Click here to switch to the map view.

The map label for this gene is rimK [H]

Identifier: 117920848

GI number: 117920848

Start: 2882821

End: 2883837

Strand: Direct

Name: rimK [H]

Synonym: Shewana3_2405

Alternate gene names: 117920848

Gene position: 2882821-2883837 (Clockwise)

Preceding gene: 117920847

Following gene: 117920849

Centisome position: 57.98

GC content: 47.49

Gene sequence:

>1017_bases
ATGCGTGGGTGGATTTTATATAAAGAAACCGCGACTCAGCTAAAACCTGAATTGTATGAAATTGAACGTCTATTGGCAGC
AGCCAAGGCCGACAATATTGAGTTAGAAGTGTATGCTCCGGATGAGTTCGATCTCACCGTGACGAGAGAAGACAACAAGA
GTATTTTGTTAAACGGCCAGCCCGTTGAATTACCCGATTTTATCATCCCGAGAATGGGCTCCGGCACCACTTACTTTGCG
TTGGCGATCATTCGCCATTTAGAACGCTTAGGGGTTTACTGCTTAAATCCATCTAAAGCCATTGAAATCGTTAAAGATAA
GCTCTTTTCCCAGCAATTACTGGCCGAGAAGAATTTACCCACGCCCAAAACCATGTTGGTGAAGTTCCCCGTCGATATCG
AACTGGTTGAACGTCACTTAGGTTTTCCCGTGGTGATTAAAACCCTCTCGGGCTCTCAGGGTAGCGGCGTATTTTTGTCC
CATAAGGCCCGCGAATTTGATGATTTAATGCAATTAATCGAAGCGACCAATAAAAACGCCAACATTATTTTGCAGGAGTT
TATCGCCAACAGCCACGGCCGCGATCTCAGGGTATTTACTATTGGTGGACGCGTAGCCGGCTGCTATGAACGCCGTGGGC
AAGAAGACAGCTTTAAAGCAAATGTCAGCGCGGGAGGTTCGGCGCGCCCCTTCGAGATCACACCTGAAATCGAATGGCTA
GCGACACAAACCGCCAATATTCTTGATTTAGATGTTGCAGGTATCGATTTGTTATTCGATAACGGCCACTACAAAATCTG
TGAAGCCAACTCATCCCCCGGTTTCGAAGGCTTAGAATCCTGCTTAAACGTGGATATTGCTGCGCAAATCCTGCACTTTA
TCCGCATCCGTTTAGGCATGTTTAACAAGGACGAGAAAAGTAGCGAGTCAACGCCTGCTCAAATGGCAGCGCCAGAGGTG
AGTTCCGCGACGAATACCAGCGCCTCGACACCTACCGCTAACACGCATAGCAAGTGA

Upstream 100 bases:

>100_bases
AGCCCGAAGCGGCATTCGATGTGGATGTCGAAATTGACGATGATGTTCAGGATGAAGCTGAACCCGAGGATAAAGAATGA
AAATGAAGGTTTGCGATTAA

Downstream 100 bases:

>100_bases
TGCTGCATCCTTAAGCTCTTATCTGCGGCCCAGTCTTTACTGGGCCGCATTTTTTGGCCTGACTATACATTTTCGTCACA
AATCACTGGGATGCAAATTT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MRGWILYKETATQLKPELYEIERLLAAAKADNIELEVYAPDEFDLTVTREDNKSILLNGQPVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIELVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFEITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDEKSSESTPAQMAAPEV
SSATNTSASTPTANTHSK

Sequences:

>Translated_338_residues
MRGWILYKETATQLKPELYEIERLLAAAKADNIELEVYAPDEFDLTVTREDNKSILLNGQPVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIELVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFEITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDEKSSESTPAQMAAPEV
SSATNTSASTPTANTHSK
>Mature_338_residues
MRGWILYKETATQLKPELYEIERLLAAAKADNIELEVYAPDEFDLTVTREDNKSILLNGQPVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIELVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFEITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDEKSSESTPAQMAAPEV
SSATNTSASTPTANTHSK

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI134254462, Length=342, Percent_Identity=27.4853801169591, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI164663810, Length=298, Percent_Identity=27.8523489932886, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1787076, Length=278, Percent_Identity=34.5323741007194, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 37385; Mature: 37385

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGWILYKETATQLKPELYEIERLLAAAKADNIELEVYAPDEFDLTVTREDNKSILLNGQ
CCCEEEECCHHHHHCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEECCC
PVELPDFIIPRMGSGTTYFALAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLP
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
TPKTMLVKFPVDIELVERHLGFPVVIKTLSGSQGSGVFLSHKAREFDDLMQLIEATNKNA
CCCEEEEECCCCHHHHHHHCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
NIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFEITPEIEWL
CEEHHHHHCCCCCCEEEEEEECCCEEHHHHHCCCCCCEEEECCCCCCCCCEEECCCCEEE
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGM
EECCCCEEEEEECCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
FNKDEKSSESTPAQMAAPEVSSATNTSASTPTANTHSK
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRGWILYKETATQLKPELYEIERLLAAAKADNIELEVYAPDEFDLTVTREDNKSILLNGQ
CCCEEEECCHHHHHCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEECCC
PVELPDFIIPRMGSGTTYFALAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLP
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
TPKTMLVKFPVDIELVERHLGFPVVIKTLSGSQGSGVFLSHKAREFDDLMQLIEATNKNA
CCCEEEEECCCCHHHHHHHCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
NIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFEITPEIEWL
CEEHHHHHCCCCCCEEEEEEECCCEEHHHHHCCCCCCEEEECCCCCCCCCEEECCCCEEE
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGM
EECCCCEEEEEECCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
FNKDEKSSESTPAQMAAPEVSSATNTSASTPTANTHSK
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA