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The map label for this gene is pksE [H]

Identifier: 117921284

GI number: 117921284

Start: 3402696

End: 3404342

Strand: Direct

Name: pksE [H]

Synonym: Shewana3_2843

Alternate gene names: 117921284

Gene position: 3402696-3404342 (Clockwise)

Preceding gene: 117921283

Following gene: 117921285

Centisome position: 68.43

GC content: 53.43

Gene sequence:

>1647_bases
ATGACGAATACCACACTCGATAATAAAAAGCTCAGTCCTTGGCCATGGCAGGTTGATGAAGCCGCCATCAGTTTCGATAT
CGATTCCCTTGGCAAAAAACTTAAAGATCTCAATCAAGCCTGTTACTTAGTCAATCATGCCGAAAAAGGTTTAGGCATAG
CCCAAAGCGCCGAGGTGGTGGGTCTTGCAGAACCCAGCAATAGCTTACACAAAAGCAATGGTTTGCATCCTGTGAGTGCC
TTCGCCCCCGCCCTTGGCACCCAAAGCTTGGGTGACAGTAATTTTCGCCGCGTGCATGGGGTGAAATACGCCTACTACGC
CGGCGCCATGGCCAACGGCATCGCCTCGGAAGAGTTAGTTATCGCCTTAGGTCAGGCGGGCATTCTGTGCTCCTTTGGCG
CGGCAGGGTTAATTCCATCGCGCGTTGAAGCCGCGATTAAACGCATTCAAACGGCATTGCCCAATGGCCCCTACGCCTTT
AACCTGATCCACAGCCCGAGCGAGCCAGCGCTTGAGCGTGGCAGTGTCGAACTCTTCCTTAAGCATCAAGTGCGTACAGT
TGAGGCTTCGGCCTTCTTGGGCTTAACGCCGCAAATCGTCTATTACCGCGCCGCAGGTCTGAGCCGCGATGCCAACGGCG
AGATTGTGATTGGCAACAAAGTGATTGCTAAAATCAGCCGTACCGAGGTGGCCACCAAGTTTATGGAGCCCGCCCCGGTT
AAGATGCTGCAACAATTAGTGAACGAAGGGCTTATCAGCGAAGAGCAAATGTTGATGGCGCAATCTGTGCCCATGGCCGA
TGACATTACCGCCGAAGCCGACTCTGGCGGCCACACCGACAATCGCCCTCTGGTCACGCTATTGCCGACCATTTTGGCGC
TCAAAGATACCATTCAAGCCAAGTACCAGTACAAAACGCCGATCCGTGTGGGCGCAGGTGGTGGGATCGGCACGCCCGAT
GCGGCGCTGGCAACTTTCAATATGGGCGCGGCCTTTATTGTCACCGGCTCAATCAATCAAGCCTGTGTTGAGGCGGGAGC
CAGCGAACATACCCGCAAGTTACTCGCCACCACCGAAATGGCCGATGTGACTATGGCGCCCGCCGCCGATATGTTCGAAA
TGGGCGTTAAGTTACAAGTGGTTAAGCGCGGCACCCTATTCCCGATGCGTGCCAATAAACTCTATGAGATTTACACCCGC
TACGACTCGATTGAGGCGATTCCGGCAGAGGAAAGACAAAAGCTAGAAGAGCAAGTGTTTCGCGCCTCACTAGATGTGAT
TTGGGCAGGTACTGTGGCGCACTTTAATGAGCGCGATCCTAAGCAAATTGAACGCGCGCTGGATAACCCAAAACGCAAAA
TGGCACTGATTTTCCGTTGGTATTTGGGTCTTTCGAGCCGCTGGTCGAATACAGGCGAAGTTGGCCGCGAAATGGATTAC
CAGATTTGGGCAGGCCCCGCCCTCGGCGCCTTTAATGCCTGGGCCAAAGGCAGTTATTTAGATGATTACCGCGAGCGCAA
TGCGGTCGATTTGGCGAAACATTTAATGCAAGGCGCTGCCTATCAAGCACGGATTAACCTGTTGTTATCCCAAGGGGTAA
GTATTCCGGTCAGCTTGCAACGCTGGAAACCACTGCAACGCTGCTAA

Upstream 100 bases:

>100_bases
AACAATTGGTTTCAGAACAATAGACTACCGCAGTGCAAGGGAATAGCCCCACTGCGGCAAACAAATTCAGGCCCCAAACA
CCCAAAAGTGAACAGCCCAA

Downstream 100 bases:

>100_bases
TTCGCTTTGCTAAACCTTATTGAGCGGGTTAAGTGAAAACTTAACCCGCTTTTTTTGACTTCAATTTTTGATTCATTTAT
GGGTCAACTTCCGCTCGGCA

Product: 2-nitropropane dioxygenase, NPD

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTNTTLDNKKLSPWPWQVDEAAISFDIDSLGKKLKDLNQACYLVNHAEKGLGIAQSAEVVGLAEPSNSLHKSNGLHPVSA
FAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELVIALGQAGILCSFGAAGLIPSRVEAAIKRIQTALPNGPYAF
NLIHSPSEPALERGSVELFLKHQVRTVEASAFLGLTPQIVYYRAAGLSRDANGEIVIGNKVIAKISRTEVATKFMEPAPV
KMLQQLVNEGLISEEQMLMAQSVPMADDITAEADSGGHTDNRPLVTLLPTILALKDTIQAKYQYKTPIRVGAGGGIGTPD
AALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTR
YDSIEAIPAEERQKLEEQVFRASLDVIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
QIWAGPALGAFNAWAKGSYLDDYRERNAVDLAKHLMQGAAYQARINLLLSQGVSIPVSLQRWKPLQRC

Sequences:

>Translated_548_residues
MTNTTLDNKKLSPWPWQVDEAAISFDIDSLGKKLKDLNQACYLVNHAEKGLGIAQSAEVVGLAEPSNSLHKSNGLHPVSA
FAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELVIALGQAGILCSFGAAGLIPSRVEAAIKRIQTALPNGPYAF
NLIHSPSEPALERGSVELFLKHQVRTVEASAFLGLTPQIVYYRAAGLSRDANGEIVIGNKVIAKISRTEVATKFMEPAPV
KMLQQLVNEGLISEEQMLMAQSVPMADDITAEADSGGHTDNRPLVTLLPTILALKDTIQAKYQYKTPIRVGAGGGIGTPD
AALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTR
YDSIEAIPAEERQKLEEQVFRASLDVIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
QIWAGPALGAFNAWAKGSYLDDYRERNAVDLAKHLMQGAAYQARINLLLSQGVSIPVSLQRWKPLQRC
>Mature_547_residues
TNTTLDNKKLSPWPWQVDEAAISFDIDSLGKKLKDLNQACYLVNHAEKGLGIAQSAEVVGLAEPSNSLHKSNGLHPVSAF
APALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELVIALGQAGILCSFGAAGLIPSRVEAAIKRIQTALPNGPYAFN
LIHSPSEPALERGSVELFLKHQVRTVEASAFLGLTPQIVYYRAAGLSRDANGEIVIGNKVIAKISRTEVATKFMEPAPVK
MLQQLVNEGLISEEQMLMAQSVPMADDITAEADSGGHTDNRPLVTLLPTILALKDTIQAKYQYKTPIRVGAGGGIGTPDA
ALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRY
DSIEAIPAEERQKLEEQVFRASLDVIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDYQ
IWAGPALGAFNAWAKGSYLDDYRERNAVDLAKHLMQGAAYQARINLLLSQGVSIPVSLQRWKPLQRC

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 59719; Mature: 59588

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTTLDNKKLSPWPWQVDEAAISFDIDSLGKKLKDLNQACYLVNHAEKGLGIAQSAEVV
CCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
GLAEPSNSLHKSNGLHPVSAFAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELV
EECCCCCHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEE
IALGQAGILCSFGAAGLIPSRVEAAIKRIQTALPNGPYAFNLIHSPSEPALERGSVELFL
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEEEE
KHQVRTVEASAFLGLTPQIVYYRAAGLSRDANGEIVIGNKVIAKISRTEVATKFMEPAPV
EHHHHEEEHHHHCCCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHH
KMLQQLVNEGLISEEQMLMAQSVPMADDITAEADSGGHTDNRPLVTLLPTILALKDTIQA
HHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KYQYKTPIRVGAGGGIGTPDAALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEM
CEEECCCEEEECCCCCCCCCHHHEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
ADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIEAIPAEERQKLEEQVF
HHEEECCCHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
RASLDVIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
HHHHHEEEECHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
QIWAGPALGAFNAWAKGSYLDDYRERNAVDLAKHLMQGAAYQARINLLLSQGVSIPVSLQ
EEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEHH
RWKPLQRC
HCCCHHCC
>Mature Secondary Structure 
TNTTLDNKKLSPWPWQVDEAAISFDIDSLGKKLKDLNQACYLVNHAEKGLGIAQSAEVV
CCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
GLAEPSNSLHKSNGLHPVSAFAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELV
EECCCCCHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEE
IALGQAGILCSFGAAGLIPSRVEAAIKRIQTALPNGPYAFNLIHSPSEPALERGSVELFL
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEEEE
KHQVRTVEASAFLGLTPQIVYYRAAGLSRDANGEIVIGNKVIAKISRTEVATKFMEPAPV
EHHHHEEEHHHHCCCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHH
KMLQQLVNEGLISEEQMLMAQSVPMADDITAEADSGGHTDNRPLVTLLPTILALKDTIQA
HHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KYQYKTPIRVGAGGGIGTPDAALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEM
CEEECCCEEEECCCCCCCCCHHHEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
ADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIEAIPAEERQKLEEQVF
HHEEECCCHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
RASLDVIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
HHHHHEEEECHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
QIWAGPALGAFNAWAKGSYLDDYRERNAVDLAKHLMQGAAYQARINLLLSQGVSIPVSLQ
EEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEHH
RWKPLQRC
HCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]