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The map label for this gene is garR [C]

Identifier: 117920995

GI number: 117920995

Start: 3056179

End: 3057054

Strand: Reverse

Name: garR [C]

Synonym: Shewana3_2554

Alternate gene names: 117920995

Gene position: 3057054-3056179 (Counterclockwise)

Preceding gene: 117920996

Following gene: 117920993

Centisome position: 61.48

GC content: 51.6

Gene sequence:

>876_bases
ATGGCAAAAGTCGCATTTATTGGTTTAGGCGTAATGGGTTACCCCATGGCAAGACACTTACTCAACAAGGGCCATGAGGT
TACTGTGTATAACCGCACTTTTGCCAAGGCACAAACCTGGGTCGAGAACTACGGCGGTCGTTGCTGCCCAACGCCTAAAG
AAGCGGCTATTGGCCAAGATATTGTTTTTACCTGCGTAGGCAACGATAACGACTTGCGTGAAGTGGTATTAGGCAATGAT
GGCGTGGTCCATGGCATGGCGCCAGGTACAGTACTAGTTGACCATACGACGGCTTCTGCCGATGTGGCCCGTGAATTACA
TAAAGTGCTGGCCGAAAAAGGCGTCGATTTTCTCGATGCGCCTGTATCGGGTGGCCAAGCTGGCGCCGAAAATGGCGTAC
TGACCGTGATGGTCGGGGGTGATCAAGCCGTATTCGAGCGTGTTAAGCCCGTTATCGAAGCGTTTGCGCGCTGCGCAGAG
CGTTTAGGTGAGGTTGGTGCAGGCCAGCTCACTAAGATGGTCAACCAAATTTGTATCGCAGGTGTGGTTCAAGGCCTTGC
CGAAGCACTGCAATTTGCCCGTAAGGCTGGTCTTGACGGTGAAAAAGTGGTTGAGGTGATCAGTAAAGGCGCGGCGCAAA
GCTGGCAGATGGAAAATCGCTACAAAACCATGTGGGGCCAAAGCTATGATTTTGGTTTTGCCGTCGATTGGATGCGTAAG
GATTTAGGCATTGCCCTAGAAGAAGCTCGCCGCAATGGCTCGCACTTACCCTTAACCGCGCTAGTGGATCAGTTTTACTC
CGAAGTTCAAGCCATGGGCGGCAATCGTTGGGATACCTCAAGCTTGTTAGCGCGTTTTGAGAAAAGCACTAAGTAG

Upstream 100 bases:

>100_bases
GTTGTCAATTCAGTGTTTTCAATAGAGTGATGCTAATGAACGCAGTACAAATGTGTGGTGTAAAACGTTTCACCTTGTTT
ATCAATTAAAGGAATTCCTC

Downstream 100 bases:

>100_bases
GTTGTTGCCTAATAGATGGCCATCATAAACTTGATGGCATAAAAAAGCCCAGTCATGCACTGGGCTTTTTCACTGATGGG
GTGATAAAGACCGATTAGTC

Product: 6-phosphogluconate dehydrogenase, NAD-binding

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVENYGGRCCPTPKEAAIGQDIVFTCVGNDNDLREVVLGND
GVVHGMAPGTVLVDHTTASADVARELHKVLAEKGVDFLDAPVSGGQAGAENGVLTVMVGGDQAVFERVKPVIEAFARCAE
RLGEVGAGQLTKMVNQICIAGVVQGLAEALQFARKAGLDGEKVVEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
DLGIALEEARRNGSHLPLTALVDQFYSEVQAMGGNRWDTSSLLARFEKSTK

Sequences:

>Translated_291_residues
MAKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVENYGGRCCPTPKEAAIGQDIVFTCVGNDNDLREVVLGND
GVVHGMAPGTVLVDHTTASADVARELHKVLAEKGVDFLDAPVSGGQAGAENGVLTVMVGGDQAVFERVKPVIEAFARCAE
RLGEVGAGQLTKMVNQICIAGVVQGLAEALQFARKAGLDGEKVVEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
DLGIALEEARRNGSHLPLTALVDQFYSEVQAMGGNRWDTSSLLARFEKSTK
>Mature_290_residues
AKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVENYGGRCCPTPKEAAIGQDIVFTCVGNDNDLREVVLGNDG
VVHGMAPGTVLVDHTTASADVARELHKVLAEKGVDFLDAPVSGGQAGAENGVLTVMVGGDQAVFERVKPVIEAFARCAER
LGEVGAGQLTKMVNQICIAGVVQGLAEALQFARKAGLDGEKVVEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRKD
LGIALEEARRNGSHLPLTALVDQFYSEVQAMGGNRWDTSSLLARFEKSTK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=29.2857142857143, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI23308751, Length=288, Percent_Identity=30.5555555555556, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=37.7162629757785, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=35.7894736842105, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=30.2721088435374, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789092, Length=302, Percent_Identity=33.112582781457, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1788341, Length=302, Percent_Identity=26.158940397351, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=28, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24655230, Length=282, Percent_Identity=31.5602836879433, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922568, Length=282, Percent_Identity=31.5602836879433, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=24.9110320284698, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24639279, Length=225, Percent_Identity=28.8888888888889, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31424; Mature: 31293

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVENYGGRCCPTPKEAAIGQD
CCEEEEECCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
IVFTCVGNDNDLREVVLGNDGVVHGMAPGTVLVDHTTASADVARELHKVLAEKGVDFLDA
EEEEEECCCCCHHHHEECCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEECC
PVSGGQAGAENGVLTVMVGGDQAVFERVKPVIEAFARCAERLGEVGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVVQGLAEALQFARKAGLDGEKVVEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DLGIALEEARRNGSHLPLTALVDQFYSEVQAMGGNRWDTSSLLARFEKSTK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVENYGGRCCPTPKEAAIGQD
CEEEEECCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
IVFTCVGNDNDLREVVLGNDGVVHGMAPGTVLVDHTTASADVARELHKVLAEKGVDFLDA
EEEEEECCCCCHHHHEECCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEECC
PVSGGQAGAENGVLTVMVGGDQAVFERVKPVIEAFARCAERLGEVGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVVQGLAEALQFARKAGLDGEKVVEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DLGIALEEARRNGSHLPLTALVDQFYSEVQAMGGNRWDTSSLLARFEKSTK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA