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The map label for this gene is cspLB [H]

Identifier: 117921010

GI number: 117921010

Start: 3073707

End: 3073916

Strand: Reverse

Name: cspLB [H]

Synonym: Shewana3_2569

Alternate gene names: 117921010

Gene position: 3073916-3073707 (Counterclockwise)

Preceding gene: 117921011

Following gene: 117921007

Centisome position: 61.82

GC content: 42.86

Gene sequence:

>210_bases
ATGTCTCAAGTTACTGGTGTTGTTAAGTGGTTCAACTCTGACAAAGGTTTCGGCTTCATCGAGCAAGAATCTGGTCCAGA
CGTATTCGTACACTTCCGTGCAATCAACTCTGACGGTTTCAAAACTCTTGACGAAGGTCAAAAAGTGTCTTTCACTGTGA
CTCAAGGTCAAAAAGGTCCTCAAGCTGAAAACGTAACCGTAATCGGTTAA

Upstream 100 bases:

>100_bases
GTTCAATACGACCAGTTCGTCCTGTAAACATAAGTAATACCGGCATTTTCCAGCTCCACCGCCGTTAAGGCTTTAATTTT
TTATTTACAGGATTTATATC

Downstream 100 bases:

>100_bases
TTTTCAGTGCTGTTAGCCTAGCGCTGATAGCAATGGATTTAAGAGCTGTGGTTGATACCATGGCTCTTTTTATTTGTGTA
GCTTTTAAGTTTTTACACTC

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: CspB [H]

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MSQVTGVVKWFNSDKGFGFIEQESGPDVFVHFRAINSDGFKTLDEGQKVSFTVTQGQKGPQAENVTVIG

Sequences:

>Translated_69_residues
MSQVTGVVKWFNSDKGFGFIEQESGPDVFVHFRAINSDGFKTLDEGQKVSFTVTQGQKGPQAENVTVIG
>Mature_68_residues
SQVTGVVKWFNSDKGFGFIEQESGPDVFVHFRAINSDGFKTLDEGQKVSFTVTQGQKGPQAENVTVIG

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=65.6716417910448, Blast_Score=92, Evalue=4e-21,
Organism=Escherichia coli, GI1786841, Length=69, Percent_Identity=60.8695652173913, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI2367114, Length=68, Percent_Identity=58.8235294117647, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=58.2089552238806, Blast_Score=83, Evalue=4e-18,
Organism=Escherichia coli, GI1788126, Length=69, Percent_Identity=60.8695652173913, Blast_Score=82, Evalue=7e-18,
Organism=Escherichia coli, GI1787834, Length=68, Percent_Identity=54.4117647058823, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=50, Blast_Score=64, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7523; Mature: 7392

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVTGVVKWFNSDKGFGFIEQESGPDVFVHFRAINSDGFKTLDEGQKVSFTVTQGQKGP
CCCCCEEEEEECCCCCCEEEECCCCCEEEEEEEEECCCCCEECCCCCEEEEEEECCCCCC
QAENVTVIG
CCCCEEEEC
>Mature Secondary Structure 
SQVTGVVKWFNSDKGFGFIEQESGPDVFVHFRAINSDGFKTLDEGQKVSFTVTQGQKGP
CCCCEEEEEECCCCCCEEEECCCCCEEEEEEEEECCCCCEECCCCCEEEEEEECCCCCC
QAENVTVIG
CCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]