
TPKQTDKTELKTGEVGFVIAGIKEIHGAPVGDTLTLAKHGAEKPLPGFKKVKPQVYAGVF
CCCCCCCCCCCCCCCEEEEECHHHHCCCCCCCCHHHHHCCCCCCCCCHHHCCCCCEEEEE
PISTDEYENFRDALNKLSLNDASLFFEPESSSALGFGFRIGYLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVLLTSGETIYVDNPADLPAINNIEEMREPIVEANILVPKEYLGNVIT
CEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEECCHHHHHHHHH
LCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
HHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCE
VRLDILINGDRVDALAMIIHRSNIRHRGLALVEKMKELIPRQMFDIAIQAAVGSQIIARS
EEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVKALRKDVTAKCYGGDVSRKKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEECC
>Mature Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMDAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHCCCEEEEEEEE
LEYKAKNGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
DMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMNAVRCSAKTGVGIDEVLEVIVDQI
CCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEECCCCCCCHHHHHHHHHHHC
PPPEGDPEAPLQALIIDSWFDSYLGVVSLVRIKNGVLKKGDKFKVMSTGQNHTADRVGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEE
TPKQTDKTELKTGEVGFVIAGIKEIHGAPVGDTLTLAKHGAEKPLPGFKKVKPQVYAGVF
CCCCCCCCCCCCCCCEEEEECHHHHCCCCCCCCHHHHHCCCCCCCCCHHHCCCCCEEEEE
PISTDEYENFRDALNKLSLNDASLFFEPESSSALGFGFRIGYLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVLLTSGETIYVDNPADLPAINNIEEMREPIVEANILVPKEYLGNVIT
CEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEECCHHHHHHHHH
LCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
HHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCE
VRLDILINGDRVDALAMIIHRSNIRHRGLALVEKMKELIPRQMFDIAIQAAVGSQIIARS
EEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVKALRKDVTAKCYGGDVSRKKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA