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The map label for this gene is pykA [H]

Identifier: 117920597

GI number: 117920597

Start: 2579323

End: 2580762

Strand: Direct

Name: pykA [H]

Synonym: Shewana3_2153

Alternate gene names: 117920597

Gene position: 2579323-2580762 (Clockwise)

Preceding gene: 117920596

Following gene: 117920600

Centisome position: 51.87

GC content: 49.24

Gene sequence:

>1440_bases
ATGTTCCGCAGAACCAAAATCGTCACCACTCTGGGCCCCGCCACTGACCGTGACGATAACTTACGCCGTATTATCGCGGC
AGGTGCAAACGTTGTTCGTCTTAACTTCTCCCACGGCTCCCCCGAAGATCATCTCAAACGCGCGACTCAAGCTCGCGAAA
TTGCAAAAGAATTAGGTGTCCATGTTGCTATTTTAGGTGACTTACAAGGTCCTAAGATCCGTGTATCTACCTTTAAAGAC
AACAAGAAAGTTCAACTGAACTTAGGTCAAGCCTACATTCTGGATGCCGAGTTAGGTAAAGGCGAAGGCGACGAGAATCA
AGTCGGTATCGACTATAAGCAATTGCCAGACGATGTGAATGTCGGCGATATCCTGATGCTCGATGACGGCCGTGTTCAAC
TGCGTGTTGAGCGCGTTGAGGGTCGTAAAGTGCACACGACTGTTACCGTTGCTGGCCCTCTATCAAACAATAAAGGGATC
AACAAACAAGGTGGTGGTCTATCGGCAGCGGCTCTGACTGAAAAAGACAAGGCAGACATTCTGACTGCGGCCATGATCCA
AGTGGATTACTTAGCCGTGTCTTTCCCACGTAGCGGCGCCGATCTGGAATACGCCCGTTCTTTAGCACAACAAGCTGGCA
GCAATGCACTGATCGTCGCGAAAGTAGAACGTGCTGAAGCCGTTGCTAGCGAAGAAGCGATGGATGATGTGATTTTAGCC
TCTGACGTAGTCATGGTTGCCCGTGGTGACTTAGGGGTTGAGATTGGTGACGCAGCACTGGTTGCAGTGCAGAAGAAGCT
TATCGCTCGCTCTCGTCAACTGAATAAAATCGTGATCACTGCGACTCAAATGATGGAGTCAATGATTTCGAGCCCAATGC
CAACTCGCGCGGAAGTCATGGACGTGGCCAACGCCGTGCTCGATGGTACAGACGCTGTGATGCTGTCTGCCGAAACCGCT
GCTGGTGACTTCCCAGAAGAAACCGTTAAGGCGATGGCGAATGTGTGTATCGGTGCCGAATCTCACCCAAGCGTGAAAGT
GTCTAAGCACAGATTAGATGCACGTTTTACTTCAGTTGAAGAAACGATCGCACTCTCTACTATGTATGCCGCTAACCACT
TAGAAGGCGTTAAAGCGATTATTGCCTTAACTGAGTCTGGCGCAACACCTAAACTGATGTCACGTATCAGTTCATCTTTA
CCAATTCTTGGTTTATCACGCCATGAGACAACGCTGGCGAAGATGGCACTGTACCGTGGTGTGTTACCGATTTACTTCGA
CTCAACCATTTATCCTGCCGATGAGTTAGCGCAAAAAGCGTTGGAATCATTAACAGCTGCGGGTTATCTGCAGAGTGGCG
ACTTGGTATTAATGACCAAGGGCGATGCGATGGAAACTATCGGCGGCACTAACACTTGTAAAGTGTTGATCGTTGCATAA

Upstream 100 bases:

>100_bases
AAACATAACTTTAGATCAATTTGATTGTAACTTTACTACATAATTTGGAATTGCATGGTAATATAGCCCCAAGATTTTTT
TAACTTTAACGGAGTATCTT

Downstream 100 bases:

>100_bases
TTTTTGCTGATTGGCGTTAGCTAGACGTAAAGAGTAAGGCTAACCGTCATCAAGCTTAAAAACAAAAACCACCTCAATTG
AGGTGGTTTTTTTATATCTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGVHVAILGDLQGPKIRVSTFKD
NKKVQLNLGQAYILDAELGKGEGDENQVGIDYKQLPDDVNVGDILMLDDGRVQLRVERVEGRKVHTTVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKADILTAAMIQVDYLAVSFPRSGADLEYARSLAQQAGSNALIVAKVERAEAVASEEAMDDVILA
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMANVCIGAESHPSVKVSKHRLDARFTSVEETIALSTMYAANHLEGVKAIIALTESGATPKLMSRISSSL
PILGLSRHETTLAKMALYRGVLPIYFDSTIYPADELAQKALESLTAAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA

Sequences:

>Translated_479_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGVHVAILGDLQGPKIRVSTFKD
NKKVQLNLGQAYILDAELGKGEGDENQVGIDYKQLPDDVNVGDILMLDDGRVQLRVERVEGRKVHTTVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKADILTAAMIQVDYLAVSFPRSGADLEYARSLAQQAGSNALIVAKVERAEAVASEEAMDDVILA
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMANVCIGAESHPSVKVSKHRLDARFTSVEETIALSTMYAANHLEGVKAIIALTESGATPKLMSRISSSL
PILGLSRHETTLAKMALYRGVLPIYFDSTIYPADELAQKALESLTAAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA
>Mature_479_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGVHVAILGDLQGPKIRVSTFKD
NKKVQLNLGQAYILDAELGKGEGDENQVGIDYKQLPDDVNVGDILMLDDGRVQLRVERVEGRKVHTTVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKADILTAAMIQVDYLAVSFPRSGADLEYARSLAQQAGSNALIVAKVERAEAVASEEAMDDVILA
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMANVCIGAESHPSVKVSKHRLDARFTSVEETIALSTMYAANHLEGVKAIIALTESGATPKLMSRISSSL
PILGLSRHETTLAKMALYRGVLPIYFDSTIYPADELAQKALESLTAAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=34.8884381338742, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=34.8884381338742, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=34.8884381338742, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=34.0080971659919, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=34.0080971659919, Blast_Score=239, Evalue=5e-63,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=34.2560553633218, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=34.2560553633218, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=32.3404255319149, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=32.3404255319149, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=31.7757009345794, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=71.9077568134172, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=482, Percent_Identity=36.0995850622407, Blast_Score=278, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=36.7556468172485, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=493, Percent_Identity=33.0628803245436, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71984406, Length=493, Percent_Identity=33.0628803245436, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17506829, Length=493, Percent_Identity=33.0628803245436, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71984413, Length=493, Percent_Identity=33.0628803245436, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319279, Length=477, Percent_Identity=35.4297693920335, Blast_Score=249, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=32.0328542094456, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI28571814, Length=504, Percent_Identity=33.531746031746, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24648964, Length=504, Percent_Identity=33.531746031746, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24648966, Length=453, Percent_Identity=34.4370860927152, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24581235, Length=504, Percent_Identity=30.952380952381, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24646914, Length=278, Percent_Identity=35.2517985611511, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51090; Mature: 51090

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGV
CCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
HVAILGDLQGPKIRVSTFKDNKKVQLNLGQAYILDAELGKGEGDENQVGIDYKQLPDDVN
EEEEEECCCCCEEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCC
VGDILMLDDGRVQLRVERVEGRKVHTTVTVAGPLSNNKGINKQGGGLSAAALTEKDKADI
CCEEEEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHH
LTAAMIQVDYLAVSFPRSGADLEYARSLAQQAGSNALIVAKVERAEAVASEEAMDDVILA
HHHHHHEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHH
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVKAMANVCIGAESHPSVKVSKHRLDARFTSVE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHH
ETIALSTMYAANHLEGVKAIIALTESGATPKLMSRISSSLPILGLSRHETTLAKMALYRG
HHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHC
VLPIYFDSTIYPADELAQKALESLTAAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA
CCEEEECCCCCCHHHHHHHHHHHHHHHCEEECCCEEEEECCCCHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGSPEDHLKRATQAREIAKELGV
CCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
HVAILGDLQGPKIRVSTFKDNKKVQLNLGQAYILDAELGKGEGDENQVGIDYKQLPDDVN
EEEEEECCCCCEEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCC
VGDILMLDDGRVQLRVERVEGRKVHTTVTVAGPLSNNKGINKQGGGLSAAALTEKDKADI
CCEEEEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHH
LTAAMIQVDYLAVSFPRSGADLEYARSLAQQAGSNALIVAKVERAEAVASEEAMDDVILA
HHHHHHEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHH
SDVVMVARGDLGVEIGDAALVAVQKKLIARSRQLNKIVITATQMMESMISSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVKAMANVCIGAESHPSVKVSKHRLDARFTSVE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHH
ETIALSTMYAANHLEGVKAIIALTESGATPKLMSRISSSLPILGLSRHETTLAKMALYRG
HHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHC
VLPIYFDSTIYPADELAQKALESLTAAGYLQSGDLVLMTKGDAMETIGGTNTCKVLIVA
CCEEEECCCCCCHHHHHHHHHHHHHHHCEEECCCEEEEECCCCHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]