Click here to switch to the map view.

The map label for this gene is pspA [H]

Identifier: 117921089

GI number: 117921089

Start: 3161232

End: 3161915

Strand: Reverse

Name: pspA [H]

Synonym: Shewana3_2648

Alternate gene names: 117921089

Gene position: 3161915-3161232 (Counterclockwise)

Preceding gene: 117921098

Following gene: 117921088

Centisome position: 63.59

GC content: 43.42

Gene sequence:

>684_bases
ATGGGAATTTTCTCTCGTTTCGCCGATATCATTAACTCCAATATCAGCGCATTACTCGATAAAGCAGAAGACCCTGAAAA
AATGGTTCGTCTGATTATCCAAGAGATGGAAGATACACTGGTAGAAGTTCGTTCAACATCAGCAAAAGTGTTAGCTGAAA
AGAAAGAATTAATCCGTCGTATCAGCCGTGTTGAAGAACAAGTTCAAGATTGGCAAGACAAAGCTGAGCTGGCACTGTCG
AAGGATCGCGAAGATTTAGCCAAGGCCGCTTTAGTTGAAAAACAAAAAGCCGCGGGTTTAGTGGATACCTTAAACCAAGA
GTTGGCAGTGATCGACGAGCACATTGTGCGCTTAAAGGATGAAGTTAGCCTGCTGCAAGAAAAGTTACTGGATGCTAAGG
CGCGCCAAAAGACCATTATTTTGCGTAAACAAACCGCCTCATCACGTTTAGAAGTGAAGAAGCAGTTAGACTCAAGCAAA
ATCGACAACGCTATGCTGAAATTTGAGCAATATGAGCGCCGTGTCGAAGGGTTAGAAGCGCAGGTTGAATCCTACGATCT
GGGTAATAAAAAGACCTTAGCCGATGAGTTTGCTGCACTGGAAGCTGAAGATTCAGTGAATGCCGAGCTGGAAGCATTAA
AGGCCAAAGTAAAAGGCAAAGCTGCGACTAAGTCAAAAGAATAA

Upstream 100 bases:

>100_bases
TAAGAATATGTTTATCTTTATGAATTTAAACATAAAATAAAAGTTGGCACACTTAGTGTATTACCCTAAGCGAGACCAAC
ATCAATTTGAGGATAGCATT

Downstream 100 bases:

>100_bases
CAATTCAGAGGTGAGTTATGGATATGGACATACTAATGGCACCAATTATCATCTTTATGATAGTGGTGGCGCCCATATGG
TTAGTTCTTCATTATCGCAG

Product: phage shock protein A, PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELIRRISRVEEQVQDWQDKAELALS
KDREDLAKAALVEKQKAAGLVDTLNQELAVIDEHIVRLKDEVSLLQEKLLDAKARQKTIILRKQTASSRLEVKKQLDSSK
IDNAMLKFEQYERRVEGLEAQVESYDLGNKKTLADEFAALEAEDSVNAELEALKAKVKGKAATKSKE

Sequences:

>Translated_227_residues
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELIRRISRVEEQVQDWQDKAELALS
KDREDLAKAALVEKQKAAGLVDTLNQELAVIDEHIVRLKDEVSLLQEKLLDAKARQKTIILRKQTASSRLEVKKQLDSSK
IDNAMLKFEQYERRVEGLEAQVESYDLGNKKTLADEFAALEAEDSVNAELEALKAKVKGKAATKSKE
>Mature_226_residues
GIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELIRRISRVEEQVQDWQDKAELALSK
DREDLAKAALVEKQKAAGLVDTLNQELAVIDEHIVRLKDEVSLLQEKLLDAKARQKTIILRKQTASSRLEVKKQLDSSKI
DNAMLKFEQYERRVEGLEAQVESYDLGNKKTLADEFAALEAEDSVNAELEALKAKVKGKAATKSKE

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=218, Percent_Identity=55.9633027522936, Blast_Score=249, Evalue=9e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25608; Mature: 25476

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELIRR
CCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISRVEEQVQDWQDKAELALSKDREDLAKAALVEKQKAAGLVDTLNQELAVIDEHIVRLKD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVSLLQEKLLDAKARQKTIILRKQTASSRLEVKKQLDSSKIDNAMLKFEQYERRVEGLEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVESYDLGNKKTLADEFAALEAEDSVNAELEALKAKVKGKAATKSKE
HHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELIRR
CHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISRVEEQVQDWQDKAELALSKDREDLAKAALVEKQKAAGLVDTLNQELAVIDEHIVRLKD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVSLLQEKLLDAKARQKTIILRKQTASSRLEVKKQLDSSKIDNAMLKFEQYERRVEGLEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVESYDLGNKKTLADEFAALEAEDSVNAELEALKAKVKGKAATKSKE
HHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]