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The map label for this gene is pleD [H]

Identifier: 117921239

GI number: 117921239

Start: 3341742

End: 3342632

Strand: Direct

Name: pleD [H]

Synonym: Shewana3_2798

Alternate gene names: 117921239

Gene position: 3341742-3342632 (Clockwise)

Preceding gene: 117921228

Following gene: 117921257

Centisome position: 67.21

GC content: 48.48

Gene sequence:

>891_bases
ATGGACTTTGGCTTAGCGACAACCTTATATCCAGACGATTATAACTATCAAACAGAGGCGTATCGCCCAACAACGTCGTC
ATTGGATTTAGTTCAAGTGATCCAGCAACTGCATGCCAGCCTAGATCCGCGGACAGTCTTTGCCTGCTACGGCAAAGTGC
TAGGACAACATCTGCCCGTCCAAGGTGTTCGCCTGCAAGCCGAGCAACAAAAATTAAGCTGGGGCAAACGTTATGGGATC
AGTCTGAAACGACAGATCATCTGTGGCGGCACCCCACTGACACTGCAGTATCAACTGCTGACGCCGCTCACACCTTCGCA
GACCATTATCCTGCAAGAGATTGAGCCCTTGCTGCTGCAACCATTATTAAATGCCATGCAGTATCAAGAGATGTCGATGC
AGGCGATGTTCGACTCCCTCACCGGCCTTGGCAATCGTCATTATTACACTCAAAGTTTGAAAAATGCGGTGGCCAGAGCC
CACAGAAAACAAGGCGCGGTATCACTTATCGTATTGGATTTGGATAATTTTAAGCAGCTTAACGATAGGTATGGTCACAA
ATGCGGTGATTATATTTTGAAGGAGTTTGGAGACATTATCCGTAGCAGTATCCGCAGTACAGATCAGGCCTTCCGTATCG
GAGGCGACGAGTTTGTGGTGATAGTGCAAGGCAATATTCATGCGGCAGGCTTACTGTGCGAACGTATCGTCACAGCGACC
AATGCCCATGCGAGTTTCCATCAATTTGGCGTAAGCAGTAGCTTAGGTGCGGCTGAGGCGAGCGATACAATGGAAGCCGA
ACAACTCTACGAGCAAGCGGATAAAACCTTGTATCAAGCCAAAGCCTCGGGTCGTAATTGCTACAAGTTAAGTCCAGCCC
AACTATCTTAA

Upstream 100 bases:

>100_bases
GACATACAAAGACAAATTTTATTAGCAAACAACACGGCACACATATTGCTTAATATGGACAATAGTTCCAATTGCCATTT
CATTTGCTGGGGTAAACATA

Downstream 100 bases:

>100_bases
TCGAGCATGGATGCTGCTCACTTGCGATAAAGCATGGCAAATACACTCTGCTATCCGATTGCTTGCCCATCAAACTTAAG
CCCGACATCATCCCCTTGGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDFGLATTLYPDDYNYQTEAYRPTTSSLDLVQVIQQLHASLDPRTVFACYGKVLGQHLPVQGVRLQAEQQKLSWGKRYGI
SLKRQIICGGTPLTLQYQLLTPLTPSQTIILQEIEPLLLQPLLNAMQYQEMSMQAMFDSLTGLGNRHYYTQSLKNAVARA
HRKQGAVSLIVLDLDNFKQLNDRYGHKCGDYILKEFGDIIRSSIRSTDQAFRIGGDEFVVIVQGNIHAAGLLCERIVTAT
NAHASFHQFGVSSSLGAAEASDTMEAEQLYEQADKTLYQAKASGRNCYKLSPAQLS

Sequences:

>Translated_296_residues
MDFGLATTLYPDDYNYQTEAYRPTTSSLDLVQVIQQLHASLDPRTVFACYGKVLGQHLPVQGVRLQAEQQKLSWGKRYGI
SLKRQIICGGTPLTLQYQLLTPLTPSQTIILQEIEPLLLQPLLNAMQYQEMSMQAMFDSLTGLGNRHYYTQSLKNAVARA
HRKQGAVSLIVLDLDNFKQLNDRYGHKCGDYILKEFGDIIRSSIRSTDQAFRIGGDEFVVIVQGNIHAAGLLCERIVTAT
NAHASFHQFGVSSSLGAAEASDTMEAEQLYEQADKTLYQAKASGRNCYKLSPAQLS
>Mature_296_residues
MDFGLATTLYPDDYNYQTEAYRPTTSSLDLVQVIQQLHASLDPRTVFACYGKVLGQHLPVQGVRLQAEQQKLSWGKRYGI
SLKRQIICGGTPLTLQYQLLTPLTPSQTIILQEIEPLLLQPLLNAMQYQEMSMQAMFDSLTGLGNRHYYTQSLKNAVARA
HRKQGAVSLIVLDLDNFKQLNDRYGHKCGDYILKEFGDIIRSSIRSTDQAFRIGGDEFVVIVQGNIHAAGLLCERIVTAT
NAHASFHQFGVSSSLGAAEASDTMEAEQLYEQADKTLYQAKASGRNCYKLSPAQLS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=183, Percent_Identity=32.7868852459016, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082007, Length=202, Percent_Identity=35.1485148514851, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081974, Length=199, Percent_Identity=32.6633165829146, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788381, Length=163, Percent_Identity=30.6748466257669, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787262, Length=157, Percent_Identity=34.3949044585987, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=30.188679245283, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=30, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=30.4347826086957, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=30.1775147928994, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787802, Length=167, Percent_Identity=31.1377245508982, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=33.5483870967742, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=207, Percent_Identity=28.9855072463768, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788956, Length=178, Percent_Identity=29.7752808988764, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=31.055900621118, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 32972; Mature: 32972

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFGLATTLYPDDYNYQTEAYRPTTSSLDLVQVIQQLHASLDPRTVFACYGKVLGQHLPV
CCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
QGVRLQAEQQKLSWGKRYGISLKRQIICGGTPLTLQYQLLTPLTPSQTIILQEIEPLLLQ
CCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCCCCEEHHHHHHHHHHH
PLLNAMQYQEMSMQAMFDSLTGLGNRHYYTQSLKNAVARAHRKQGAVSLIVLDLDNFKQL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHH
NDRYGHKCGDYILKEFGDIIRSSIRSTDQAFRIGGDEFVVIVQGNIHAAGLLCERIVTAT
HHHHCHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCEEEEEEECCCHHHHHHHHHHHHHC
NAHASFHQFGVSSSLGAAEASDTMEAEQLYEQADKTLYQAKASGRNCYKLSPAQLS
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MDFGLATTLYPDDYNYQTEAYRPTTSSLDLVQVIQQLHASLDPRTVFACYGKVLGQHLPV
CCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
QGVRLQAEQQKLSWGKRYGISLKRQIICGGTPLTLQYQLLTPLTPSQTIILQEIEPLLLQ
CCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCCCCEEHHHHHHHHHHH
PLLNAMQYQEMSMQAMFDSLTGLGNRHYYTQSLKNAVARAHRKQGAVSLIVLDLDNFKQL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHH
NDRYGHKCGDYILKEFGDIIRSSIRSTDQAFRIGGDEFVVIVQGNIHAAGLLCERIVTAT
HHHHCHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCEEEEEEECCCHHHHHHHHHHHHHC
NAHASFHQFGVSSSLGAAEASDTMEAEQLYEQADKTLYQAKASGRNCYKLSPAQLS
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA