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The map label for this gene is ycdT [H]

Identifier: 117920926

GI number: 117920926

Start: 2969437

End: 2970840

Strand: Reverse

Name: ycdT [H]

Synonym: Shewana3_2483

Alternate gene names: 117920926

Gene position: 2970840-2969437 (Counterclockwise)

Preceding gene: 117920927

Following gene: 117920923

Centisome position: 59.75

GC content: 49.07

Gene sequence:

>1404_bases
TTGAAGACACTTTTTTCGCCTTTATTGCCAGCAAGACAAAGCCATATCGCACTGCTGTGCGCAGGCATTATCTACTGGCT
AACCGGCGCCTTAGGCCAAACATTATTTAGCTTACAACCTGAGAATATCACCTTGATTTGGCTGCCCTCCGGTATTGCCT
TGGTGATGTTACTTATGTGGGGGCGTAAGGCATTGCTGCTGATATTCCTCGTCAGTTTTACCTTGAATTTTCAAGGCATG
TTGCAACAGGATTCCCTCGGTCTTTCGCTGCTGCATACTTCGATTGCCGCGTTAAGCGATATGCTCGCGCCTGCGATGTC
GATGTTGTTACTCAGACGCTTCTTGCCACAGGGTACGGGAAGTGCGAATGACTTGATGAAGTTTGTGGTGGTCGCAGGCA
TCATCCCCATTTTCGGTTCCAGCGCATTGTTATCCGCTAATTTAGTGCTTGGCGGTTATATTTTACCCAGCGCGTTTTGG
AGTATGGGGCAAATGTTTTTCTTTGCCGATATTCTGGGAATAGTATTGGTGTATCAGCTGTATGCAGGATGGGTGGAACC
CAATGCGCTAAGCATAGCCAAAACCCGCCATATCCTTTTGCCCTTGATTGGCTTGCCACTGTTTTGTTTGGTGGGGGTTA
AGTTCGATCTGGGCTGGTTATTTTATGTGATCCCGCCGCTGTTGGTTGTGCTCTCCTTTGAGGTCACGCGTTTTTACCTC
GCGCTGTTTAGCAGCGCCTCCATGCTGTTTATGATTTTAGCGACAGCTCAGGGTTATGGTCCGTTTATCAATGCCACGCC
ATATCAAACCAATGCGGAGATGATGGCTTTTGTGTTGTCATCGGCATTAACGATTTTTGGGGTGTCGTTACAGCGGGCAC
AGCTGCGCCGAACCGAGAGAGATAAGCAAACTGCGGTGCAAGAGGCGCTCTATGATCCCTTGTCGGGGCTACTGAATCGC
CGCGGCTTTATGCCACAGCTGACAAATCTTAACGGCCAGACAGTGCGTTATTCGGTCGCTATGTTGGATCTCGATAACTT
TAAGATGATTAACGACACCTATGGTGACAGTGTGGGCGATAGGGTTATTCAGCTGTTGGCGCAGTTGATACACAATCATA
GCCGTAGCCAAGATATTGCCGCGCGCCTAGGCGGTGAAGAGTTCGCCCTTGTGCTTAGGGATATTGATGCTGAGCAGGTG
TATCCGGTGCTTGAGCGTATTCGAAAATCCTTTGCTGAGATGTCGATAGCGGCCGAGGAGCACACGATTTACTGCACTGT
GAGCATTGGTTGGGTGGAGTATCAGCAAGGTATCGCTGGCGAGTCGTTGCTGCATTTGGCGGATAAGGCTTTGTATCAAG
CCAAGGCAAAGGGCAAAAACACGATTGTAAAGTATCAAGCTTGA

Upstream 100 bases:

>100_bases
TTGGTTGTACTGCCATTAATGCACATGGCATAAGCTTTAATAGCGAAACAATAAACCGTTTAATTTTATCGCGTTAGCGC
TCTTTGAGGTTGATTACCGA

Downstream 100 bases:

>100_bases
GAGCCATGCCAAGAGGCTCCGTTCGAATTAGCAATGCTCAAGCGTACCGTCAAGGGGCGATACTCAAAAGGCCATGTTGA
AAGAAAATAATCGAAAGGCC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKTLFSPLLPARQSHIALLCAGIIYWLTGALGQTLFSLQPENITLIWLPSGIALVMLLMWGRKALLLIFLVSFTLNFQGM
LQQDSLGLSLLHTSIAALSDMLAPAMSMLLLRRFLPQGTGSANDLMKFVVVAGIIPIFGSSALLSANLVLGGYILPSAFW
SMGQMFFFADILGIVLVYQLYAGWVEPNALSIAKTRHILLPLIGLPLFCLVGVKFDLGWLFYVIPPLLVVLSFEVTRFYL
ALFSSASMLFMILATAQGYGPFINATPYQTNAEMMAFVLSSALTIFGVSLQRAQLRRTERDKQTAVQEALYDPLSGLLNR
RGFMPQLTNLNGQTVRYSVAMLDLDNFKMINDTYGDSVGDRVIQLLAQLIHNHSRSQDIAARLGGEEFALVLRDIDAEQV
YPVLERIRKSFAEMSIAAEEHTIYCTVSIGWVEYQQGIAGESLLHLADKALYQAKAKGKNTIVKYQA

Sequences:

>Translated_467_residues
MKTLFSPLLPARQSHIALLCAGIIYWLTGALGQTLFSLQPENITLIWLPSGIALVMLLMWGRKALLLIFLVSFTLNFQGM
LQQDSLGLSLLHTSIAALSDMLAPAMSMLLLRRFLPQGTGSANDLMKFVVVAGIIPIFGSSALLSANLVLGGYILPSAFW
SMGQMFFFADILGIVLVYQLYAGWVEPNALSIAKTRHILLPLIGLPLFCLVGVKFDLGWLFYVIPPLLVVLSFEVTRFYL
ALFSSASMLFMILATAQGYGPFINATPYQTNAEMMAFVLSSALTIFGVSLQRAQLRRTERDKQTAVQEALYDPLSGLLNR
RGFMPQLTNLNGQTVRYSVAMLDLDNFKMINDTYGDSVGDRVIQLLAQLIHNHSRSQDIAARLGGEEFALVLRDIDAEQV
YPVLERIRKSFAEMSIAAEEHTIYCTVSIGWVEYQQGIAGESLLHLADKALYQAKAKGKNTIVKYQA
>Mature_467_residues
MKTLFSPLLPARQSHIALLCAGIIYWLTGALGQTLFSLQPENITLIWLPSGIALVMLLMWGRKALLLIFLVSFTLNFQGM
LQQDSLGLSLLHTSIAALSDMLAPAMSMLLLRRFLPQGTGSANDLMKFVVVAGIIPIFGSSALLSANLVLGGYILPSAFW
SMGQMFFFADILGIVLVYQLYAGWVEPNALSIAKTRHILLPLIGLPLFCLVGVKFDLGWLFYVIPPLLVVLSFEVTRFYL
ALFSSASMLFMILATAQGYGPFINATPYQTNAEMMAFVLSSALTIFGVSLQRAQLRRTERDKQTAVQEALYDPLSGLLNR
RGFMPQLTNLNGQTVRYSVAMLDLDNFKMINDTYGDSVGDRVIQLLAQLIHNHSRSQDIAARLGGEEFALVLRDIDAEQV
YPVLERIRKSFAEMSIAAEEHTIYCTVSIGWVEYQQGIAGESLLHLADKALYQAKAKGKNTIVKYQA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=157, Percent_Identity=39.4904458598726, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=33.1395348837209, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788956, Length=180, Percent_Identity=35, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=36.875, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=36.9047619047619, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=30.8139534883721, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787802, Length=168, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=299, Percent_Identity=27.0903010033445, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786584, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081977, Length=161, Percent_Identity=29.8136645962733, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787816, Length=153, Percent_Identity=31.3725490196078, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081974, Length=121, Percent_Identity=34.7107438016529, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51653; Mature: 51653

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure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EEEEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]