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The map label for this gene is gloA [H]

Identifier: 117920675

GI number: 117920675

Start: 2684707

End: 2685117

Strand: Direct

Name: gloA [H]

Synonym: Shewana3_2232

Alternate gene names: 117920675

Gene position: 2684707-2685117 (Clockwise)

Preceding gene: 117920666

Following gene: 117920678

Centisome position: 53.99

GC content: 50.85

Gene sequence:

>411_bases
ATGTCACAACTGTTACACACCATGCTTCGCGTCGGTAACTTAGAACGCAGTATCGCTTTTTACACTCAAGTGCTGGGTAT
GAAGCTGCTCCGCACCTCAGAAAACCCAGAGTACAAATATTCCCTCGCCTTCGTGGGTTTCGGTGAAGAATCAACGGGCC
AGGCCGTCATCGAGTTGACCTACAACTGGGGAACGGAAAAATACGATCTCGGCACGGCCTTTGGTCATATTGCCATCGGT
GATGAAGACATTTATGCCCGCTGCGAAGCCATTGCCGCTGCGGGTGGTAAAGTGACTCGCGCACCCGGCCCTGTTGCTGG
TGGCACCACTGAAATCGCCTTTGTGGAAGACCCCGACGGTTATAAGATTGAATTTATTCAAATGAAATCGGCGACCCAAG
GTTTAGGTTAA

Upstream 100 bases:

>100_bases
AATCAAGACTAGGCAATCCATTTATTCGATTAACATTCCGGCATGACTTACGACAAATTTTGATATACTGCCGCCGTAAT
TCATTGACCGAGATAATCCT

Downstream 100 bases:

>100_bases
GCCTATTACCTTCCCGCCTGATATAAAACGGCGTTTGCATACCCTGCAAACGCCGTTTTCTTTGCTCATAAATAAATGCC
AATACTAGGGATTACCGCTT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSQLLHTMLRVGNLERSIAFYTQVLGMKLLRTSENPEYKYSLAFVGFGEESTGQAVIELTYNWGTEKYDLGTAFGHIAIG
DEDIYARCEAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIEFIQMKSATQGLG

Sequences:

>Translated_136_residues
MSQLLHTMLRVGNLERSIAFYTQVLGMKLLRTSENPEYKYSLAFVGFGEESTGQAVIELTYNWGTEKYDLGTAFGHIAIG
DEDIYARCEAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIEFIQMKSATQGLG
>Mature_135_residues
SQLLHTMLRVGNLERSIAFYTQVLGMKLLRTSENPEYKYSLAFVGFGEESTGQAVIELTYNWGTEKYDLGTAFGHIAIGD
EDIYARCEAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIEFIQMKSATQGLG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=147, Percent_Identity=35.3741496598639, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=71.6417910447761, Blast_Score=194, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6323639, Length=140, Percent_Identity=42.1428571428571, Blast_Score=105, Evalue=3e-24,
Organism=Drosophila melanogaster, GI20129743, Length=149, Percent_Identity=36.241610738255, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920404, Length=139, Percent_Identity=33.0935251798561, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14735; Mature: 14604

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLLHTMLRVGNLERSIAFYTQVLGMKLLRTSENPEYKYSLAFVGFGEESTGQAVIELT
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEEEEE
YNWGTEKYDLGTAFGHIAIGDEDIYARCEAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDG
ECCCCCEEECCCCEEEEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIEFIQMKSATQGLG
CEEEEEEECCCCCCCC
>Mature Secondary Structure 
SQLLHTMLRVGNLERSIAFYTQVLGMKLLRTSENPEYKYSLAFVGFGEESTGQAVIELT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEEEEE
YNWGTEKYDLGTAFGHIAIGDEDIYARCEAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDG
ECCCCCEEECCCCEEEEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIEFIQMKSATQGLG
CEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739; 8021208 [H]