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The map label for this gene is pepE [H]

Identifier: 117920956

GI number: 117920956

Start: 3003376

End: 3004086

Strand: Direct

Name: pepE [H]

Synonym: Shewana3_2513

Alternate gene names: 117920956

Gene position: 3003376-3004086 (Clockwise)

Preceding gene: 117920953

Following gene: 117920957

Centisome position: 60.4

GC content: 49.51

Gene sequence:

>711_bases
ATGACAATCAATGCCCTACTGCTGAGCAGCTCTCGCGTCGGTGATACGCCTTACCTTTCCCATGCGCTTCCCTTTATCAA
GCCGCTCACCGCTAACGCCCAAAAATGGATTTTTATCCCTTATGCTGGTGTCAGTATGAGTTATGACACTTATTTGGCCT
CAGTCGTGGCGGGATTAAGCGAACTTAGGCTCGATATCAGCGGCATTCACCAGCATCCTGATCCGCGCCAAGCGATTAAA
GATGCCGACGGCATTTTGATCGGTGGCGGCAATACCTTCCATTTACTGCACGAGCTCTATAAGTATGATTTAGTGCATCT
TATCCGTGAAGAAGTGCAGAATGGTAAACCCTATATTGGTTGGAGCGCGGGCTCTAATGTCTCCGGATTGAGTATTCGCA
CCACCAACGATATGCCGATTATTGAGCCACCGTCTTTTACCGCGTTAAATATCGTGCCGTTTCAGTTAAATCCACATTAT
TCGAATTATCGCGCTCCGGGACATAACGGCGAAACCCGCGCCCAACGTTTGCTCGAATTCACTCGTGTCGATCCCATCAC
GCCGGTTGTGGGTATTGTCGAAGGCAGCGCACTTTGGCGTCAGGGAGACACCTTATCTCTGCTAGGTGACAATCCGGCCT
ATTTATTCTGCGGTGAGCAGCAAGAAATCCCGATCCCTGTCGGCAGCGATTTATCCCATTTGCTGAAATAA

Upstream 100 bases:

>100_bases
GCTGTCAGCGTTACAGCCAAATTCATGTGTGTTTATCTATGTTTATCGCGATTAAACTTTGTTAACATGCCAGCACATTC
CGAGATTTCAGGATAAAAAA

Downstream 100 bases:

>100_bases
GATCTATGCGGCGGCATTGCCTAAGCGGATCTGCTACTATCGGCCACCGAGCATGAGTGTGATGAGTGTGTCATCAAGCT
GGCTGGCCGATAACATGCCG

Product: peptidase E

Products: NA

Alternate protein names: Alpha-aspartyl dipeptidase; Asp-specific dipeptidase; Dipeptidase E [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTINALLLSSSRVGDTPYLSHALPFIKPLTANAQKWIFIPYAGVSMSYDTYLASVVAGLSELRLDISGIHQHPDPRQAIK
DADGILIGGGNTFHLLHELYKYDLVHLIREEVQNGKPYIGWSAGSNVSGLSIRTTNDMPIIEPPSFTALNIVPFQLNPHY
SNYRAPGHNGETRAQRLLEFTRVDPITPVVGIVEGSALWRQGDTLSLLGDNPAYLFCGEQQEIPIPVGSDLSHLLK

Sequences:

>Translated_236_residues
MTINALLLSSSRVGDTPYLSHALPFIKPLTANAQKWIFIPYAGVSMSYDTYLASVVAGLSELRLDISGIHQHPDPRQAIK
DADGILIGGGNTFHLLHELYKYDLVHLIREEVQNGKPYIGWSAGSNVSGLSIRTTNDMPIIEPPSFTALNIVPFQLNPHY
SNYRAPGHNGETRAQRLLEFTRVDPITPVVGIVEGSALWRQGDTLSLLGDNPAYLFCGEQQEIPIPVGSDLSHLLK
>Mature_235_residues
TINALLLSSSRVGDTPYLSHALPFIKPLTANAQKWIFIPYAGVSMSYDTYLASVVAGLSELRLDISGIHQHPDPRQAIKD
ADGILIGGGNTFHLLHELYKYDLVHLIREEVQNGKPYIGWSAGSNVSGLSIRTTNDMPIIEPPSFTALNIVPFQLNPHYS
NYRAPGHNGETRAQRLLEFTRVDPITPVVGIVEGSALWRQGDTLSLLGDNPAYLFCGEQQEIPIPVGSDLSHLLK

Specific function: Hydrolyzes dipeptides containing N-terminal aspartate residues. May play a role in allowing the cell to use peptide aspartate to spare carbon otherwise required for the synthesis of the aspartate family of amino acids [H]

COG id: COG3340

COG function: function code E; Peptidase E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S51 family [H]

Homologues:

Organism=Escherichia coli, GI1790452, Length=223, Percent_Identity=41.2556053811659, Blast_Score=184, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24641669, Length=223, Percent_Identity=43.9461883408072, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005320
- InterPro:   IPR023172 [H]

Pfam domain/function: PF03575 Peptidase_S51 [H]

EC number: =3.4.13.21 [H]

Molecular weight: Translated: 25925; Mature: 25794

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINALLLSSSRVGDTPYLSHALPFIKPLTANAQKWIFIPYAGVSMSYDTYLASVVAGLS
CEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ELRLDISGIHQHPDPRQAIKDADGILIGGGNTFHLLHELYKYDLVHLIREEVQNGKPYIG
HHHHHHHHCCCCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WSAGSNVSGLSIRTTNDMPIIEPPSFTALNIVPFQLNPHYSNYRAPGHNGETRAQRLLEF
ECCCCCCCCEEEEECCCCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
TRVDPITPVVGIVEGSALWRQGDTLSLLGDNPAYLFCGEQQEIPIPVGSDLSHLLK
HCCCCCCCEEEEECCCHHEECCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
TINALLLSSSRVGDTPYLSHALPFIKPLTANAQKWIFIPYAGVSMSYDTYLASVVAGLS
EEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ELRLDISGIHQHPDPRQAIKDADGILIGGGNTFHLLHELYKYDLVHLIREEVQNGKPYIG
HHHHHHHHCCCCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WSAGSNVSGLSIRTTNDMPIIEPPSFTALNIVPFQLNPHYSNYRAPGHNGETRAQRLLEF
ECCCCCCCCEEEEECCCCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
TRVDPITPVVGIVEGSALWRQGDTLSLLGDNPAYLFCGEQQEIPIPVGSDLSHLLK
HCCCCCCCEEEEECCCHHEECCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]