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The map label for this gene is rnhB [H]

Identifier: 117921242

GI number: 117921242

Start: 3347752

End: 3348381

Strand: Reverse

Name: rnhB [H]

Synonym: Shewana3_2801

Alternate gene names: 117921242

Gene position: 3348381-3347752 (Counterclockwise)

Preceding gene: 117921243

Following gene: 117921241

Centisome position: 67.34

GC content: 53.02

Gene sequence:

>630_bases
ATGGCGGTATTTAAGACGTTGACCGACGCCGATATCGCTACCTTCTCTACAGGTTTAGTGGCGGGCGTGGATGAAGTCGG
TCGTGGCCCGCTGGTAGGGGATGTGGTCACGGCTGCGGTAATCCTCGATCCTAATCGCCCCATTGCAGGCTTAAATGATT
CTAAAAAGCTCAGCGAGAAGCGCCGCGAGGCCTTGTTCGATGAGATTTGTGAAAAAGCCTTGAGTTATCATGTGGGGAGG
GCCAGTCCTTTGGAAATCGATGAGCTGAATATCCTGCATGCCACAATGCTTGCGATGCAGCGTGCGGTAGCTGGGCTTGC
TCGTGCGCCCGAGTTAGTTTTAGTCGATGGTAATCGCAGCCCAGCGTTTACGCACCAGGGGCTGACTCTGATCAGTCATA
GCATAGTCAAAGGTGATGGCCTGATTGCGAGTATCAGTGCGGCGTCGATTATTGCCAAAGTCACCCGCGATCGGGAGATG
GATGCCCTCGATGCCGCTTATCCCCAGTATGGATTTGCTAAGCATAAGGGGTATCCGACTAAGGCGCATTTTGACGCCAT
CGCCGAGCACGGCGTGTTCGACCAATATCGTAAAAGTTTCAAACCCGTCAAGGCATTACTGGAGCGTTAA

Upstream 100 bases:

>100_bases
AAGCCGAGTTTGAGCGTTTACACCAAATGTTGCGCTGTGATGCCAGTCAAAAAGCGGCTGAGGCAGTGTTAGCGCTCGTC
GATGCTAAGGATGTGAACTA

Downstream 100 bases:

>100_bases
CACCGATTGGGTGTTTTCACTTCAGCTTGAGAGCAAAGTCACTAGCCCAAGGGCTGTGACTTTGTTAGTCTGCTTTTTTA
GTGATTGTTAACCTGCTATT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MAVFKTLTDADIATFSTGLVAGVDEVGRGPLVGDVVTAAVILDPNRPIAGLNDSKKLSEKRREALFDEICEKALSYHVGR
ASPLEIDELNILHATMLAMQRAVAGLARAPELVLVDGNRSPAFTHQGLTLISHSIVKGDGLIASISAASIIAKVTRDREM
DALDAAYPQYGFAKHKGYPTKAHFDAIAEHGVFDQYRKSFKPVKALLER

Sequences:

>Translated_209_residues
MAVFKTLTDADIATFSTGLVAGVDEVGRGPLVGDVVTAAVILDPNRPIAGLNDSKKLSEKRREALFDEICEKALSYHVGR
ASPLEIDELNILHATMLAMQRAVAGLARAPELVLVDGNRSPAFTHQGLTLISHSIVKGDGLIASISAASIIAKVTRDREM
DALDAAYPQYGFAKHKGYPTKAHFDAIAEHGVFDQYRKSFKPVKALLER
>Mature_208_residues
AVFKTLTDADIATFSTGLVAGVDEVGRGPLVGDVVTAAVILDPNRPIAGLNDSKKLSEKRREALFDEICEKALSYHVGRA
SPLEIDELNILHATMLAMQRAVAGLARAPELVLVDGNRSPAFTHQGLTLISHSIVKGDGLIASISAASIIAKVTRDREMD
ALDAAYPQYGFAKHKGYPTKAHFDAIAEHGVFDQYRKSFKPVKALLER

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=191, Percent_Identity=70.1570680628272, Blast_Score=261, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 22536; Mature: 22405

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFKTLTDADIATFSTGLVAGVDEVGRGPLVGDVVTAAVILDPNRPIAGLNDSKKLSEK
CCHHHHCCCCHHHHHHHHHHHCHHHHCCCCCHHHHHHEEEEECCCCCCCCCCCHHHHHHH
RREALFDEICEKALSYHVGRASPLEIDELNILHATMLAMQRAVAGLARAPELVLVDGNRS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
PAFTHQGLTLISHSIVKGDGLIASISAASIIAKVTRDREMDALDAAYPQYGFAKHKGYPT
CCHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHCCCCCC
KAHFDAIAEHGVFDQYRKSFKPVKALLER
HHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVFKTLTDADIATFSTGLVAGVDEVGRGPLVGDVVTAAVILDPNRPIAGLNDSKKLSEK
CHHHHCCCCHHHHHHHHHHHCHHHHCCCCCHHHHHHEEEEECCCCCCCCCCCHHHHHHH
RREALFDEICEKALSYHVGRASPLEIDELNILHATMLAMQRAVAGLARAPELVLVDGNRS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
PAFTHQGLTLISHSIVKGDGLIASISAASIIAKVTRDREMDALDAAYPQYGFAKHKGYPT
CCHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHCCCCCC
KAHFDAIAEHGVFDQYRKSFKPVKALLER
HHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]