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The map label for this gene is dhaAF [H]

Identifier: 117921155

GI number: 117921155

Start: 3244479

End: 3245441

Strand: Reverse

Name: dhaAF [H]

Synonym: Shewana3_2714

Alternate gene names: 117921155

Gene position: 3245441-3244479 (Counterclockwise)

Preceding gene: 117921156

Following gene: 117921154

Centisome position: 65.27

GC content: 49.53

Gene sequence:

>963_bases
ATGTTAGACACTCTGTTGCCGTTTAAACGTCATTTTCTTAGCCGTAATGGCAACAAGCTGCATTACATCAATGAAGGCCA
AGGTGAGCCTGTCGTCATGGTGCACGGTAATCCAAGTTGGAGTTTTTATTACCGTAATCTCGTGAGTGCCCTTAAGGATA
CTCACCAGTGTATCGTGCCCGATCATATTGGTTGTGGCTTGTCGGATAAGCCGGATGACAGCGGTTACGATTACACCCTT
AAGAATCGCATTGATGATTTAGAGGCGCTGCTCGACAGTCTAAAGGTAAAAGAAAACATTACCTTAGTCGTCCACGACTG
GGGCGGCATGATAGGCATGGGCTATGCGGCGCGTTACCCTGAGCGCATTAAACGTCTGGTGATTTTAAATACTGGCGCCT
TCCATTTACCCGAGACTAAGCCTTTACCGCTGGCGCTGTGGATCTGCCGCAATACGCTACTCGGCACAGTATTAGTGCGT
GGTTTTAATGCCTTTTCATCGATTGCCTCCTACGTTGGGGTAAAGCGTCAGCCGATGTCCAAGTACATCCGTGAGGCCTA
CGTCGCGCCGTTTAACTCATGGGCAAATCGGATTTCGACCCTGCGTTTTGTGCAGGATATTCCGCTAAAACCGGGTGACA
GAAACTATCAACTGGTGTCGGATATCGCCGCCAGTCTGCCAAAGTTTGCCAAGGTGCCGACACTGATTTGCTGGGGTTTA
CAGGACTTTGTGTTCGATAAACACTTTTTAGCCAAATGGCGCGAACATATGCCCCATGCACAAGTGCATGAATTTGCCGA
TTGTGGTCACTATATCCTCGAAGATGCGAGCGATGAGGTGATTACCCATATCAAACACTTTATGGCTGAGGCGCAAACGC
CTGAGCCAGAAGTACAGCCCGTTGAAACGGAACAGGTCGCAGAGCCTGAGGCAGAGACTCAAGCGCCACAGGCCGCACAT
TAA

Upstream 100 bases:

>100_bases
TGGTTTAGCGATGGGCATTAGCGATGAGCAGAGGTAAAGGCGAATTTACCTGCACAGGCTGCCAATACAAAAACAATTAG
AACAAAAGATAGGAAGCGTC

Downstream 100 bases:

>100_bases
TGGCTAGGGTGGAGGATGCGCAGCTCGGCCATGGGACATCTAGCGTCGAGCAAGCAAGCGATGCCAATATTTGTCGGCAT
TTAAAGCAGGCCGCCCATCA

Product: alpha/beta hydrolase fold domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MLDTLLPFKRHFLSRNGNKLHYINEGQGEPVVMVHGNPSWSFYYRNLVSALKDTHQCIVPDHIGCGLSDKPDDSGYDYTL
KNRIDDLEALLDSLKVKENITLVVHDWGGMIGMGYAARYPERIKRLVILNTGAFHLPETKPLPLALWICRNTLLGTVLVR
GFNAFSSIASYVGVKRQPMSKYIREAYVAPFNSWANRISTLRFVQDIPLKPGDRNYQLVSDIAASLPKFAKVPTLICWGL
QDFVFDKHFLAKWREHMPHAQVHEFADCGHYILEDASDEVITHIKHFMAEAQTPEPEVQPVETEQVAEPEAETQAPQAAH

Sequences:

>Translated_320_residues
MLDTLLPFKRHFLSRNGNKLHYINEGQGEPVVMVHGNPSWSFYYRNLVSALKDTHQCIVPDHIGCGLSDKPDDSGYDYTL
KNRIDDLEALLDSLKVKENITLVVHDWGGMIGMGYAARYPERIKRLVILNTGAFHLPETKPLPLALWICRNTLLGTVLVR
GFNAFSSIASYVGVKRQPMSKYIREAYVAPFNSWANRISTLRFVQDIPLKPGDRNYQLVSDIAASLPKFAKVPTLICWGL
QDFVFDKHFLAKWREHMPHAQVHEFADCGHYILEDASDEVITHIKHFMAEAQTPEPEVQPVETEQVAEPEAETQAPQAAH
>Mature_320_residues
MLDTLLPFKRHFLSRNGNKLHYINEGQGEPVVMVHGNPSWSFYYRNLVSALKDTHQCIVPDHIGCGLSDKPDDSGYDYTL
KNRIDDLEALLDSLKVKENITLVVHDWGGMIGMGYAARYPERIKRLVILNTGAFHLPETKPLPLALWICRNTLLGTVLVR
GFNAFSSIASYVGVKRQPMSKYIREAYVAPFNSWANRISTLRFVQDIPLKPGDRNYQLVSDIAASLPKFAKVPTLICWGL
QDFVFDKHFLAKWREHMPHAQVHEFADCGHYILEDASDEVITHIKHFMAEAQTPEPEVQPVETEQVAEPEAETQAPQAAH

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Caenorhabditis elegans, GI17562298, Length=131, Percent_Identity=31.2977099236641, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554012, Length=114, Percent_Identity=29.8245614035088, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 36305; Mature: 36305

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTLLPFKRHFLSRNGNKLHYINEGQGEPVVMVHGNPSWSFYYRNLVSALKDTHQCIVP
CCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEECC
DHIGCGLSDKPDDSGYDYTLKNRIDDLEALLDSLKVKENITLVVHDWGGMIGMGYAARYP
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCHHHHCH
ERIKRLVILNTGAFHLPETKPLPLALWICRNTLLGTVLVRGFNAFSSIASYVGVKRQPMS
HHHCEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KYIREAYVAPFNSWANRISTLRFVQDIPLKPGDRNYQLVSDIAASLPKFAKVPTLICWGL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHCCCHHHHHHH
QDFVFDKHFLAKWREHMPHAQVHEFADCGHYILEDASDEVITHIKHFMAEAQTPEPEVQP
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
VETEQVAEPEAETQAPQAAH
CCHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MLDTLLPFKRHFLSRNGNKLHYINEGQGEPVVMVHGNPSWSFYYRNLVSALKDTHQCIVP
CCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEECC
DHIGCGLSDKPDDSGYDYTLKNRIDDLEALLDSLKVKENITLVVHDWGGMIGMGYAARYP
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCHHHHCH
ERIKRLVILNTGAFHLPETKPLPLALWICRNTLLGTVLVRGFNAFSSIASYVGVKRQPMS
HHHCEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KYIREAYVAPFNSWANRISTLRFVQDIPLKPGDRNYQLVSDIAASLPKFAKVPTLICWGL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHCCCHHHHHHH
QDFVFDKHFLAKWREHMPHAQVHEFADCGHYILEDASDEVITHIKHFMAEAQTPEPEVQP
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
VETEQVAEPEAETQAPQAAH
CCHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]