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The map label for this gene is pdhC [H]

Identifier: 117920573

GI number: 117920573

Start: 2553896

End: 2555491

Strand: Reverse

Name: pdhC [H]

Synonym: Shewana3_2129

Alternate gene names: 117920573

Gene position: 2555491-2553896 (Counterclockwise)

Preceding gene: 117920574

Following gene: 117920572

Centisome position: 51.4

GC content: 52.38

Gene sequence:

>1596_bases
ATGATTAAAGATTTTATTTTGCCGGACATTGGTGAAGGCGTTGTCGAGTGCGAACTCGTCGAGTGGTTGGTGCAAGAGGG
CGACACGATTGCTGAAGATCAGCCCATCGCCGATGTGATGACAGACAAAGCCTTAGTGCAAATTCCAGCGCCTTTTGCGG
GAGTCGTGACCAAGCTGTATTACACCAAAGGCGATATCGCTAAGGTGCATGCGCCGCTTTATGCTGTGCAGATTGAGTCT
GACGAAGCCGCGCCTGTAGCTGCGTCTCAGCCTGAAGCACACACAGCGACGCAAACACCTGCAAGTCAATCCAGCGCGGC
GGGTACGAGCGTAGAAGAGTTTCTGCTACCCGATATTGGCGAAGGCATTGTTGAGTGTGAACTGGTCGAATGGCTAGTCC
AAGAAGGCGATACTGTGGTTGAAGATCAACCCATCGCCGATGTGATGACAGATAAAGCCCTAGTGCAAATCCCTGCTATC
AAAGCGGGCAAAATTGTCAAATTGCACTACCGTAAGGGCCAGTTAGCGAAAGTACATGCACCACTATTTGCGATTGAGGT
CGAAGGTGGCGTTAGTGCGCCAGTCAGCCATGCACAGGAAGCCAGCGCCACCGCTGTGAATACCGCAGCACCAGTCGCTT
GCGCGGCGGTGTCCTCGGAGCCTGCTCGCCAAGGTAAAGCCTTAGCCAGCCCAGCCGTTCGCCGTATGGCACGTGCCTTA
GATATCGATTTAAGTCGCGTCCCTGGTAGTGGTAAACATGGCCGTGTGTATAAGGAAGACATTACTCGCTTCCAAGCACA
GGGCGGCGCTGTGCCTGCCGCTGCGCCAGTGGCAGCTGCCCCAACTGTGGCTCAATCGACCGTTTCTACCGCAGTGGCAA
GCGCTGCACGGGGTGACATTGTTGAGCCTATCCGTGGCGTGAAAGCCGTGATGGCGAAAATGATGGTGGAGTCGGTATCC
ACCATTCCACACTTCACCTATTGCGAAGAGTTTGATTTGACAGACTTAGTCGCTCTGCGTGAAAGCATGAAGGCCAAGTA
TTCCTCCGACGAAGTAAAGCTGACCATGATGCCATTCTTTATGAAGGCCATGTCGTTAGCCTTAACGCAGTTCCCTGTGC
TTAACAGTCAAGTGAATGCCGATTGTACGGAAATCACTTACAAAGCGCGTCATAACATAGGTATGGCGGTCGACTCTAAG
GTGGGATTATTGGTACCAAACGTGAAGGATGTGCAAGATAAGAGCATCCTCGAGGTTGCTGCCGAAATCACCCGCTTAAC
CAATGCTGCACGCAGCGGCCGTGTGGCGCCGGCGGATCTTAAAGAAGGCACCATCTCGATTTCGAATATTGGCGCCTTAG
GTGGCACAGTTGCAACGCCTATTATCAATAAGCCGGAAGTCGCGATTGTCGCATTAGGTAAGTTGCAAACCTTGCCGCGC
TTTAATGCGAAAGGTGAAGTCGAAGCACGTCAGATCATGCAAGTGAGTTGGTCTGGGGATCACCGCGTTATTGATGGCGG
CACCATTGCTCGTTTCTGTAACCTTTGGAAACAATACCTTGAGCAGCCGCAGGATATGCTATTAGCCATGCGCTAA

Upstream 100 bases:

>100_bases
AGATACCCCATATCCACTGGTCCACGAAAAAGAATATATGCCCGATGCGCTCAAAACCTTTGAAGCCATTAAGGCATCGG
TGAACTTCTAGGAGTCCGGT

Downstream 100 bases:

>100_bases
TCGGCTGTCAGCCAATACTTACGGCCAATCCAGCAAAAAAGGACGCAATTTAGCGTCCTTTTTTATTCGCAGGCCATAAA
TGGGTTAATCTTTTGATCCC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MIKDFILPDIGEGVVECELVEWLVQEGDTIAEDQPIADVMTDKALVQIPAPFAGVVTKLYYTKGDIAKVHAPLYAVQIES
DEAAPVAASQPEAHTATQTPASQSSAAGTSVEEFLLPDIGEGIVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAI
KAGKIVKLHYRKGQLAKVHAPLFAIEVEGGVSAPVSHAQEASATAVNTAAPVACAAVSSEPARQGKALASPAVRRMARAL
DIDLSRVPGSGKHGRVYKEDITRFQAQGGAVPAAAPVAAAPTVAQSTVSTAVASAARGDIVEPIRGVKAVMAKMMVESVS
TIPHFTYCEEFDLTDLVALRESMKAKYSSDEVKLTMMPFFMKAMSLALTQFPVLNSQVNADCTEITYKARHNIGMAVDSK
VGLLVPNVKDVQDKSILEVAAEITRLTNAARSGRVAPADLKEGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPR
FNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQDMLLAMR

Sequences:

>Translated_531_residues
MIKDFILPDIGEGVVECELVEWLVQEGDTIAEDQPIADVMTDKALVQIPAPFAGVVTKLYYTKGDIAKVHAPLYAVQIES
DEAAPVAASQPEAHTATQTPASQSSAAGTSVEEFLLPDIGEGIVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAI
KAGKIVKLHYRKGQLAKVHAPLFAIEVEGGVSAPVSHAQEASATAVNTAAPVACAAVSSEPARQGKALASPAVRRMARAL
DIDLSRVPGSGKHGRVYKEDITRFQAQGGAVPAAAPVAAAPTVAQSTVSTAVASAARGDIVEPIRGVKAVMAKMMVESVS
TIPHFTYCEEFDLTDLVALRESMKAKYSSDEVKLTMMPFFMKAMSLALTQFPVLNSQVNADCTEITYKARHNIGMAVDSK
VGLLVPNVKDVQDKSILEVAAEITRLTNAARSGRVAPADLKEGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPR
FNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQDMLLAMR
>Mature_531_residues
MIKDFILPDIGEGVVECELVEWLVQEGDTIAEDQPIADVMTDKALVQIPAPFAGVVTKLYYTKGDIAKVHAPLYAVQIES
DEAAPVAASQPEAHTATQTPASQSSAAGTSVEEFLLPDIGEGIVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAI
KAGKIVKLHYRKGQLAKVHAPLFAIEVEGGVSAPVSHAQEASATAVNTAAPVACAAVSSEPARQGKALASPAVRRMARAL
DIDLSRVPGSGKHGRVYKEDITRFQAQGGAVPAAAPVAAAPTVAQSTVSTAVASAARGDIVEPIRGVKAVMAKMMVESVS
TIPHFTYCEEFDLTDLVALRESMKAKYSSDEVKLTMMPFFMKAMSLALTQFPVLNSQVNADCTEITYKARHNIGMAVDSK
VGLLVPNVKDVQDKSILEVAAEITRLTNAARSGRVAPADLKEGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPR
FNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQDMLLAMR

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=46.1538461538462, Blast_Score=356, Evalue=4e-98,
Organism=Homo sapiens, GI31711992, Length=576, Percent_Identity=25.5208333333333, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI203098816, Length=465, Percent_Identity=27.3118279569892, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI203098753, Length=446, Percent_Identity=26.457399103139, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=30.3149606299213, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI260898739, Length=136, Percent_Identity=33.8235294117647, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1786305, Length=535, Percent_Identity=32.3364485981308, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1786946, Length=417, Percent_Identity=26.6187050359712, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=41.0672853828306, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI25146366, Length=418, Percent_Identity=29.4258373205742, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=27.6785714285714, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=23.4177215189873, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=426, Percent_Identity=26.5258215962441, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=27.3942093541203, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=40.8450704225352, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24582497, Length=308, Percent_Identity=29.8701298701299, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI20129315, Length=308, Percent_Identity=29.8701298701299, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=30.3738317757009, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56509; Mature: 56509

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDFILPDIGEGVVECELVEWLVQEGDTIAEDQPIADVMTDKALVQIPAPFAGVVTKLY
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEECCCHHHHHHHHH
YTKGDIAKVHAPLYAVQIESDEAAPVAASQPEAHTATQTPASQSSAAGTSVEEFLLPDIG
HCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
EGIVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAIKAGKIVKLHYRKGQLAKVHA
CHHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEECCCCCCCEEEEEECCCCEEEEEC
PLFAIEVEGGVSAPVSHAQEASATAVNTAAPVACAAVSSEPARQGKALASPAVRRMARAL
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
DIDLSRVPGSGKHGRVYKEDITRFQAQGGAVPAAAPVAAAPTVAQSTVSTAVASAARGDI
CCCHHCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCH
VEPIRGVKAVMAKMMVESVSTIPHFTYCEEFDLTDLVALRESMKAKYSSDEVKLTMMPFF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHH
MKAMSLALTQFPVLNSQVNADCTEITYKARHNIGMAVDSKVGLLVPNVKDVQDKSILEVA
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCEECCCCCCCCHHHHHHHH
AEITRLTNAARSGRVAPADLKEGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPR
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCEEEEEECHHHCCCC
FNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQDMLLAMR
CCCCCCCCHHHHEEEEECCCEEEECCHHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MIKDFILPDIGEGVVECELVEWLVQEGDTIAEDQPIADVMTDKALVQIPAPFAGVVTKLY
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEECCCHHHHHHHHH
YTKGDIAKVHAPLYAVQIESDEAAPVAASQPEAHTATQTPASQSSAAGTSVEEFLLPDIG
HCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
EGIVECELVEWLVQEGDTVVEDQPIADVMTDKALVQIPAIKAGKIVKLHYRKGQLAKVHA
CHHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEECCCCCCCEEEEEECCCCEEEEEC
PLFAIEVEGGVSAPVSHAQEASATAVNTAAPVACAAVSSEPARQGKALASPAVRRMARAL
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
DIDLSRVPGSGKHGRVYKEDITRFQAQGGAVPAAAPVAAAPTVAQSTVSTAVASAARGDI
CCCHHCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCH
VEPIRGVKAVMAKMMVESVSTIPHFTYCEEFDLTDLVALRESMKAKYSSDEVKLTMMPFF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHH
MKAMSLALTQFPVLNSQVNADCTEITYKARHNIGMAVDSKVGLLVPNVKDVQDKSILEVA
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCEECCCCCCCCHHHHHHHH
AEITRLTNAARSGRVAPADLKEGTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPR
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCEEEEEECHHHCCCC
FNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQDMLLAMR
CCCCCCCCHHHHEEEEECCCEEEECCHHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]