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The map label for this gene is hsdM [H]

Identifier: 117921401

GI number: 117921401

Start: 3555768

End: 3557360

Strand: Reverse

Name: hsdM [H]

Synonym: Shewana3_2961

Alternate gene names: 117921401

Gene position: 3557360-3555768 (Counterclockwise)

Preceding gene: 117921402

Following gene: 117921400

Centisome position: 71.54

GC content: 49.84

Gene sequence:

>1593_bases
ATGACCCAAAACGATATCGTTCAAAAACTCTGGAACCTCTGTGACATCCTCCGTGATGATGGCATTAACTATTCTGATTA
TGTCACCGAGCTAGTCTTGCTGCTGTTTATCAAAATGGTGCACGAAAATACCGAAGCCGAACTCTTGGATAAACATACCT
TGCCAGAGGGGTATCGCTGGACCGATTTACACACTAAATCCGGCATCAACCTGCTTAACGACTACAAAGCACTGTTACTG
GCATTATCCACAGGTAAACGGGTCATGCTCGACCCTAATGACCCAGAAAAAACCATTGAAGTCCAAGTACACAACGACCC
GTTAATCAGCGCTATCTATGCCGATGCGCAAACCCGTTTGCGTGAGCCACGCCATTTAGAGCAGATCACTAAAACCCTCG
ACCAAATTGATTGGTTTAGCGCAAAGCGCGATGGGTTAGGTGATTTATACGAAGGCTTATTAGAGAAAAACGCCAGTGAG
ACAAAATCGGGTGCAGGACAATACTTTACCCCGCGTGTATTAATCAACAGTATGGTGCGTTGCATCAAACCCCAAGCTGG
CGAATACATTCAGGACCCAGCAGCGGGCACCGCAGGCTTTTTGATTGCCGCCCATGAGTATATTAAAGCGCAACCTGAAT
ATGATGATCTAAGCCTGAAACAAATCGATTTTCAGCGTTATCACGCCTATGTCGGGGTAGAGCTCGTCCCCAATACCCGC
CGCTTAGCCTTAATGAACTGTTTGCTTCACGGCATGGAAGGCGATGACGATGGCGTAGTGCATCTAGGCAACGCCTTAGG
CAATGTCGGTCAAAGCCTCAAACCTGCTGATGTCATTTTGGCTAATCCGCCCTTTGGTACCAGCAAAGGCGGCGAGGCCA
GTATTACCCGTGATGATTTAACCTTTGATACCAGCAACAAACAGTTGGCATTCTTACAACACATTTACCGCAACCTAAAA
CCCGGTGGCCGCGCCGCCGTGGTGCTGCCCGACAACGTGTTGTTTGAAGCTGGTAAGGGTACCGATATCCGCCGCGATTT
AATGGATAAGTGCAACCTGCACACTATCCTGCGCCTGCCCACTGGTATCTTTTATGCCCAGGGTGTGAAAACTAACGTGC
TTTTTTTCACCAAAGGATCTGCAAAAGACAAACACCAGCAAGAACAGTGTACCGAAAACGTCTGGGTCTATGACCTTCGT
ACCAATATGCCAAGCTTTGGCAAACGAACTCCGTTTAGCGATAACCACTTAGCACCATTTGAACAGGTTTACGGCGAACA
TGCTGGTGGACTCAGCCCACGTACTGAAGGGGAATATAGCTTCGGTGCTGAGCAGGTGGAAATCGCTGACACCGACGAAA
ATCAGGGCGTGGATAATAGGCTGGTACACTCCCGTTGGCGCTGTTTCAGCCGCGACTGGATCCGTGACCACAAGGGCGAC
TCGCTAGATATCAGTTGGCTGAAAGACAAAGACAGCGTCGATGCCGCCAGCCTACCCGAGCCCGATGTACTGGCTAGGGA
AGCCAAGGCGGAGCTAAGCGCTGCGCTCAACGAGCTGGATGCATTGCTGAAAGCCTTGGGAGGGGCAGTGTGA

Upstream 100 bases:

>100_bases
TTGTGCACCCTAAACTGATGCAATTGATGACGCGCGATAGCAACCAAATTAAGCCGTACACCATGGCCGAATAAGCCACA
GCAACAAAATTGAGAACAAC

Downstream 100 bases:

>100_bases
TGGTTGAGGTCAATCTGCCCAAAGGCTGGGCTGTTACAACCATTGGGGCAGTAGCTAGAGTCTCAAGTGGCGTTGGCTTT
CCAATTAAATATCAGGGTAA

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoKI [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTQNDIVQKLWNLCDILRDDGINYSDYVTELVLLLFIKMVHENTEAELLDKHTLPEGYRWTDLHTKSGINLLNDYKALLL
ALSTGKRVMLDPNDPEKTIEVQVHNDPLISAIYADAQTRLREPRHLEQITKTLDQIDWFSAKRDGLGDLYEGLLEKNASE
TKSGAGQYFTPRVLINSMVRCIKPQAGEYIQDPAAGTAGFLIAAHEYIKAQPEYDDLSLKQIDFQRYHAYVGVELVPNTR
RLALMNCLLHGMEGDDDGVVHLGNALGNVGQSLKPADVILANPPFGTSKGGEASITRDDLTFDTSNKQLAFLQHIYRNLK
PGGRAAVVLPDNVLFEAGKGTDIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFTKGSAKDKHQQEQCTENVWVYDLR
TNMPSFGKRTPFSDNHLAPFEQVYGEHAGGLSPRTEGEYSFGAEQVEIADTDENQGVDNRLVHSRWRCFSRDWIRDHKGD
SLDISWLKDKDSVDAASLPEPDVLAREAKAELSAALNELDALLKALGGAV

Sequences:

>Translated_530_residues
MTQNDIVQKLWNLCDILRDDGINYSDYVTELVLLLFIKMVHENTEAELLDKHTLPEGYRWTDLHTKSGINLLNDYKALLL
ALSTGKRVMLDPNDPEKTIEVQVHNDPLISAIYADAQTRLREPRHLEQITKTLDQIDWFSAKRDGLGDLYEGLLEKNASE
TKSGAGQYFTPRVLINSMVRCIKPQAGEYIQDPAAGTAGFLIAAHEYIKAQPEYDDLSLKQIDFQRYHAYVGVELVPNTR
RLALMNCLLHGMEGDDDGVVHLGNALGNVGQSLKPADVILANPPFGTSKGGEASITRDDLTFDTSNKQLAFLQHIYRNLK
PGGRAAVVLPDNVLFEAGKGTDIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFTKGSAKDKHQQEQCTENVWVYDLR
TNMPSFGKRTPFSDNHLAPFEQVYGEHAGGLSPRTEGEYSFGAEQVEIADTDENQGVDNRLVHSRWRCFSRDWIRDHKGD
SLDISWLKDKDSVDAASLPEPDVLAREAKAELSAALNELDALLKALGGAV
>Mature_529_residues
TQNDIVQKLWNLCDILRDDGINYSDYVTELVLLLFIKMVHENTEAELLDKHTLPEGYRWTDLHTKSGINLLNDYKALLLA
LSTGKRVMLDPNDPEKTIEVQVHNDPLISAIYADAQTRLREPRHLEQITKTLDQIDWFSAKRDGLGDLYEGLLEKNASET
KSGAGQYFTPRVLINSMVRCIKPQAGEYIQDPAAGTAGFLIAAHEYIKAQPEYDDLSLKQIDFQRYHAYVGVELVPNTRR
LALMNCLLHGMEGDDDGVVHLGNALGNVGQSLKPADVILANPPFGTSKGGEASITRDDLTFDTSNKQLAFLQHIYRNLKP
GGRAAVVLPDNVLFEAGKGTDIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFTKGSAKDKHQQEQCTENVWVYDLRT
NMPSFGKRTPFSDNHLAPFEQVYGEHAGGLSPRTEGEYSFGAEQVEIADTDENQGVDNRLVHSRWRCFSRDWIRDHKGDS
LDISWLKDKDSVDAASLPEPDVLAREAKAELSAALNELDALLKALGGAV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=537, Percent_Identity=57.5418994413408, Blast_Score=615, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 59235; Mature: 59104

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNDIVQKLWNLCDILRDDGINYSDYVTELVLLLFIKMVHENTEAELLDKHTLPEGYRW
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEE
TDLHTKSGINLLNDYKALLLALSTGKRVMLDPNDPEKTIEVQVHNDPLISAIYADAQTRL
EECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHH
REPRHLEQITKTLDQIDWFSAKRDGLGDLYEGLLEKNASETKSGAGQYFTPRVLINSMVR
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHH
CIKPQAGEYIQDPAAGTAGFLIAAHEYIKAQPEYDDLSLKQIDFQRYHAYVGVELVPNTR
HHCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEEEECCCCH
RLALMNCLLHGMEGDDDGVVHLGNALGNVGQSLKPADVILANPPFGTSKGGEASITRDDL
HHHHHHHHHHCCCCCCCCEEEECHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEECCCC
TFDTSNKQLAFLQHIYRNLKPGGRAAVVLPDNVLFEAGKGTDIRRDLMDKCNLHTILRLP
EEECCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHCCCCEEECCC
TGIFYAQGVKTNVLFFTKGSAKDKHQQEQCTENVWVYDLRTNMPSFGKRTPFSDNHLAPF
CCCEEECCCCEEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCH
EQVYGEHAGGLSPRTEGEYSFGAEQVEIADTDENQGVDNRLVHSRWRCFSRDWIRDHKGD
HHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
SLDISWLKDKDSVDAASLPEPDVLAREAKAELSAALNELDALLKALGGAV
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQNDIVQKLWNLCDILRDDGINYSDYVTELVLLLFIKMVHENTEAELLDKHTLPEGYRW
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEE
TDLHTKSGINLLNDYKALLLALSTGKRVMLDPNDPEKTIEVQVHNDPLISAIYADAQTRL
EECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHH
REPRHLEQITKTLDQIDWFSAKRDGLGDLYEGLLEKNASETKSGAGQYFTPRVLINSMVR
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHH
CIKPQAGEYIQDPAAGTAGFLIAAHEYIKAQPEYDDLSLKQIDFQRYHAYVGVELVPNTR
HHCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEEEEECCCCH
RLALMNCLLHGMEGDDDGVVHLGNALGNVGQSLKPADVILANPPFGTSKGGEASITRDDL
HHHHHHHHHHCCCCCCCCEEEECHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEECCCC
TFDTSNKQLAFLQHIYRNLKPGGRAAVVLPDNVLFEAGKGTDIRRDLMDKCNLHTILRLP
EEECCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHCCCCEEECCC
TGIFYAQGVKTNVLFFTKGSAKDKHQQEQCTENVWVYDLRTNMPSFGKRTPFSDNHLAPF
CCCEEECCCCEEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCH
EQVYGEHAGGLSPRTEGEYSFGAEQVEIADTDENQGVDNRLVHSRWRCFSRDWIRDHKGD
HHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
SLDISWLKDKDSVDAASLPEPDVLAREAKAELSAALNELDALLKALGGAV
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3323532; 7610040; 9278503 [H]