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The map label for this gene is spsK [H]

Identifier: 117921234

GI number: 117921234

Start: 3332138

End: 3333232

Strand: Reverse

Name: spsK [H]

Synonym: Shewana3_2793

Alternate gene names: 117921234

Gene position: 3333232-3332138 (Counterclockwise)

Preceding gene: 117921235

Following gene: 117921233

Centisome position: 67.04

GC content: 51.78

Gene sequence:

>1095_bases
ATGTATGCGCCTAATGGCGAGCCAGAATCGACAGCAACAGTATTGTTGACTGGCGCAGATAGCCAACTGTCTAAGGCTCT
AGTGCGAGTGCTTGCCAGGGCGGCTTATCGATTCGATGGCCGCGCCTTTCGTGTGCACGCGCTTAGCCATGCCCAGTTAG
ATATTGGTGGTAAACAGAGCGTTGCCGCCGTCTTTGCCCACGTTAAACCTGACTGGGTGATCAACTGCGCCGCCTACAAT
GCGGTCGATAGGGCAGAAACGGCGGTGGATGAGGCTTATCGTGTCAATAGCCTTGGGCCAGAGTTATTGGCAAGCGAATG
TGCGCTCACGGGCGCGCGTTTGGTACATATCTCCAGCGATTATGTGTTTAGCGGCGAGCCAGCGGAGGTTGCTGAACTGG
CGTTAAATCAGAACAGCTTAACCGATAGCAGCTTAAGTAATAGTGGTTTAACTGAAAGCGGCTTAATTGAACGCGGGTTA
ACCGAAAGCGCCACACCAGCGCCCTTATCTGTCTATGGCAAGAGTAAACTCGCGGGGGAGCAAGCGGTGCTGCGCATCTT
GGCTGAGCGCGGGATGGTTATTCGCACCGCATGGCTGTACGGTGTCGATGGGCATAATTTTGTGAAAACCATGCTGAGGT
TGATGGCTACCATGCCCGATAGACAGCCACTCTCAGTGATAAACGACCAAATAGGTTCGCCGACATGGGCAGATGCACTC
GCGCATCTTATTTGGCAGTTAATAGCGAGTCCGTTAAACGCTAGTGGCGAACCCAGACTATTCCATTATGCTGGGCATGG
GCAATGCAGTTGGTATGAATTTGCCCTCGAAATCCAGCGCCAAGCCTTGGCACTGAAGTTATTAGATAAAGCGTTGCCGA
TTAGCGCCATAGATAGCCTAAGTTATGCTGCAAAAGCGCTTGAGAAAGGCAATAAACTGGCACCAAGGCCAAGTTACAGC
GCGCTAAATAGCGATAGAGTTCGCCAGTACTTAACTAGGGAGAATTTGGCATTAACCCATAGCCCCGAGCTGTGGCGTGA
CTGGCGGTGGCAGCTTAAGGCTATGCTTGAAGATTATGCCGAAAAATGCCCTTAG

Upstream 100 bases:

>100_bases
ACTCAGCGGGGGCATCAAAGCGTTGCTGATTTAATGCCGTTGTTTAGCGACGATATCGCTTGAGGCGCCGACGTTAATAA
TGCGAAAGGATAACAAGGCG

Downstream 100 bases:

>100_bases
CCATTATCTCTAACCATAAGTGGTTGTGAATGACGTTCAGTTCCTTTGAATACAAGATAAGTTGAATGCCCGAAGTGCTC
CACCCAAGCTACTGATTTAT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MYAPNGEPESTATVLLTGADSQLSKALVRVLARAAYRFDGRAFRVHALSHAQLDIGGKQSVAAVFAHVKPDWVINCAAYN
AVDRAETAVDEAYRVNSLGPELLASECALTGARLVHISSDYVFSGEPAEVAELALNQNSLTDSSLSNSGLTESGLIERGL
TESATPAPLSVYGKSKLAGEQAVLRILAERGMVIRTAWLYGVDGHNFVKTMLRLMATMPDRQPLSVINDQIGSPTWADAL
AHLIWQLIASPLNASGEPRLFHYAGHGQCSWYEFALEIQRQALALKLLDKALPISAIDSLSYAAKALEKGNKLAPRPSYS
ALNSDRVRQYLTRENLALTHSPELWRDWRWQLKAMLEDYAEKCP

Sequences:

>Translated_364_residues
MYAPNGEPESTATVLLTGADSQLSKALVRVLARAAYRFDGRAFRVHALSHAQLDIGGKQSVAAVFAHVKPDWVINCAAYN
AVDRAETAVDEAYRVNSLGPELLASECALTGARLVHISSDYVFSGEPAEVAELALNQNSLTDSSLSNSGLTESGLIERGL
TESATPAPLSVYGKSKLAGEQAVLRILAERGMVIRTAWLYGVDGHNFVKTMLRLMATMPDRQPLSVINDQIGSPTWADAL
AHLIWQLIASPLNASGEPRLFHYAGHGQCSWYEFALEIQRQALALKLLDKALPISAIDSLSYAAKALEKGNKLAPRPSYS
ALNSDRVRQYLTRENLALTHSPELWRDWRWQLKAMLEDYAEKCP
>Mature_364_residues
MYAPNGEPESTATVLLTGADSQLSKALVRVLARAAYRFDGRAFRVHALSHAQLDIGGKQSVAAVFAHVKPDWVINCAAYN
AVDRAETAVDEAYRVNSLGPELLASECALTGARLVHISSDYVFSGEPAEVAELALNQNSLTDSSLSNSGLTESGLIERGL
TESATPAPLSVYGKSKLAGEQAVLRILAERGMVIRTAWLYGVDGHNFVKTMLRLMATMPDRQPLSVINDQIGSPTWADAL
AHLIWQLIASPLNASGEPRLFHYAGHGQCSWYEFALEIQRQALALKLLDKALPISAIDSLSYAAKALEKGNKLAPRPSYS
ALNSDRVRQYLTRENLALTHSPELWRDWRWQLKAMLEDYAEKCP

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Escherichia coli, GI1788352, Length=348, Percent_Identity=31.3218390804598, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 39817; Mature: 39817

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAPNGEPESTATVLLTGADSQLSKALVRVLARAAYRFDGRAFRVHALSHAQLDIGGKQS
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEECCCCCHH
VAAVFAHVKPDWVINCAAYNAVDRAETAVDEAYRVNSLGPELLASECALTGARLVHISSD
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
YVFSGEPAEVAELALNQNSLTDSSLSNSGLTESGLIERGLTESATPAPLSVYGKSKLAGE
EEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCHHHHHH
QAVLRILAERGMVIRTAWLYGVDGHNFVKTMLRLMATMPDRQPLSVINDQIGSPTWADAL
HHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHH
AHLIWQLIASPLNASGEPRLFHYAGHGQCSWYEFALEIQRQALALKLLDKALPISAIDSL
HHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SYAAKALEKGNKLAPRPSYSALNSDRVRQYLTRENLALTHSPELWRDWRWQLKAMLEDYA
HHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHH
EKCP
HHCC
>Mature Secondary Structure
MYAPNGEPESTATVLLTGADSQLSKALVRVLARAAYRFDGRAFRVHALSHAQLDIGGKQS
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEECCCCCHH
VAAVFAHVKPDWVINCAAYNAVDRAETAVDEAYRVNSLGPELLASECALTGARLVHISSD
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
YVFSGEPAEVAELALNQNSLTDSSLSNSGLTESGLIERGLTESATPAPLSVYGKSKLAGE
EEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCHHHHHH
QAVLRILAERGMVIRTAWLYGVDGHNFVKTMLRLMATMPDRQPLSVINDQIGSPTWADAL
HHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHH
AHLIWQLIASPLNASGEPRLFHYAGHGQCSWYEFALEIQRQALALKLLDKALPISAIDSL
HHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SYAAKALEKGNKLAPRPSYSALNSDRVRQYLTRENLALTHSPELWRDWRWQLKAMLEDYA
HHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHH
EKCP
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]