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The map label for this gene is hsdS [H]

Identifier: 117921400

GI number: 117921400

Start: 3554005

End: 3555768

Strand: Reverse

Name: hsdS [H]

Synonym: Shewana3_2960

Alternate gene names: 117921400

Gene position: 3555768-3554005 (Counterclockwise)

Preceding gene: 117921401

Following gene: 117921399

Centisome position: 71.51

GC content: 44.33

Gene sequence:

>1764_bases
ATGGTTGAGGTCAATCTGCCCAAAGGCTGGGCTGTTACAACCATTGGGGCAGTAGCTAGAGTCTCAAGTGGCGTTGGCTT
TCCAATTAAATATCAGGGTAAATCAGAAGGTTTATACCCGGTTTATAAAGTTGGAGATGTCTCGAAAGCTGTTACCAGCA
AACATGGAAACTTGGCTGTTGCTGGTCATTACGTGGATAAAGAAGAAGCTGCTGAATTAAAAGGGGAGATCTTCCCTGTT
GGTGCTACATTGTTTGCAAAAATCGGCGAGGCAGTAAAACTAAATAGAAGGGCATTTGTAAGAAAACCAGGTTTGGCTGA
TAACAATGTTATGGCTGTCATCCCAGACAAATCTGATTGCAATAGATTTTTATATCAGTTTTTGCGAGCGATTGATTTAA
CTGAAACGTCTAGGTCCACGACAGTTCCTTCAATTCGAAAAGGTGATATAGAAGACATTGAGTTATACCTTCCCCCCCTA
GCCGAACAAATAGTCATCGCCGACAAGCTGGATACCCTGCTGGCGCAGGTGGAAACCACCAAAGCCCGCCTCGAACGCAT
CCCCGAGATCCTAAAAAGCTTTCGCCAATCCGTCCTGTCTGCGGCAGTCAGCGGCAAGTTGACGCAAGAGTGGCGGGAAA
GTCATGGCAATGGTACAGGCGAAGAGGTCGTTAAAGCTGATGCAATAAACAAGTCAGTTTTATTGAATGAAAATCCTGCA
CTTAAAAAAAAGAAATCAACAATAGAGAGTCAAATCGATACAGAGTATATATTTGACTTGCCTGAGTCTTGGGGGTTTAC
CACTTGGGGAAAGATTTCTGAATGGATTACATATGGTTTTACAAAACCCATGCCTAAATCAGACTCAGGAGTAAAACTAT
TAACTGCAAAGGACGTGCAGTATTTTGACGTAAATATAAATGACGCAGGACTTACAACAAGTTCTGCGTTTCAAAGTCTT
TCAGATAAAGACAGACCAATAAAAGGCGACTTGTTAATAACAAAAGATGGCAGTATTGGTCGGGCCGCTTTGGTTAGGAC
TGATGAGCCGTTTTGTATTAACCAAAGTGTTGCAGTGTGTTGGTTACGTTCAACTTCTATGAACAAAGATTATCTTGAGT
TTTTAGCCAATAGTGAATTCACACAAAGATTTGTTAAAGACAAAGCTCAAGGGATGGCAATCCAGCACTTATCTATTATT
GATTATGCTAAATGTCCACTTCCAGTCCCATCATTAGAAGAACAAACCGAAATCGTCCGCCGTGTTGAAGAACTGTTCGC
CTTCGCTGACAGCATTGAGCAAAAAGCCACGGCTGCGCTGGCGCGGGTGAATAACCTGACCCAGTCGATTCTGGCCAAGG
CCTTCAGGGGTGAACTGACCGCCGATTGGCGCGCCGCCAACCCTGAGCTTATCAGTGGTGACAACAGCGCCGCAGCCTTA
TTAGAGAAAATTAAGGTGGAACGTGAGGTCATGAAAAAGCAGCCAAAACCGAAACGCAGCAACATAAAAAAAAAGACTGG
TAGCCATATGAGTAAGCAGATCATCAAAGTCGTTGATGCATTAAAAGAGGCCGGTGAACCACTAAGTGGCCAACAGTTAT
TGGCCGCTGCGGGATATCCCAGTGATAGTGGTACCGAACAACTGGAACAGTTCTTTCTGGATATTCGTAAGGCGCTCATC
TCCGAAAAATCCATTGTGAAGTTGCAGCGCGGTGACGATGGACAGGACTGGTTTGCCCTAGCACAGAAGTCTGCCAAAGG
ATAA

Upstream 100 bases:

>100_bases
TACCCGAGCCCGATGTACTGGCTAGGGAAGCCAAGGCGGAGCTAAGCGCTGCGCTCAACGAGCTGGATGCATTGCTGAAA
GCCTTGGGAGGGGCAGTGTG

Downstream 100 bases:

>100_bases
TAGCGAGCCAAGGACAGCCGATGAAAGTCGATAAATTACACATTCGTTCTCGATTTAAAAATTTGGAAAACGTAAAGGTG
GACTTTGATGAAGACCACCT

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.EcoBI; Type I restriction enzyme EcoBI specificity protein; S protein [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MVEVNLPKGWAVTTIGAVARVSSGVGFPIKYQGKSEGLYPVYKVGDVSKAVTSKHGNLAVAGHYVDKEEAAELKGEIFPV
GATLFAKIGEAVKLNRRAFVRKPGLADNNVMAVIPDKSDCNRFLYQFLRAIDLTETSRSTTVPSIRKGDIEDIELYLPPL
AEQIVIADKLDTLLAQVETTKARLERIPEILKSFRQSVLSAAVSGKLTQEWRESHGNGTGEEVVKADAINKSVLLNENPA
LKKKKSTIESQIDTEYIFDLPESWGFTTWGKISEWITYGFTKPMPKSDSGVKLLTAKDVQYFDVNINDAGLTTSSAFQSL
SDKDRPIKGDLLITKDGSIGRAALVRTDEPFCINQSVAVCWLRSTSMNKDYLEFLANSEFTQRFVKDKAQGMAIQHLSII
DYAKCPLPVPSLEEQTEIVRRVEELFAFADSIEQKATAALARVNNLTQSILAKAFRGELTADWRAANPELISGDNSAAAL
LEKIKVEREVMKKQPKPKRSNIKKKTGSHMSKQIIKVVDALKEAGEPLSGQQLLAAAGYPSDSGTEQLEQFFLDIRKALI
SEKSIVKLQRGDDGQDWFALAQKSAKG

Sequences:

>Translated_587_residues
MVEVNLPKGWAVTTIGAVARVSSGVGFPIKYQGKSEGLYPVYKVGDVSKAVTSKHGNLAVAGHYVDKEEAAELKGEIFPV
GATLFAKIGEAVKLNRRAFVRKPGLADNNVMAVIPDKSDCNRFLYQFLRAIDLTETSRSTTVPSIRKGDIEDIELYLPPL
AEQIVIADKLDTLLAQVETTKARLERIPEILKSFRQSVLSAAVSGKLTQEWRESHGNGTGEEVVKADAINKSVLLNENPA
LKKKKSTIESQIDTEYIFDLPESWGFTTWGKISEWITYGFTKPMPKSDSGVKLLTAKDVQYFDVNINDAGLTTSSAFQSL
SDKDRPIKGDLLITKDGSIGRAALVRTDEPFCINQSVAVCWLRSTSMNKDYLEFLANSEFTQRFVKDKAQGMAIQHLSII
DYAKCPLPVPSLEEQTEIVRRVEELFAFADSIEQKATAALARVNNLTQSILAKAFRGELTADWRAANPELISGDNSAAAL
LEKIKVEREVMKKQPKPKRSNIKKKTGSHMSKQIIKVVDALKEAGEPLSGQQLLAAAGYPSDSGTEQLEQFFLDIRKALI
SEKSIVKLQRGDDGQDWFALAQKSAKG
>Mature_587_residues
MVEVNLPKGWAVTTIGAVARVSSGVGFPIKYQGKSEGLYPVYKVGDVSKAVTSKHGNLAVAGHYVDKEEAAELKGEIFPV
GATLFAKIGEAVKLNRRAFVRKPGLADNNVMAVIPDKSDCNRFLYQFLRAIDLTETSRSTTVPSIRKGDIEDIELYLPPL
AEQIVIADKLDTLLAQVETTKARLERIPEILKSFRQSVLSAAVSGKLTQEWRESHGNGTGEEVVKADAINKSVLLNENPA
LKKKKSTIESQIDTEYIFDLPESWGFTTWGKISEWITYGFTKPMPKSDSGVKLLTAKDVQYFDVNINDAGLTTSSAFQSL
SDKDRPIKGDLLITKDGSIGRAALVRTDEPFCINQSVAVCWLRSTSMNKDYLEFLANSEFTQRFVKDKAQGMAIQHLSII
DYAKCPLPVPSLEEQTEIVRRVEELFAFADSIEQKATAALARVNNLTQSILAKAFRGELTADWRAANPELISGDNSAAAL
LEKIKVEREVMKKQPKPKRSNIKKKTGSHMSKQIIKVVDALKEAGEPLSGQQLLAAAGYPSDSGTEQLEQFFLDIRKALI
SEKSIVKLQRGDDGQDWFALAQKSAKG

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=384, Percent_Identity=36.71875, Blast_Score=178, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 64647; Mature: 64647

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVNLPKGWAVTTIGAVARVSSGVGFPIKYQGKSEGLYPVYKVGDVSKAVTSKHGNLAV
CEEECCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCHHHHHHCCCCCEEE
AGHYVDKEEAAELKGEIFPVGATLFAKIGEAVKLNRRAFVRKPGLADNNVMAVIPDKSDC
EECCCCHHHHHHHCCCEEECHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCCHHH
NRFLYQFLRAIDLTETSRSTTVPSIRKGDIEDIELYLPPLAEQIVIADKLDTLLAQVETT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
KARLERIPEILKSFRQSVLSAAVSGKLTQEWRESHGNGTGEEVVKADAINKSVLLNENPA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCC
LKKKKSTIESQIDTEYIFDLPESWGFTTWGKISEWITYGFTKPMPKSDSGVKLLTAKDVQ
HHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCE
YFDVNINDAGLTTSSAFQSLSDKDRPIKGDLLITKDGSIGRAALVRTDEPFCINQSVAVC
EEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCCEECCCEEEE
WLRSTSMNKDYLEFLANSEFTQRFVKDKAQGMAIQHLSIIDYAKCPLPVPSLEEQTEIVR
EEECCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHH
RVEELFAFADSIEQKATAALARVNNLTQSILAKAFRGELTADWRAANPELISGDNSAAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHH
LEKIKVEREVMKKQPKPKRSNIKKKTGSHMSKQIIKVVDALKEAGEPLSGQQLLAAAGYP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCC
SDSGTEQLEQFFLDIRKALISEKSIVKLQRGDDGQDWFALAQKSAKG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVEVNLPKGWAVTTIGAVARVSSGVGFPIKYQGKSEGLYPVYKVGDVSKAVTSKHGNLAV
CEEECCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCHHHHHHCCCCCEEE
AGHYVDKEEAAELKGEIFPVGATLFAKIGEAVKLNRRAFVRKPGLADNNVMAVIPDKSDC
EECCCCHHHHHHHCCCEEECHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCCHHH
NRFLYQFLRAIDLTETSRSTTVPSIRKGDIEDIELYLPPLAEQIVIADKLDTLLAQVETT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
KARLERIPEILKSFRQSVLSAAVSGKLTQEWRESHGNGTGEEVVKADAINKSVLLNENPA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCC
LKKKKSTIESQIDTEYIFDLPESWGFTTWGKISEWITYGFTKPMPKSDSGVKLLTAKDVQ
HHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCE
YFDVNINDAGLTTSSAFQSLSDKDRPIKGDLLITKDGSIGRAALVRTDEPFCINQSVAVC
EEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCCEECCCEEEE
WLRSTSMNKDYLEFLANSEFTQRFVKDKAQGMAIQHLSIIDYAKCPLPVPSLEEQTEIVR
EEECCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHH
RVEELFAFADSIEQKATAALARVNNLTQSILAKAFRGELTADWRAANPELISGDNSAAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHH
LEKIKVEREVMKKQPKPKRSNIKKKTGSHMSKQIIKVVDALKEAGEPLSGQQLLAAAGYP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCC
SDSGTEQLEQFFLDIRKALISEKSIVKLQRGDDGQDWFALAQKSAKG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321 [H]