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The map label for this gene is rlmM [H]

Identifier: 117921332

GI number: 117921332

Start: 3461304

End: 3462389

Strand: Reverse

Name: rlmM [H]

Synonym: Shewana3_2891

Alternate gene names: 117921332

Gene position: 3462389-3461304 (Counterclockwise)

Preceding gene: 117921333

Following gene: 117921330

Centisome position: 69.63

GC content: 50.0

Gene sequence:

>1086_bases
ATGAAAAACCTATTTTTATTTTGCCGTGCAGGCTATGAGAAAGAATGCGCAGCAGAAATCCAACAGCGAGCCGCCGAGCT
CAATGTGGGTGGCTTTGTTAAGGCCAACAACAACGATGCCTACGTGGTATATCAATGTTTTGAAGAAGACGGCGGCGATA
CCTTAGTGAAGCAACTGCCGTTAGATTCACTGATTTTTGCCCGTCAGATGTTTGCCGCAAGCGAGCTGCTGGCCGATTTA
CCCGAGTCTGATCGTGTCAGCCCGATTGTGGCGGCGCTCAGTGAGGTGAGTAAAGCGGGTGAGCTGAGGGTTGAAACCCC
TGATACCAACGAAGCGAAAGAGCTGTCGGCATTTTGCCGTAAGTTTACCGTGCCTTTGCGCCAGCATTTGAAAAAATCCG
GCAGTTTGCTGGCACAGGAAAATCCTAAGCGTCCGATTATCCACGTGTGTTTTATCGGCCCAGGTCGCGCCTACGTAGGT
TATTCCTTCAGTAACAACAGCTCACCCTATTTTATGGGGATCCCGCGCCTGAAAATGGCTGCCGATGCGCCGAGCCGCTC
AAGCTTGAAACTCGATGAAGCCTTCGCGCAGTTTGTGCCTAAAGAAGAGCAAGAAGTGCGGGTTCGCAGCGGCATGAACG
CAGTTGACTTAGGCGCTTGCCCTGGCGGCTGGACCTATCAATTAGTGCGTCGTGGCATGATGGTCTCCGCAGTCGATAAT
GGCCCGATGAACGAAAAGCTGATGGAAACCGGACAAGTGAAACATTTCCGCGAGGATGGTTTCCGCTTTGAGCCACAGCG
TAAAAACATTTATTGGTTAGTGTGTGACATGGTTGAAAAACCCGCGCGCGTGGCTGAGCTGATTGAAGCTTGGGCCATCA
ATGGCTGGTTTAAAGAAGCGATTTTCAACCTCAAACTGCCGATGAAGAGCCGTTATAAAGAAGTGATGGCAATTTTGAAT
ACCATGCAGGAAATCCTCAAGGAAAACGGCATCAACGAATTCCAGTTACAGTGTAAGCACTTATACCATGACCGTGATGA
AGTGACTGTTCACCTGTGGATTAGACCGAGCCAGGCTTGGAACTAA

Upstream 100 bases:

>100_bases
AGCCCGATAGCAAATCGGGCTTTTGTGTATTGAAATTGCTATACTGGCGCCCAAATCGATAGTCGGCCATGTTTATGGCT
TGGATTTCTTAAGGATCTCC

Downstream 100 bases:

>100_bases
TCGGCGGTTTTTGGCCGTTCTGTTAGTGAGTGAAGCCCCAAGTCATGTCTGGGGCTTTTTATTTGGCTGTTCTGCTAGAT
TGCGGCCATAAAAAAACGCA

Product: putative RNA 2'-O-ribose methyltransferase

Products: NA

Alternate protein names: 23S rRNA 2'-O-ribose methyltransferase rlmM [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKNLFLFCRAGYEKECAAEIQQRAAELNVGGFVKANNNDAYVVYQCFEEDGGDTLVKQLPLDSLIFARQMFAASELLADL
PESDRVSPIVAALSEVSKAGELRVETPDTNEAKELSAFCRKFTVPLRQHLKKSGSLLAQENPKRPIIHVCFIGPGRAYVG
YSFSNNSSPYFMGIPRLKMAADAPSRSSLKLDEAFAQFVPKEEQEVRVRSGMNAVDLGACPGGWTYQLVRRGMMVSAVDN
GPMNEKLMETGQVKHFREDGFRFEPQRKNIYWLVCDMVEKPARVAELIEAWAINGWFKEAIFNLKLPMKSRYKEVMAILN
TMQEILKENGINEFQLQCKHLYHDRDEVTVHLWIRPSQAWN

Sequences:

>Translated_361_residues
MKNLFLFCRAGYEKECAAEIQQRAAELNVGGFVKANNNDAYVVYQCFEEDGGDTLVKQLPLDSLIFARQMFAASELLADL
PESDRVSPIVAALSEVSKAGELRVETPDTNEAKELSAFCRKFTVPLRQHLKKSGSLLAQENPKRPIIHVCFIGPGRAYVG
YSFSNNSSPYFMGIPRLKMAADAPSRSSLKLDEAFAQFVPKEEQEVRVRSGMNAVDLGACPGGWTYQLVRRGMMVSAVDN
GPMNEKLMETGQVKHFREDGFRFEPQRKNIYWLVCDMVEKPARVAELIEAWAINGWFKEAIFNLKLPMKSRYKEVMAILN
TMQEILKENGINEFQLQCKHLYHDRDEVTVHLWIRPSQAWN
>Mature_361_residues
MKNLFLFCRAGYEKECAAEIQQRAAELNVGGFVKANNNDAYVVYQCFEEDGGDTLVKQLPLDSLIFARQMFAASELLADL
PESDRVSPIVAALSEVSKAGELRVETPDTNEAKELSAFCRKFTVPLRQHLKKSGSLLAQENPKRPIIHVCFIGPGRAYVG
YSFSNNSSPYFMGIPRLKMAADAPSRSSLKLDEAFAQFVPKEEQEVRVRSGMNAVDLGACPGGWTYQLVRRGMMVSAVDN
GPMNEKLMETGQVKHFREDGFRFEPQRKNIYWLVCDMVEKPARVAELIEAWAINGWFKEAIFNLKLPMKSRYKEVMAILN
TMQEILKENGINEFQLQCKHLYHDRDEVTVHLWIRPSQAWN

Specific function: Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA [H]

COG id: COG2933

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789171, Length=353, Percent_Identity=59.7733711048159, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011224
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: NA

Molecular weight: Translated: 40987; Mature: 40987

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLFLFCRAGYEKECAAEIQQRAAELNVGGFVKANNNDAYVVYQCFEEDGGDTLVKQLP
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCCHHHHHHCC
LDSLIFARQMFAASELLADLPESDRVSPIVAALSEVSKAGELRVETPDTNEAKELSAFCR
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
KFTVPLRQHLKKSGSLLAQENPKRPIIHVCFIGPGRAYVGYSFSNNSSPYFMGIPRLKMA
HHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCEEEC
ADAPSRSSLKLDEAFAQFVPKEEQEVRVRSGMNAVDLGACPGGWTYQLVRRGMMVSAVDN
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCEEEECCC
GPMNEKLMETGQVKHFREDGFRFEPQRKNIYWLVCDMVEKPARVAELIEAWAINGWFKEA
CCCCHHHHHHCCHHHHHHCCCEECCCCCCEEEEEECHHHCHHHHHHHHHHHHCCHHHHHH
IFNLKLPMKSRYKEVMAILNTMQEILKENGINEFQLQCKHLYHDRDEVTVHLWIRPSQAW
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCC
N
C
>Mature Secondary Structure
MKNLFLFCRAGYEKECAAEIQQRAAELNVGGFVKANNNDAYVVYQCFEEDGGDTLVKQLP
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCCHHHHHHCC
LDSLIFARQMFAASELLADLPESDRVSPIVAALSEVSKAGELRVETPDTNEAKELSAFCR
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
KFTVPLRQHLKKSGSLLAQENPKRPIIHVCFIGPGRAYVGYSFSNNSSPYFMGIPRLKMA
HHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCEEEC
ADAPSRSSLKLDEAFAQFVPKEEQEVRVRSGMNAVDLGACPGGWTYQLVRRGMMVSAVDN
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCEEEECCC
GPMNEKLMETGQVKHFREDGFRFEPQRKNIYWLVCDMVEKPARVAELIEAWAINGWFKEA
CCCCHHHHHHCCHHHHHHCCCEECCCCCCEEEEEECHHHCHHHHHHHHHHHHCCHHHHHH
IFNLKLPMKSRYKEVMAILNTMQEILKENGINEFQLQCKHLYHDRDEVTVHLWIRPSQAW
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA