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The map label for this gene is aceA [H]

Identifier: 117921382

GI number: 117921382

Start: 3531910

End: 3533232

Strand: Reverse

Name: aceA [H]

Synonym: Shewana3_2942

Alternate gene names: 117921382

Gene position: 3533232-3531910 (Counterclockwise)

Preceding gene: 117921383

Following gene: 117921381

Centisome position: 71.06

GC content: 50.42

Gene sequence:

>1323_bases
ATGACTAAGGCAACACAGACTTCACGTCAGGCACAGATTGACGCGATCAAAAAAGATTGGGCTGAAAATCCACGTTGGAA
AAACGTGCGTCGCCCATACACTGCAGAGGAAGTTGTGGCACTTCGTGGTTCTATCGTACCCGAAAACACTATTGCCAAAC
GCGGTGCCGCTAAACTGTGGGATTTAGTCAACGGTGGCGCAAAAAAAGGTTATGTGAACTCGCTAGGTGCACTGACTGGC
GGTCAAGCGGTACAACAGGCGAAAGCCGGTATCGAAGCAATTTACTTGTCTGGTTGGCAAGTGGCGGCCGACGCTAACTT
AGCGGGCACTATGTACCCAGACCAATCTTTATACCCAGCAAACTCAGTACCTGCAGTGGTTGCACGTATTAACAACTCTT
TCCGCCGTGCTGACCAAATTCAGTGGAGCAATGGTGTTAATCCGGAAGAAGAAAACTTTGTCGATTACTTCCTGCCGATT
ATTGCCGATGCGGAAGCGGGTTTTGGTGGCGTACTGAATGCGTTCGAGTTAATGAAGTCGATGATCGACGCAGGCGCCGC
CGGTGTGCACTTTGAAGACCAATTGGCCTCTGTGAAAAAGTGCGGCCATATGGGCGGTAAAGTATTAGTACCGACCCAAG
AAGCGGTACAAAAGTTAGTTGCGGCGCGCCTTGCAGCCGACGTGAGCGGTGTTGAAACTTTAGTTATCGCTCGTACCGAT
GCGAATGCGGCGGATTTATTGACCTCTGATTGCGACCCATACGATCGTGACTTCGTGACTGGCGAGCGCACCAGTGAAGG
TTTCTACCGCGTGAAAGCGGGTCTGGACCAAGCGATTTCTCGCGGTCTCGCCTACGCACCTTATGCGGATTTAATTTGGT
GTGAAACCGCTAAGCCTGACTTAGAAGAAGCACGCCGCTTTGCTGAGGCAATCCATGCTCAGTACCCAGACCAATTGCTG
GCCTACAACTGTTCACCTTCGTTCAACTGGAAGAAAAACTTGGACGACGCGACGATTGCGCGCTTCCAACAAGAGCTGTC
GGACATGGGCTACAAGTACCAGTTCATCACTTTAGCGGGTATCCATAACATGTGGTACAACATGTTTGACCTGGCTTACG
ACTACGCTCGTGGTGAAGGTATGAAGCATTATGTTGAGAAAGTTCAAGAAGTTGAGTTTGCAGCAGCGAAGAAAGGCTAC
ACCTTCGTCGCGCATCAACAGGAAGTGGGTACTGGTTACTTCGACCAAGTCACCACAGTGATCCAAGGCGGTCAGTCTTC
TGTGACTGCACTGACTGGTTCGACCGAAGAAGAGCAGTTTTAA

Upstream 100 bases:

>100_bases
AATGCGTTATGGCGGTCAAACCGCCAATTAAACCAATGGGCAGGGTGAGGCTGTGGGGCAGCTTCACTTGCTACAACATC
ACAGAGAAAGGAGTGACACT

Downstream 100 bases:

>100_bases
GCGAAGTCTGTTGTATAGCGAATAGGTTCTACCCGTAAGATGTTGTACCCGTAAGCCCTGTGTTTAGCTGCATTGCAGCT
GTTGTCCCCCGTGCAGTTCG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTKATQTSRQAQIDAIKKDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKLWDLVNGGAKKGYVNSLGALTG
GQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANSVPAVVARINNSFRRADQIQWSNGVNPEEENFVDYFLPI
IADAEAGFGGVLNAFELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTD
ANAADLLTSDCDPYDRDFVTGERTSEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKPDLEEARRFAEAIHAQYPDQLL
AYNCSPSFNWKKNLDDATIARFQQELSDMGYKYQFITLAGIHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKGY
TFVAHQQEVGTGYFDQVTTVIQGGQSSVTALTGSTEEEQF

Sequences:

>Translated_440_residues
MTKATQTSRQAQIDAIKKDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKLWDLVNGGAKKGYVNSLGALTG
GQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANSVPAVVARINNSFRRADQIQWSNGVNPEEENFVDYFLPI
IADAEAGFGGVLNAFELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTD
ANAADLLTSDCDPYDRDFVTGERTSEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKPDLEEARRFAEAIHAQYPDQLL
AYNCSPSFNWKKNLDDATIARFQQELSDMGYKYQFITLAGIHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKGY
TFVAHQQEVGTGYFDQVTTVIQGGQSSVTALTGSTEEEQF
>Mature_439_residues
TKATQTSRQAQIDAIKKDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKLWDLVNGGAKKGYVNSLGALTGG
QAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANSVPAVVARINNSFRRADQIQWSNGVNPEEENFVDYFLPII
ADAEAGFGGVLNAFELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTDA
NAADLLTSDCDPYDRDFVTGERTSEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKPDLEEARRFAEAIHAQYPDQLLA
YNCSPSFNWKKNLDDATIARFQQELSDMGYKYQFITLAGIHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKGYT
FVAHQQEVGTGYFDQVTTVIQGGQSSVTALTGSTEEEQF

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=433, Percent_Identity=79.2147806004619, Blast_Score=742, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=266, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71982926, Length=423, Percent_Identity=59.1016548463357, Blast_Score=494, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17561814, Length=423, Percent_Identity=59.1016548463357, Blast_Score=493, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6320908, Length=312, Percent_Identity=35.5769230769231, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6325263, Length=256, Percent_Identity=38.671875, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48290; Mature: 48158

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00074 GLFV_DEHYDROGENASE ; PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKATQTSRQAQIDAIKKDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKLW
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
DLVNGGAKKGYVNSLGALTGGQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPA
HHHCCCCCCCHHHHHHCCCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCC
NSVPAVVARINNSFRRADQIQWSNGVNPEEENFVDYFLPIIADAEAGFGGVLNAFELMKS
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTD
HHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECC
ANAADLLTSDCDPYDRDFVTGERTSEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKPD
CCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCC
LEEARRFAEAIHAQYPDQLLAYNCSPSFNWKKNLDDATIARFQQELSDMGYKYQFITLAG
HHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHH
IHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKGYTFVAHQQEVGTGYFDQVTTV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHH
IQGGQSSVTALTGSTEEEQF
HHCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TKATQTSRQAQIDAIKKDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKLW
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
DLVNGGAKKGYVNSLGALTGGQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPA
HHHCCCCCCCHHHHHHCCCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCC
NSVPAVVARINNSFRRADQIQWSNGVNPEEENFVDYFLPIIADAEAGFGGVLNAFELMKS
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVETLVIARTD
HHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECC
ANAADLLTSDCDPYDRDFVTGERTSEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKPD
CCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCC
LEEARRFAEAIHAQYPDQLLAYNCSPSFNWKKNLDDATIARFQQELSDMGYKYQFITLAG
HHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHH
IHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKGYTFVAHQQEVGTGYFDQVTTV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHH
IQGGQSSVTALTGSTEEEQF
HHCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]