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The map label for this gene is celR [H]

Identifier: 117920695

GI number: 117920695

Start: 2713809

End: 2714849

Strand: Reverse

Name: celR [H]

Synonym: Shewana3_2252

Alternate gene names: 117920695

Gene position: 2714849-2713809 (Counterclockwise)

Preceding gene: 117920696

Following gene: 117920677

Centisome position: 54.6

GC content: 48.51

Gene sequence:

>1041_bases
ATGGCATCGAAAGCGACCTCGATTGATATTGCTTATCGTGCTGGTGTATCCCAGTCTACGGTTTCCCGAGCCCTGAGAAA
TAGTCCGTTAGTCAATTTAGAAACCCGTCAACGGATCCAAGCGATTGCGAAAGAACTCAACTACAAAGTCGATAAAAACG
CCAGTAACCTACGCACCCAAAATAGCCACACCTTAGCCCTGTTGTTGTGTGAAGATCCCACCAATGATGATTCACTGATC
AACCCCTTTTTTCTGTCGATGTTAGGCTCAATCACGCGGGCGACGGCGCAGCAAGGTTATGATTTACTCGTGTCATTTCA
ACAGCTTTCCAGTGATTGGCATGCCGATTATGAAGATAGCAATAAAGCCGATGGCATTATTTTGCTAGGGTACGGCGACT
ACATGGATTACGAGCAAAAGCTCGAAAAGTTGCTGGCACAGAACACCCATTTTGTGATTTGGGGCGCAGAGCATAACAAT
AAATCCGTGTTATCCATCGGCTGCGATAATCACCAAGGCGGCCTAATTGCCACTGAGCATTTAATTTCCCTCGGTCGTAA
GGATTTTGCATTTCTGGGCGGGGCATCGAGTCATTGCCCTGAGTTCAGGGACAGATATTTAGGCCATGTGAAAGCACTGC
AAACTGCCGGGCTGACTGTCGATCCTCGTCGGCAGATTTCGGCTATTAGCACTGAGGGGGCGGGTTACTATGCTACTTTG
GAGTTGCTCGATAAAGGATTGAATTTCGACGCGATTTTTGCCGCCAGTGATTTAATCGCCATAGGTGCGATCCGCGCCTT
AAAAGAGAAGGGGATTTTAGTGCCCGAGCAAGTCGCAGTCGTCGGGTATGATGATATTCCCGTAGCAAGCTTTGCCAATC
CGCCGTTAACCACGATTAAGCAAAATACCCAGTTAGCGGGGGAGATTTTAGTGGAAAGTCTACTTAAGCTTATTCGTGGT
CAAGCGGTGAGCCCGCAGTTGATCCCAACGACGTTAGTGGTACGCAGTTCATGCGGGATTGACTGTACGCCGCTCGCTTG
A

Upstream 100 bases:

>100_bases
ATCCCTGCTAACTTGTTAGTCAGTTGTAAACCCTTCGGCTATACAAGTCATTCGAACTGTTTTAGTATCGCCTCAAAATA
ATAATTATGGTGACCTTGCT

Downstream 100 bases:

>100_bases
GTCGCGTCTATTTTGATTGAATCATTAAGCCGAGTGATTGATAACACTCGGCTTTTTTATTGATGCGTTATACTCATTTT
GCCTGATGGAGGACCAAAAT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQNSHTLALLLCEDPTNDDSLI
NPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDSNKADGIILLGYGDYMDYEQKLEKLLAQNTHFVIWGAEHNN
KSVLSIGCDNHQGGLIATEHLISLGRKDFAFLGGASSHCPEFRDRYLGHVKALQTAGLTVDPRRQISAISTEGAGYYATL
ELLDKGLNFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIKQNTQLAGEILVESLLKLIRG
QAVSPQLIPTTLVVRSSCGIDCTPLA

Sequences:

>Translated_346_residues
MASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQNSHTLALLLCEDPTNDDSLI
NPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDSNKADGIILLGYGDYMDYEQKLEKLLAQNTHFVIWGAEHNN
KSVLSIGCDNHQGGLIATEHLISLGRKDFAFLGGASSHCPEFRDRYLGHVKALQTAGLTVDPRRQISAISTEGAGYYATL
ELLDKGLNFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIKQNTQLAGEILVESLLKLIRG
QAVSPQLIPTTLVVRSSCGIDCTPLA
>Mature_345_residues
ASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQNSHTLALLLCEDPTNDDSLIN
PFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDSNKADGIILLGYGDYMDYEQKLEKLLAQNTHFVIWGAEHNNK
SVLSIGCDNHQGGLIATEHLISLGRKDFAFLGGASSHCPEFRDRYLGHVKALQTAGLTVDPRRQISAISTEGAGYYATLE
LLDKGLNFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIKQNTQLAGEILVESLLKLIRGQ
AVSPQLIPTTLVVRSSCGIDCTPLA

Specific function: Binds to the 14-bp operator sequence 5'-TGGGAGCGCTCCCA- 3' in the upstream regions of cellulase genes; these operator sequences are modulated by cellobiose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=28.7833827893175, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=29.3255131964809, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790194, Length=320, Percent_Identity=28.75, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=31.6715542521994, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=29.2358803986711, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789202, Length=302, Percent_Identity=28.1456953642384, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=26.9794721407625, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787580, Length=337, Percent_Identity=29.3768545994065, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789456, Length=338, Percent_Identity=26.0355029585799, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994940, Length=327, Percent_Identity=24.4648318042813, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790715, Length=322, Percent_Identity=27.0186335403727, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790689, Length=340, Percent_Identity=25.8823529411765, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787906, Length=349, Percent_Identity=24.3553008595989, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37605; Mature: 37474

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQ
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCEECCCCHHCCCC
NSHTLALLLCEDPTNDDSLINPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDS
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
NKADGIILLGYGDYMDYEQKLEKLLAQNTHFVIWGAEHNNKSVLSIGCDNHQGGLIATEH
CCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
LISLGRKDFAFLGGASSHCPEFRDRYLGHVKALQTAGLTVDPRRQISAISTEGAGYYATL
HHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCEEEH
ELLDKGLNFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIK
HHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHH
QNTQLAGEILVESLLKLIRGQAVSPQLIPTTLVVRSSCGIDCTPLA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQ
CCCCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCEECCCCHHCCCC
NSHTLALLLCEDPTNDDSLINPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDS
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
NKADGIILLGYGDYMDYEQKLEKLLAQNTHFVIWGAEHNNKSVLSIGCDNHQGGLIATEH
CCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
LISLGRKDFAFLGGASSHCPEFRDRYLGHVKALQTAGLTVDPRRQISAISTEGAGYYATL
HHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCEEEH
ELLDKGLNFDAIFAASDLIAIGAIRALKEKGILVPEQVAVVGYDDIPVASFANPPLTTIK
HHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHH
QNTQLAGEILVESLLKLIRGQAVSPQLIPTTLVVRSSCGIDCTPLA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10224066 [H]