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The map label for this gene is aprN [H]

Identifier: 117921186

GI number: 117921186

Start: 3275449

End: 3277014

Strand: Direct

Name: aprN [H]

Synonym: Shewana3_2745

Alternate gene names: 117921186

Gene position: 3275449-3277014 (Clockwise)

Preceding gene: 117921184

Following gene: 117921194

Centisome position: 65.88

GC content: 50.83

Gene sequence:

>1566_bases
ATGAAATTAACCAAAATCGCCAGTGCCGTTATTGCCTTATCTGTGGGCGTTATTGCCCAAGCCCATGCCGACGACAATCG
TTATGTCATTCAAGTCGAAGCGGGTCATAAAGGCATAGTTAAAGCACTGGCCAAAAAATTGGGCGCAGAAATCAACCTTG
AAGGGGATGAATTTATCGCTGCGACTTTTACCGGCAAAGATCTCGCCCAAGTAAAAGGCCTGCTAAACAACCCACATATC
ACCCTGATTGAAGAAGACGTTCGCCGTCATCCAATGGCGCTATATAACGACGATGCGGGCAATCCAATGACCCAGCAAGT
CACGCCCTATGCGATATATCAATCCCATGCCAACGAAGTAACTTTTAACCCAAATGCGGGCATGAAGGTGTGTATTATTG
ACTCGGGTTTAGATAGCTCAAACCCAGACTTTAACTGGAACAACATCACCGGCGATAACGACTCTGGTACGGGTAACTGG
TATCAGAACGGTGGCCCACACGGCACCCACGTAGCGGGTACTATAGGTGCGGCGGATAACAATATCGGTGTAGTCGGTAT
GGCGCCTGGCGTACCCATGCATATCGTAAAAGTATTCAACGCCTCGGGTTGGGGTTACTCCTCTGACTTAGCCTATGCAG
CCAACAAATGTAGTAATGCTGGCGCTAAGATCATCAGCATGAGTTTAGGTGGCGGCGCAGCCAACAATACTGAAAAGAAT
GCCTTCGACGCCTTTACTGCTGCGGGTGGTTTAGTGGTCGCGGCTGCGGGTAACGACGGTAACTCAGTGCGCTCCTATCC
AGCGGGTTACCCATCGGTGATGATGATCGGTGCTAACGATGCCAACAATAAGATTGCCGACTTCTCCCAATACCCAAGCT
GCGTGAGCGGTCGTGGTAAAAAAGCCGTCAACGATGATGGCATCTGTGTTGAAGTGACCGCAGGCGGTGTCGATACCCTA
TCAACCTACCCTGCAGGCATGGCAACGGCCTCAACCTTAACAGCCGATGGCGCGGCAGTCGCCTCATCAGCAATGGAAAA
CCCAGGTTCAGCCACGGGTAACACCTATTTTATGGGCACGGCTCAGGCAGTGGATTCGGGCGCGAACGGTAAGATTTGTG
TTATCGATCGCGGTGTGATTTCCTTCCACGATAAAGTGCTTAACTGTGAAAACTCAGGCGGTGTGGGCGCAATCGTTATC
AACAACGTGGCGGGCATGCTCTACGGCACTTTGGGTGATACCAACGCCACAACTATTCCTGCTGTTGGCGCAGCGTTTGA
AGATAGAGCGAGCCTACTTGCAGCCCAAAACGCAACGGTTTCAATCGATTCGGTTGACTACGGCTTTATGAGCGGTACTT
CAATGGCCACACCAGCAGTATCGGGTATGGCGGCATTGGTATGGTCTAACCATTCACAATGTACCGGCACACAAATCCGT
AACGCACTCAAAGCAACGGCAATGGATGCGGGTACTGTAGGTAAGGATAACTACTTCGGTTATGGTATCGTCAACGCGAA
AGCGGCCGATGCTTACCTCACTACCTATGGCTGCTCTGGTAAATAG

Upstream 100 bases:

>100_bases
ATCGCCTAAGTCAGCAGACGCCCGTCTGTCCATAGGAGCACAAGTAGATGCTCCGTGTCGCTTAGGTCTTACAACCTTGA
TAACAAATGGAGCAAGCACA

Downstream 100 bases:

>100_bases
TATTTAGCCAAGATAATCCCAGTAAAAAAACCAGCACTTGTGCTGGTTTTTTTATTGTCTATCGGAAGATTACTTAAACC
AACCTTTGATACTGCTCAGC

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MKLTKIASAVIALSVGVIAQAHADDNRYVIQVEAGHKGIVKALAKKLGAEINLEGDEFIAATFTGKDLAQVKGLLNNPHI
TLIEEDVRRHPMALYNDDAGNPMTQQVTPYAIYQSHANEVTFNPNAGMKVCIIDSGLDSSNPDFNWNNITGDNDSGTGNW
YQNGGPHGTHVAGTIGAADNNIGVVGMAPGVPMHIVKVFNASGWGYSSDLAYAANKCSNAGAKIISMSLGGGAANNTEKN
AFDAFTAAGGLVVAAAGNDGNSVRSYPAGYPSVMMIGANDANNKIADFSQYPSCVSGRGKKAVNDDGICVEVTAGGVDTL
STYPAGMATASTLTADGAAVASSAMENPGSATGNTYFMGTAQAVDSGANGKICVIDRGVISFHDKVLNCENSGGVGAIVI
NNVAGMLYGTLGDTNATTIPAVGAAFEDRASLLAAQNATVSIDSVDYGFMSGTSMATPAVSGMAALVWSNHSQCTGTQIR
NALKATAMDAGTVGKDNYFGYGIVNAKAADAYLTTYGCSGK

Sequences:

>Translated_521_residues
MKLTKIASAVIALSVGVIAQAHADDNRYVIQVEAGHKGIVKALAKKLGAEINLEGDEFIAATFTGKDLAQVKGLLNNPHI
TLIEEDVRRHPMALYNDDAGNPMTQQVTPYAIYQSHANEVTFNPNAGMKVCIIDSGLDSSNPDFNWNNITGDNDSGTGNW
YQNGGPHGTHVAGTIGAADNNIGVVGMAPGVPMHIVKVFNASGWGYSSDLAYAANKCSNAGAKIISMSLGGGAANNTEKN
AFDAFTAAGGLVVAAAGNDGNSVRSYPAGYPSVMMIGANDANNKIADFSQYPSCVSGRGKKAVNDDGICVEVTAGGVDTL
STYPAGMATASTLTADGAAVASSAMENPGSATGNTYFMGTAQAVDSGANGKICVIDRGVISFHDKVLNCENSGGVGAIVI
NNVAGMLYGTLGDTNATTIPAVGAAFEDRASLLAAQNATVSIDSVDYGFMSGTSMATPAVSGMAALVWSNHSQCTGTQIR
NALKATAMDAGTVGKDNYFGYGIVNAKAADAYLTTYGCSGK
>Mature_521_residues
MKLTKIASAVIALSVGVIAQAHADDNRYVIQVEAGHKGIVKALAKKLGAEINLEGDEFIAATFTGKDLAQVKGLLNNPHI
TLIEEDVRRHPMALYNDDAGNPMTQQVTPYAIYQSHANEVTFNPNAGMKVCIIDSGLDSSNPDFNWNNITGDNDSGTGNW
YQNGGPHGTHVAGTIGAADNNIGVVGMAPGVPMHIVKVFNASGWGYSSDLAYAANKCSNAGAKIISMSLGGGAANNTEKN
AFDAFTAAGGLVVAAAGNDGNSVRSYPAGYPSVMMIGANDANNKIADFSQYPSCVSGRGKKAVNDDGICVEVTAGGVDTL
STYPAGMATASTLTADGAAVASSAMENPGSATGNTYFMGTAQAVDSGANGKICVIDRGVISFHDKVLNCENSGGVGAIVI
NNVAGMLYGTLGDTNATTIPAVGAAFEDRASLLAAQNATVSIDSVDYGFMSGTSMATPAVSGMAALVWSNHSQCTGTQIR
NALKATAMDAGTVGKDNYFGYGIVNAKAADAYLTTYGCSGK

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 53256; Mature: 53256

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKIASAVIALSVGVIAQAHADDNRYVIQVEAGHKGIVKALAKKLGAEINLEGDEFIA
CCHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHHHHHHHHHCCCEEEECCCCEEE
ATFTGKDLAQVKGLLNNPHITLIEEDVRRHPMALYNDDAGNPMTQQVTPYAIYQSHANEV
EEECCCHHHHHHHHHCCCCEEEEECHHHHCCCEEEECCCCCCCHHCCCCEEEEECCCCEE
TFNPNAGMKVCIIDSGLDSSNPDFNWNNITGDNDSGTGNWYQNGGPHGTHVAGTIGAADN
EECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
NIGVVGMAPGVPMHIVKVFNASGWGYSSDLAYAANKCSNAGAKIISMSLGGGAANNTEKN
CEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
AFDAFTAAGGLVVAAAGNDGNSVRSYPAGYPSVMMIGANDANNKIADFSQYPSCVSGRGK
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHCCHHHCCCCC
KAVNDDGICVEVTAGGVDTLSTYPAGMATASTLTADGAAVASSAMENPGSATGNTYFMGT
CCCCCCCEEEEEECCCCCCHHCCCCCCCCHHEECCCCHHHHHHHHCCCCCCCCCEEEEEE
AQAVDSGANGKICVIDRGVISFHDKVLNCENSGGVGAIVINNVAGMLYGTLGDTNATTIP
HHHHCCCCCCEEEEEECCHHHCCHHEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCCC
AVGAAFEDRASLLAAQNATVSIDSVDYGFMSGTSMATPAVSGMAALVWSNHSQCTGTQIR
CCCCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCCCHHCCEEEEEECCCCCCCHHHHH
NALKATAMDAGTVGKDNYFGYGIVNAKAADAYLTTYGCSGK
HHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCC
>Mature Secondary Structure
MKLTKIASAVIALSVGVIAQAHADDNRYVIQVEAGHKGIVKALAKKLGAEINLEGDEFIA
CCHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHHHHHHHHHCCCEEEECCCCEEE
ATFTGKDLAQVKGLLNNPHITLIEEDVRRHPMALYNDDAGNPMTQQVTPYAIYQSHANEV
EEECCCHHHHHHHHHCCCCEEEEECHHHHCCCEEEECCCCCCCHHCCCCEEEEECCCCEE
TFNPNAGMKVCIIDSGLDSSNPDFNWNNITGDNDSGTGNWYQNGGPHGTHVAGTIGAADN
EECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
NIGVVGMAPGVPMHIVKVFNASGWGYSSDLAYAANKCSNAGAKIISMSLGGGAANNTEKN
CEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
AFDAFTAAGGLVVAAAGNDGNSVRSYPAGYPSVMMIGANDANNKIADFSQYPSCVSGRGK
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHCCHHHCCCCC
KAVNDDGICVEVTAGGVDTLSTYPAGMATASTLTADGAAVASSAMENPGSATGNTYFMGT
CCCCCCCEEEEEECCCCCCHHCCCCCCCCHHEECCCCHHHHHHHHCCCCCCCCCEEEEEE
AQAVDSGANGKICVIDRGVISFHDKVLNCENSGGVGAIVINNVAGMLYGTLGDTNATTIP
HHHHCCCCCCEEEEEECCHHHCCHHEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCCC
AVGAAFEDRASLLAAQNATVSIDSVDYGFMSGTSMATPAVSGMAALVWSNHSQCTGTQIR
CCCCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCCCHHCCEEEEEECCCCCCCHHHHH
NALKATAMDAGTVGKDNYFGYGIVNAKAADAYLTTYGCSGK
HHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]