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The map label for this gene is cmlA [H]

Identifier: 117920647

GI number: 117920647

Start: 2644839

End: 2646047

Strand: Reverse

Name: cmlA [H]

Synonym: Shewana3_2204

Alternate gene names: 117920647

Gene position: 2646047-2644839 (Counterclockwise)

Preceding gene: 117920648

Following gene: 117920646

Centisome position: 53.22

GC content: 49.21

Gene sequence:

>1209_bases
ATGACAAAATTTAATCAAAACCCGCAAGACCGCAAATGGGATTATACCTTTGCGTACTCACTCCTATTACTTATTCCCTT
CGATTTACTCGCTTCTCTTGGCATGGATCTGTATCTGCCTGCCATCAATGATATTCGCCAGAGTTTTAGTGTTAGCCAAG
GTCAAATGCAGCTGACTTTAACCCTCTATATGGCGATTTTAGGCTTAGGCCAGTTGCTATTTGGACCGCTATCCGACGCA
ATAGGGCGCAGGCGAGTGGTGATTGCCGGAGCCTTAGTTTACGCAGGTTCCAGTTTGGCGATGGCATTGACCGACAGCTT
CAGTGTGTTTCTGCTCCTTCGGATTGTCCAGGCACTGAGCGCCTCGGCCGTGCTAGTGGCGGCATTTGCGACAGTGCGAG
ATGTGTTTGCCGATAGGGAAGAAGGCGTCGTTATCTATGCCATTATGGGCTCAGTGCTCGCGGCCGTGCCTGTGGTAGCA
CCTTTTATTGGCTCGCTTATCGATGGGCTTTGGCATTGGCGAGGGATCTTCTACACCTTAGCGATATTTGCTGCCCTAGT
AGGCTTGCATGCCTTACTGAAATGGCCTGAAACCCGCCCAGCGCAGACCCATCCCTTTGAGTGGTCGAGATGTTTTAACA
TCATTCGCGCTAAGGCGTTTTTAGGCTATACCTTGGCCTACGCCACGGCCATGGGGGCCTTCTTTGTCTATTTTTCGGCA
TCACCGAGCATACTGATGGACAAATTAGGACTCGATAAGGTGACCTTTAGCCTGTGGTTTGGCTCGGTTGCGATAGTCAT
GATAATCACCACCCGCTTTGTTAAGCATCTTGTCAAACGTTGGGGCACACAGGGAACTGTGCTAAGAGGCTTGATTGGCA
TGATGCTAAGTAGCGGCTTATTGTATGGGCTGGAATGGCTAAATGGGCTGAGTTTACTTGGCTTTATGCTGCCTATGTAT
TTCATCGGAGTGCATATCGCCTTAACCTGCGCCGTAACCGCTAATGGTGCCTTAGCGGCGTTTTCCCATTCTGCGGGGCT
GGCAACCGCTCTGTATTATGCCATTGAGAGTCTGTTCTTAAGTGCCTTAGTCAGTCTGTTGATTTGGCTGTTGCCCTCGG
GTACTACGCTGGTGATCTGTAGTTATATTTTGATCTCGGCGTCACTATCACTGCTATTTATTCTCTCCATTAGAGCAGAT
AACAAATAG

Upstream 100 bases:

>100_bases
AGTTATTCGATAGGTTAAAAACGGAGGTTAAAGATGAAGAGGGCGGTGCCTTTTATCCAATTATCGCTGTAAACATTCAT
AGCAATAATTGGATTTTAAC

Downstream 100 bases:

>100_bases
CCTCAGCTCCATTATCGTCCATTAAAAGCCGAGAATAGCTCGGCTTTTTTTGCATGCTTTATTGGTTGTTATTTGCTCGA
AATTACCGTACGTATCACTA

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTKFNQNPQDRKWDYTFAYSLLLLIPFDLLASLGMDLYLPAINDIRQSFSVSQGQMQLTLTLYMAILGLGQLLFGPLSDA
IGRRRVVIAGALVYAGSSLAMALTDSFSVFLLLRIVQALSASAVLVAAFATVRDVFADREEGVVIYAIMGSVLAAVPVVA
PFIGSLIDGLWHWRGIFYTLAIFAALVGLHALLKWPETRPAQTHPFEWSRCFNIIRAKAFLGYTLAYATAMGAFFVYFSA
SPSILMDKLGLDKVTFSLWFGSVAIVMIITTRFVKHLVKRWGTQGTVLRGLIGMMLSSGLLYGLEWLNGLSLLGFMLPMY
FIGVHIALTCAVTANGALAAFSHSAGLATALYYAIESLFLSALVSLLIWLLPSGTTLVICSYILISASLSLLFILSIRAD
NK

Sequences:

>Translated_402_residues
MTKFNQNPQDRKWDYTFAYSLLLLIPFDLLASLGMDLYLPAINDIRQSFSVSQGQMQLTLTLYMAILGLGQLLFGPLSDA
IGRRRVVIAGALVYAGSSLAMALTDSFSVFLLLRIVQALSASAVLVAAFATVRDVFADREEGVVIYAIMGSVLAAVPVVA
PFIGSLIDGLWHWRGIFYTLAIFAALVGLHALLKWPETRPAQTHPFEWSRCFNIIRAKAFLGYTLAYATAMGAFFVYFSA
SPSILMDKLGLDKVTFSLWFGSVAIVMIITTRFVKHLVKRWGTQGTVLRGLIGMMLSSGLLYGLEWLNGLSLLGFMLPMY
FIGVHIALTCAVTANGALAAFSHSAGLATALYYAIESLFLSALVSLLIWLLPSGTTLVICSYILISASLSLLFILSIRAD
NK
>Mature_401_residues
TKFNQNPQDRKWDYTFAYSLLLLIPFDLLASLGMDLYLPAINDIRQSFSVSQGQMQLTLTLYMAILGLGQLLFGPLSDAI
GRRRVVIAGALVYAGSSLAMALTDSFSVFLLLRIVQALSASAVLVAAFATVRDVFADREEGVVIYAIMGSVLAAVPVVAP
FIGSLIDGLWHWRGIFYTLAIFAALVGLHALLKWPETRPAQTHPFEWSRCFNIIRAKAFLGYTLAYATAMGAFFVYFSAS
PSILMDKLGLDKVTFSLWFGSVAIVMIITTRFVKHLVKRWGTQGTVLRGLIGMMLSSGLLYGLEWLNGLSLLGFMLPMYF
IGVHIALTCAVTANGALAAFSHSAGLATALYYAIESLFLSALVSLLIWLLPSGTTLVICSYILISASLSLLFILSIRADN
K

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI48994889, Length=362, Percent_Identity=27.3480662983425, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788509, Length=334, Percent_Identity=28.7425149700599, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1790794, Length=267, Percent_Identity=32.2097378277154, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1790146, Length=257, Percent_Identity=27.2373540856031, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082312, Length=340, Percent_Identity=25, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787065, Length=245, Percent_Identity=26.1224489795918, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=169, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322070, Length=198, Percent_Identity=25.7575757575758, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322071, Length=206, Percent_Identity=24.2718446601942, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319657, Length=168, Percent_Identity=29.7619047619048, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43864; Mature: 43732

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure 
TKFNQNPQDRKWDYTFAYSLLLLIPFDLLASLGMDLYLPAINDIRQSFSVSQGQMQLTL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCHHHHHH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HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]