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The map label for this gene is malS [C]

Identifier: 117920756

GI number: 117920756

Start: 2783465

End: 2785525

Strand: Direct

Name: malS [C]

Synonym: Shewana3_2313

Alternate gene names: 117920756

Gene position: 2783465-2785525 (Clockwise)

Preceding gene: 117920755

Following gene: 117920757

Centisome position: 55.98

GC content: 48.52

Gene sequence:

>2061_bases
ATGATACGCCGCTCTTTATTTCTAAAGTCACACTTAACACCTCACCCGATAAAGGCCCATAGTATGATACGTCACAGTCT
ACTCTTCGCCCTTGTGTTAACCGCCTCTTCTCCCTTATATGCTCTAGCACAGGCCAGTCATAGCCCCGTCAGCAGTACAG
CATCTTTGCATCTCGAGCCGCTCTCTTGGTGGGCGGGCATGCATAATCCTAAGCTGCAATTAATGCTCCACAGTGATGCC
CTGCCCACGGATGCTGCGCAGCTTAAGGTTGAGATTACTGGCCAAGATGTGTTGTTTGAAGGGATTGAACAGACGGATAA
TCCCCATTATTTGTTTATCAATTTAGACTTGCGCCAAGCCAAACCCCAGACCTTTGATATCCGCATTCTCGCTGGCGATA
AGCAGCTCTTACAACTGCCCTACACTTTAGCGACACGGGCAGAAGGTAGCCGCGAACGCCAAGGCTTCAACAATAAAGAT
GTAATTTACCTTATCACTCCAGACAGATTCGCCAATGGCAACCCAAGCAATGACAATCAGAGCGATATGCTCGAGCATGC
GGCGCCTGATAATTCCGATGGTCGCCATGGCGGCGATATCGAAGGTATACGCCAGCACTTAGACTACTTAGCTAAACTGG
GCGTCACTCAGTTGTGGATCAACCCACTGCTTGAAAACAACCAAGCCCAATACTCCTACCATGGTTACGCCATGACCAAT
CTTTATCGGGTCGACCCGCGTTTTGGCAGTAATCAGGATTATCAAAGGTTAGTCACAGAGGCCAAACAGCATGGTCTTGG
GGTGATTAAAGACGTGGTGCTTAACCATATCGGCTCAAACCATTGGTGGCTAAAAGATTTACCGAGCAGTGACTGGCTCA
ATGTCCCGCAGCAGCCCAATGCAACTGACACCCAAACGGCTCCTGCGGCCAGTTCTACCAGCCTCAGTTCTACCAACCTG
GGCTTTACCAGCCATAGACGCACCACAGTGCAAGATCCCTACGCCGACATGCAGGATAAAAGAGATTTTGTTGACGGTTG
GTTTGTCGATTCGATGCCCGATCTTAATCAACGCAACCCAAAGCTTGCCACTTATCTTATTCAAAACAGCATTTGGTGGG
TGGAATACGCGGGGCTTTCGGGCATTCGTGAAGACACCTACTCCTACGCCGATAAAGCCTTTTTAACCCAATGGACTAAG
GCATTAATGGCAGAGTACCCACATTTTAATATTGTCGGTGAAGAGTGGACCGCCAATCCCATAACTGTCTCCTATTGGCA
AAAAGGCAAAATCAATGCCGATGGCTATAGCTCGGATTTACCGAGCCTGATGGACTTTCCACTCTATGAAAAGCTGCTTG
AAAGCCTGAACGAGCCTGAAGGCTGGGATACAGGTTTTATCAAGCTTTACGAGATGTTAGCTAATGATGTGGTGTATGCG
ACCCCAAGCCAGTTAGTCCTCTTTGAAGGCAACCACGATACCAATCGGATTTATAGTCTGCTGAAAGAAAACCTGCCGCT
GTACCAAATGGCCCTCACCTATGTGTTAACGGCCAAACGCATTCCACAGCTGTTTTACGGTACCGAAGTGTTGATGACCA
GCCCGACCGAAGGCCGCCACGACGGTGTAGTGCGTAGCGATTTTCCGGGTGGCTTTGCCAATAACAAGGTCAACGGTTTT
AGCGGTAAAGGGCTAAACGCAAAACAGCAGCAAGCGCTTGCGTTTGTGCGTACTCTACTCAATTACCGTAAGCATTCAAC
CGCACTGCAAACAGGCGATTTACTGCACTTTGTGCCGCAAAACGGCATTTATGTGCAATTTCGATGCGTTGCCAAACAGG
CTGCAGCGGGCAATGCCTGCTACCAAGCCGATACGGATAAAGTGATGGTGATTTACAACAAGAATGACCAAGCACAAACC
CTGGATTTAAGCCGCTTTAGCAAGGTATTGGCAGGCAATACTTCGGCTAACTCAGTCCTCTCTGGTGATAAGTTAAAGCT
GAATCAATCACTTGTTCTGCCCCATGCTGGCGTTATGCTGCTTGAACTCAATCATCAATAA

Upstream 100 bases:

>100_bases
ACAATATTTCCTTGGTTTAACCTACTCTCTGTAAGCTGTCATCGTAAAAGCCTGTTTCACTGTGAAGCAGGCTTTTCTTT
TTACCCATAATAATAGTGAG

Downstream 100 bases:

>100_bases
CCCATATTCAATTAAGCAGCTAAGCATGCTTAGCTAACAGCGCGGCCAAACCATAGTTTGGCCGCCAAGTCAGTAAAGGA
ACCTAGGTTGAATTTTCTAT

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: CDase; Cyclomaltodextrin hydrolase, decycling [H]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MIRRSLFLKSHLTPHPIKAHSMIRHSLLFALVLTASSPLYALAQASHSPVSSTASLHLEPLSWWAGMHNPKLQLMLHSDA
LPTDAAQLKVEITGQDVLFEGIEQTDNPHYLFINLDLRQAKPQTFDIRILAGDKQLLQLPYTLATRAEGSRERQGFNNKD
VIYLITPDRFANGNPSNDNQSDMLEHAAPDNSDGRHGGDIEGIRQHLDYLAKLGVTQLWINPLLENNQAQYSYHGYAMTN
LYRVDPRFGSNQDYQRLVTEAKQHGLGVIKDVVLNHIGSNHWWLKDLPSSDWLNVPQQPNATDTQTAPAASSTSLSSTNL
GFTSHRRTTVQDPYADMQDKRDFVDGWFVDSMPDLNQRNPKLATYLIQNSIWWVEYAGLSGIREDTYSYADKAFLTQWTK
ALMAEYPHFNIVGEEWTANPITVSYWQKGKINADGYSSDLPSLMDFPLYEKLLESLNEPEGWDTGFIKLYEMLANDVVYA
TPSQLVLFEGNHDTNRIYSLLKENLPLYQMALTYVLTAKRIPQLFYGTEVLMTSPTEGRHDGVVRSDFPGGFANNKVNGF
SGKGLNAKQQQALAFVRTLLNYRKHSTALQTGDLLHFVPQNGIYVQFRCVAKQAAAGNACYQADTDKVMVIYNKNDQAQT
LDLSRFSKVLAGNTSANSVLSGDKLKLNQSLVLPHAGVMLLELNHQ

Sequences:

>Translated_686_residues
MIRRSLFLKSHLTPHPIKAHSMIRHSLLFALVLTASSPLYALAQASHSPVSSTASLHLEPLSWWAGMHNPKLQLMLHSDA
LPTDAAQLKVEITGQDVLFEGIEQTDNPHYLFINLDLRQAKPQTFDIRILAGDKQLLQLPYTLATRAEGSRERQGFNNKD
VIYLITPDRFANGNPSNDNQSDMLEHAAPDNSDGRHGGDIEGIRQHLDYLAKLGVTQLWINPLLENNQAQYSYHGYAMTN
LYRVDPRFGSNQDYQRLVTEAKQHGLGVIKDVVLNHIGSNHWWLKDLPSSDWLNVPQQPNATDTQTAPAASSTSLSSTNL
GFTSHRRTTVQDPYADMQDKRDFVDGWFVDSMPDLNQRNPKLATYLIQNSIWWVEYAGLSGIREDTYSYADKAFLTQWTK
ALMAEYPHFNIVGEEWTANPITVSYWQKGKINADGYSSDLPSLMDFPLYEKLLESLNEPEGWDTGFIKLYEMLANDVVYA
TPSQLVLFEGNHDTNRIYSLLKENLPLYQMALTYVLTAKRIPQLFYGTEVLMTSPTEGRHDGVVRSDFPGGFANNKVNGF
SGKGLNAKQQQALAFVRTLLNYRKHSTALQTGDLLHFVPQNGIYVQFRCVAKQAAAGNACYQADTDKVMVIYNKNDQAQT
LDLSRFSKVLAGNTSANSVLSGDKLKLNQSLVLPHAGVMLLELNHQ
>Mature_686_residues
MIRRSLFLKSHLTPHPIKAHSMIRHSLLFALVLTASSPLYALAQASHSPVSSTASLHLEPLSWWAGMHNPKLQLMLHSDA
LPTDAAQLKVEITGQDVLFEGIEQTDNPHYLFINLDLRQAKPQTFDIRILAGDKQLLQLPYTLATRAEGSRERQGFNNKD
VIYLITPDRFANGNPSNDNQSDMLEHAAPDNSDGRHGGDIEGIRQHLDYLAKLGVTQLWINPLLENNQAQYSYHGYAMTN
LYRVDPRFGSNQDYQRLVTEAKQHGLGVIKDVVLNHIGSNHWWLKDLPSSDWLNVPQQPNATDTQTAPAASSTSLSSTNL
GFTSHRRTTVQDPYADMQDKRDFVDGWFVDSMPDLNQRNPKLATYLIQNSIWWVEYAGLSGIREDTYSYADKAFLTQWTK
ALMAEYPHFNIVGEEWTANPITVSYWQKGKINADGYSSDLPSLMDFPLYEKLLESLNEPEGWDTGFIKLYEMLANDVVYA
TPSQLVLFEGNHDTNRIYSLLKENLPLYQMALTYVLTAKRIPQLFYGTEVLMTSPTEGRHDGVVRSDFPGGFANNKVNGF
SGKGLNAKQQQALAFVRTLLNYRKHSTALQTGDLLHFVPQNGIYVQFRCVAKQAAAGNACYQADTDKVMVIYNKNDQAQT
LDLSRFSKVLAGNTSANSVLSGDKLKLNQSLVLPHAGVMLLELNHQ

Specific function: Hydrolyzes cyclodextrins. Can also act on linear maltodextrins, with the exception of maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=129, Percent_Identity=36.4341085271318, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790687, Length=202, Percent_Identity=27.2277227722772, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786604, Length=196, Percent_Identity=28.0612244897959, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322245, Length=124, Percent_Identity=34.6774193548387, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6322241, Length=211, Percent_Identity=27.4881516587678, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322021, Length=211, Percent_Identity=27.4881516587678, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321726, Length=211, Percent_Identity=27.0142180094787, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324416, Length=211, Percent_Identity=27.0142180094787, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321731, Length=203, Percent_Identity=26.6009852216749, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=203, Percent_Identity=26.6009852216749, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24586591, Length=206, Percent_Identity=27.6699029126214, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24586587, Length=95, Percent_Identity=35.7894736842105, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45549022, Length=207, Percent_Identity=24.6376811594203, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221330053, Length=200, Percent_Identity=23.5, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583747, Length=203, Percent_Identity=26.1083743842365, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583749, Length=203, Percent_Identity=26.1083743842365, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583745, Length=217, Percent_Identity=26.7281105990783, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586599, Length=196, Percent_Identity=25.5102040816327, Blast_Score=70, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24586589, Length=94, Percent_Identity=35.1063829787234, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.54 [H]

Molecular weight: Translated: 77075; Mature: 77075

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRSLFLKSHLTPHPIKAHSMIRHSLLFALVLTASSPLYALAQASHSPVSSTASLHLEP
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEH
LSWWAGMHNPKLQLMLHSDALPTDAAQLKVEITGQDVLFEGIEQTDNPHYLFINLDLRQA
HHHHCCCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEEEEEHHCC
KPQTFDIRILAGDKQLLQLPYTLATRAEGSRERQGFNNKDVIYLITPDRFANGNPSNDNQ
CCCEEEEEEEECCHHHHHCCHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCH
SDMLEHAAPDNSDGRHGGDIEGIRQHLDYLAKLGVTQLWINPLLENNQAQYSYHGYAMTN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCEEEEE
LYRVDPRFGSNQDYQRLVTEAKQHGLGVIKDVVLNHIGSNHWWLKDLPSSDWLNVPQQPN
EEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCC
ATDTQTAPAASSTSLSSTNLGFTSHRRTTVQDPYADMQDKRDFVDGWFVDSMPDLNQRNP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEECCCCCCCCCCCC
KLATYLIQNSIWWVEYAGLSGIREDTYSYADKAFLTQWTKALMAEYPHFNIVGEEWTANP
CCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
ITVSYWQKGKINADGYSSDLPSLMDFPLYEKLLESLNEPEGWDTGFIKLYEMLANDVVYA
EEEEEECCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEE
TPSQLVLFEGNHDTNRIYSLLKENLPLYQMALTYVLTAKRIPQLFYGTEVLMTSPTEGRH
CCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
DGVVRSDFPGGFANNKVNGFSGKGLNAKQQQALAFVRTLLNYRKHSTALQTGDLLHFVPQ
CCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
NGIYVQFRCVAKQAAAGNACYQADTDKVMVIYNKNDQAQTLDLSRFSKVLAGNTSANSVL
CCEEEEEEEEEHHHCCCCCEEECCCCCEEEEECCCCCCEEECHHHHHHHHCCCCCCCCCC
SGDKLKLNQSLVLPHAGVMLLELNHQ
CCCCEEECCEEECCCCCEEEEEECCC
>Mature Secondary Structure
MIRRSLFLKSHLTPHPIKAHSMIRHSLLFALVLTASSPLYALAQASHSPVSSTASLHLEP
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEH
LSWWAGMHNPKLQLMLHSDALPTDAAQLKVEITGQDVLFEGIEQTDNPHYLFINLDLRQA
HHHHCCCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEEEEEHHCC
KPQTFDIRILAGDKQLLQLPYTLATRAEGSRERQGFNNKDVIYLITPDRFANGNPSNDNQ
CCCEEEEEEEECCHHHHHCCHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCH
SDMLEHAAPDNSDGRHGGDIEGIRQHLDYLAKLGVTQLWINPLLENNQAQYSYHGYAMTN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCEEEEE
LYRVDPRFGSNQDYQRLVTEAKQHGLGVIKDVVLNHIGSNHWWLKDLPSSDWLNVPQQPN
EEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCC
ATDTQTAPAASSTSLSSTNLGFTSHRRTTVQDPYADMQDKRDFVDGWFVDSMPDLNQRNP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEECCCCCCCCCCCC
KLATYLIQNSIWWVEYAGLSGIREDTYSYADKAFLTQWTKALMAEYPHFNIVGEEWTANP
CCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
ITVSYWQKGKINADGYSSDLPSLMDFPLYEKLLESLNEPEGWDTGFIKLYEMLANDVVYA
EEEEEECCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEE
TPSQLVLFEGNHDTNRIYSLLKENLPLYQMALTYVLTAKRIPQLFYGTEVLMTSPTEGRH
CCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
DGVVRSDFPGGFANNKVNGFSGKGLNAKQQQALAFVRTLLNYRKHSTALQTGDLLHFVPQ
CCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
NGIYVQFRCVAKQAAAGNACYQADTDKVMVIYNKNDQAQTLDLSRFSKVLAGNTSANSVL
CCEEEEEEEEEHHHCCCCCEEECCCCCEEEEECCCCCCEEECHHHHHHHHCCCCCCCCCC
SGDKLKLNQSLVLPHAGVMLLELNHQ
CCCCEEECCEEECCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644767; 8425614 [H]