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The map label for this gene is 117920837

Identifier: 117920837

GI number: 117920837

Start: 2873231

End: 2874070

Strand: Direct

Name: 117920837

Synonym: Shewana3_2394

Alternate gene names: NA

Gene position: 2873231-2874070 (Clockwise)

Preceding gene: 117920835

Following gene: 117920838

Centisome position: 57.79

GC content: 48.81

Gene sequence:

>840_bases
ATGAAATTAGAAACCATTGATTATCGCGCAGCCGATAGCGCCAAACGCTTTGTCGAATCCCTACGTGAAACGGGCTTTGG
TGTGCTCTCTAACCACCCTATCGATAAAGAGCTGGTCGAAAGCATTTACGCTGAGTGGCAAGCCTTTTTCAATTCTGACG
CTAAGAACGAGTTTATGTTCAATCGTGAAACCCACGATGGCTTCTTCCCCGCGTCGATTTCTGAAACCGCAAAAGGCCAC
ACGGTTAAAGACATCAAAGAGTATTACCACGTGTACACTTGGGGTCGTATTCCAGATTCTTTGCGTGCCAATATTCTGGC
CTACTATGAAAAAGCCAATGCGCTGGCCTCTGAACTGTTAGAGTGGATTGAAACCTACTCGCCAGACGAGGTTAAAGCCA
AATTCTCTATCCCGCTGCCAGAAATGATTGCCGACAGCCACAAAACCCTGCTGCGCATTCTGCATTACCCACCGATGACG
GGCGATGAAGAAATGGGCGCAATCCGCGCTGCCGCCCACGAAGATATTAACCTTATCACTGTACTGCCTGCGGCCAACGA
ACCAGGGCTGCAAGTCAAAGCCAAAGACGGCTCTTGGTTAGATGTGCCAAGTGATTTTGGCAATATTATCATCAACATTG
GTGATATGTTGCAGGAAGCCTCAGGCGGTTACTTCCCATCCACCTCACACCGCGTGATTAACCCAGAAGGCACGGATAAG
ACTAAGTCGCGTATTTCTCTGCCCTTATTCTTACACCCGAATCCTTCGGTCGTGCTGTCTGAGCGTTATACCGCCGACAG
CTACTTAATGGAAAGATTGCGCGAACTGGGCGTACTTTAA

Upstream 100 bases:

>100_bases
TTAGCGCACCATACCGATAATGAGATAAGTGGCAATGACTCCTTACCTTTATCCTTAGAAAGTGTTTGGTTAACAACCAT
ATATAACAAGTAGAAACAAT

Downstream 100 bases:

>100_bases
CTCAGCCCCAAGCAATAGATGCTCAAATTCGCATCAAGATTAAGCAATTAAAGCGCCTTCGGGCGCTTTTTTTATGACAT
TCGTCGCCTTCAAGGTAGAA

Product: 2OG-Fe(II) oxygenase

Products: isopenicillin N; H2O

Alternate protein names: Oxidoreductase; 2OG-Fe(II) Oxygenase Family Oxidoreductase; Oxidoreductase 2OG-Fe(II) Oxygenase Family; Isopenicillin N Synthase; Iron/Ascorbate Family Oxidoreductase; 2OG-Fe(II) Oxygenase Superfamily Protein; Oxidoreductase 2OG-Fe(II) Oxygenase Family Protein; Flavonol Synthase/Dioxygenase; 2og-Fe(Ii) Oxygenase; Oxidoreductase Iron/Ascorbate Family Protein; Iron/Ascorbate Oxidoreductase; 1-Aminocyclopropane-1-Carboxylate Oxidase; Oxygenase; Isopenicillin N Synthase And Related Dioxygenases; Oxidoreductase 2og-Fe(Ii) Oxygenase Family; Iron/Ascorbate Dependent Oxidoreductase; Iron/Ascorbate-Dependent Oxidoreductase Family Protein; Iron/Ascorbate-Dependent Oxidoreductase; Oxidoreductase Iron/Ascorbate Family; 2OG-Fe(II) Oxygenase Family Protein; Fe-Containing Oxidoreductase; Isopenicillin-N Synthase; Flavonol Synthase/Flavanone 3-Hydroxylase; 2-Oxoglutarate And Fe(Ii)-Dependent Oxygenase; Oxygenase/Oxidoreductase; Iron/Ascorbate Oxidoreductase Family Protein

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKLETIDYRAADSAKRFVESLRETGFGVLSNHPIDKELVESIYAEWQAFFNSDAKNEFMFNRETHDGFFPASISETAKGH
TVKDIKEYYHVYTWGRIPDSLRANILAYYEKANALASELLEWIETYSPDEVKAKFSIPLPEMIADSHKTLLRILHYPPMT
GDEEMGAIRAAAHEDINLITVLPAANEPGLQVKAKDGSWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
TKSRISLPLFLHPNPSVVLSERYTADSYLMERLRELGVL

Sequences:

>Translated_279_residues
MKLETIDYRAADSAKRFVESLRETGFGVLSNHPIDKELVESIYAEWQAFFNSDAKNEFMFNRETHDGFFPASISETAKGH
TVKDIKEYYHVYTWGRIPDSLRANILAYYEKANALASELLEWIETYSPDEVKAKFSIPLPEMIADSHKTLLRILHYPPMT
GDEEMGAIRAAAHEDINLITVLPAANEPGLQVKAKDGSWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
TKSRISLPLFLHPNPSVVLSERYTADSYLMERLRELGVL
>Mature_279_residues
MKLETIDYRAADSAKRFVESLRETGFGVLSNHPIDKELVESIYAEWQAFFNSDAKNEFMFNRETHDGFFPASISETAKGH
TVKDIKEYYHVYTWGRIPDSLRANILAYYEKANALASELLEWIETYSPDEVKAKFSIPLPEMIADSHKTLLRILHYPPMT
GDEEMGAIRAAAHEDINLITVLPAANEPGLQVKAKDGSWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
TKSRISLPLFLHPNPSVVLSERYTADSYLMERLRELGVL

Specific function: Unknown

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Drosophila melanogaster, GI28572124, Length=267, Percent_Identity=26.5917602996255, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI85816264, Length=273, Percent_Identity=25.2747252747253, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.21.3.1

Molecular weight: Translated: 31472; Mature: 31472

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLETIDYRAADSAKRFVESLRETGFGVLSNHPIDKELVESIYAEWQAFFNSDAKNEFMF
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
NRETHDGFFPASISETAKGHTVKDIKEYYHVYTWGRIPDSLRANILAYYEKANALASELL
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EWIETYSPDEVKAKFSIPLPEMIADSHKTLLRILHYPPMTGDEEMGAIRAAAHEDINLIT
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
VLPAANEPGLQVKAKDGSWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
EEECCCCCCCEEEECCCCEEECCCCCCCEEEEHHHHHHHHCCCCCCCCCCEEECCCCCCC
TKSRISLPLFLHPNPSVVLSERYTADSYLMERLRELGVL
HHHHEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLETIDYRAADSAKRFVESLRETGFGVLSNHPIDKELVESIYAEWQAFFNSDAKNEFMF
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
NRETHDGFFPASISETAKGHTVKDIKEYYHVYTWGRIPDSLRANILAYYEKANALASELL
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EWIETYSPDEVKAKFSIPLPEMIADSHKTLLRILHYPPMTGDEEMGAIRAAAHEDINLIT
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
VLPAANEPGLQVKAKDGSWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
EEECCCCCCCEEEECCCCEEECCCCCCCEEEEHHHHHHHHCCCCCCCCCCEEECCCCCCC
TKSRISLPLFLHPNPSVVLSERYTADSYLMERLRELGVL
HHHHEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine; O2

Specific reaction: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA