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The map label for this gene is dacB [H]

Identifier: 117920430

GI number: 117920430

Start: 2363811

End: 2365352

Strand: Reverse

Name: dacB [H]

Synonym: Shewana3_1985

Alternate gene names: 117920430

Gene position: 2365352-2363811 (Counterclockwise)

Preceding gene: 117920431

Following gene: 117920419

Centisome position: 47.57

GC content: 49.35

Gene sequence:

>1542_bases
ATGACGTACTCGCTAAACAAGGATAAGTTACAGGCATCATTCACTCGCCCCAAGATTAAAGGGTTACTCTTAGGTAGCTT
GGTAAGCATGCTACTGTCAACCCAAGCCCATGCGGCAATTCATCCGATGGATGAGTCGATATTGGCCAAGCAAATCGCCG
ATATTGCTCCGCGTCATTCGCAAGTGGCTTTGTTGGCCCGTGATTTAAGTACCAATACCCTGTTATACAGCCAGCAAGCC
GATACCTTATTTATCCCCGCCAGCACACAAAAAGTGCTGACCGCCGTTACCGCTTTGGCCACCCTTGGCCCAGATTTTCG
CTATGTCACTGAGCTGTGGAGTGATGCGCCTATTCGCCAAGGTCATATTGCGGGCAGTGTTTATCTGCGTTTTAGCGGCG
ATCCGACGCTAACTCAGGACGACCTTAAGGCCTTGTTTGCTCATCTACAAAAGCAAGGCATAACAAGCATTGAGGGGCAT
CTCTATCTTATCGGGGATAAACAAGAGCAATTACAAGCGCCTGGCTGGGTGTGGGATGATCTCGGGATTTGCTTTGCAGC
GCCGGTGTCGAGTTATATCATCAACCAAAACTGCGTGTACGGGCAGTTTGCGCCAAGCTCAGCCAAGCATGCTTCTGAGG
TGAAATTAAGGGCATCGAGTTTTGGGGTGAAAGTGAGTAGCGACGCCATATTCTCCCCCAAAGCAGGCCATGATTTTTGC
CAACTCGATTTAGTACGCCTTGGCCAGAATCAGTATCATCTACGGGGCTGCTATCCTGGCAATGAAGCGATACCGCTGGC
GATTGCCATTAGCGATCCTGAAAAATTTGCCATGGACACCCTAACAGCGACGCTCAAGGGTGAAATGTCACTCTCAGGTA
AAGTGAAGATAGGCAACGCCATCCCTTCGAAGGCAAAACTCATTGCCAGCCATAGCAGCGCACCCTTGCCTGAACTGCTC
AAAACCATGTTGCTGAAGTCGGATAACCTGATTGCCGATAGTTTGTTTAAGCGCGTGGGGCAAAGTTATTACAAAGCCCA
AGGCAGCTTTACCCATGGTGCAGCGGCTATGCGCCATATACTGACTGAGCTTGGGATTGATTTAACCAACGCCAATATTG
TCGATGGTTCAGGGCTTTCTCGCTATAACCTGTTGAGCGCAAAACAATTAGCCGATGTACTGGCACTCATTTACCAAGAT
GCACGCTTTCACAGCCTCATTGACAGTTTGCCCGAAGCTGGTGTCAGCGGCACACTGCAATATCGCTTGGGATACACTAA
ACCGCCCTTAAAACATCGAGTGTTTGCCAAGACTGGCTCGATGCAGGGTGTCGCCAATTTAGCTGGCTTTATGCGTTTAG
CGCAGCAGCGGGATATTCTCTTTGTCGTGCTCGAGAATGGCATCAGTCCTGAAACCAAAAAACAACGTAAGCCCGCCTTT
AGCGCTGACTTTTTAACTCGGCTACTGAGCGCTGTCGAAACTCAAACTCAGACCAGCCAAACAACGACCACTGAGCTCAA
CACCGTTAAGTTAAGTGATTAA

Upstream 100 bases:

>100_bases
TCGGATTGGTTTTATTTGTCACCCTAAGACTGTATCGTTTAATGGAAAAAGACGATGGCAGCAATGACCGCTAATGGTAA
AAATGATATTCAGGACACCT

Downstream 100 bases:

>100_bases
TATCCGATAAGGTGCAGAGTCATATACAAAGCAATTAAAAAGAAAAAGCACTGAATATCAGTGCTTTTTTATTGGGTTAA
TTCGAACGTTTAACGCGATT

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTYSLNKDKLQASFTRPKIKGLLLGSLVSMLLSTQAHAAIHPMDESILAKQIADIAPRHSQVALLARDLSTNTLLYSQQA
DTLFIPASTQKVLTAVTALATLGPDFRYVTELWSDAPIRQGHIAGSVYLRFSGDPTLTQDDLKALFAHLQKQGITSIEGH
LYLIGDKQEQLQAPGWVWDDLGICFAAPVSSYIINQNCVYGQFAPSSAKHASEVKLRASSFGVKVSSDAIFSPKAGHDFC
QLDLVRLGQNQYHLRGCYPGNEAIPLAIAISDPEKFAMDTLTATLKGEMSLSGKVKIGNAIPSKAKLIASHSSAPLPELL
KTMLLKSDNLIADSLFKRVGQSYYKAQGSFTHGAAAMRHILTELGIDLTNANIVDGSGLSRYNLLSAKQLADVLALIYQD
ARFHSLIDSLPEAGVSGTLQYRLGYTKPPLKHRVFAKTGSMQGVANLAGFMRLAQQRDILFVVLENGISPETKKQRKPAF
SADFLTRLLSAVETQTQTSQTTTTELNTVKLSD

Sequences:

>Translated_513_residues
MTYSLNKDKLQASFTRPKIKGLLLGSLVSMLLSTQAHAAIHPMDESILAKQIADIAPRHSQVALLARDLSTNTLLYSQQA
DTLFIPASTQKVLTAVTALATLGPDFRYVTELWSDAPIRQGHIAGSVYLRFSGDPTLTQDDLKALFAHLQKQGITSIEGH
LYLIGDKQEQLQAPGWVWDDLGICFAAPVSSYIINQNCVYGQFAPSSAKHASEVKLRASSFGVKVSSDAIFSPKAGHDFC
QLDLVRLGQNQYHLRGCYPGNEAIPLAIAISDPEKFAMDTLTATLKGEMSLSGKVKIGNAIPSKAKLIASHSSAPLPELL
KTMLLKSDNLIADSLFKRVGQSYYKAQGSFTHGAAAMRHILTELGIDLTNANIVDGSGLSRYNLLSAKQLADVLALIYQD
ARFHSLIDSLPEAGVSGTLQYRLGYTKPPLKHRVFAKTGSMQGVANLAGFMRLAQQRDILFVVLENGISPETKKQRKPAF
SADFLTRLLSAVETQTQTSQTTTTELNTVKLSD
>Mature_512_residues
TYSLNKDKLQASFTRPKIKGLLLGSLVSMLLSTQAHAAIHPMDESILAKQIADIAPRHSQVALLARDLSTNTLLYSQQAD
TLFIPASTQKVLTAVTALATLGPDFRYVTELWSDAPIRQGHIAGSVYLRFSGDPTLTQDDLKALFAHLQKQGITSIEGHL
YLIGDKQEQLQAPGWVWDDLGICFAAPVSSYIINQNCVYGQFAPSSAKHASEVKLRASSFGVKVSSDAIFSPKAGHDFCQ
LDLVRLGQNQYHLRGCYPGNEAIPLAIAISDPEKFAMDTLTATLKGEMSLSGKVKIGNAIPSKAKLIASHSSAPLPELLK
TMLLKSDNLIADSLFKRVGQSYYKAQGSFTHGAAAMRHILTELGIDLTNANIVDGSGLSRYNLLSAKQLADVLALIYQDA
RFHSLIDSLPEAGVSGTLQYRLGYTKPPLKHRVFAKTGSMQGVANLAGFMRLAQQRDILFVVLENGISPETKKQRKPAFS
ADFLTRLLSAVETQTQTSQTTTTELNTVKLSD

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=428, Percent_Identity=35.981308411215, Blast_Score=257, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 55748; Mature: 55616

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSLNKDKLQASFTRPKIKGLLLGSLVSMLLSTQAHAAIHPMDESILAKQIADIAPRHS
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHCCCCC
QVALLARDLSTNTLLYSQQADTLFIPASTQKVLTAVTALATLGPDFRYVTELWSDAPIRQ
CEEEEEECCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
GHIAGSVYLRFSGDPTLTQDDLKALFAHLQKQGITSIEGHLYLIGDKQEQLQAPGWVWDD
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCEEEEEECCHHHHCCCCCEECC
LGICFAAPVSSYIINQNCVYGQFAPSSAKHASEVKLRASSFGVKVSSDAIFSPKAGHDFC
CCHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHEEEEHHHCCEEECCCCEECCCCCCCHH
QLDLVRLGQNQYHLRGCYPGNEAIPLAIAISDPEKFAMDTLTATLKGEMSLSGKVKIGNA
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEECCEEEECCC
IPSKAKLIASHSSAPLPELLKTMLLKSDNLIADSLFKRVGQSYYKAQGSFTHGAAAMRHI
CCCHHHEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LTELGIDLTNANIVDGSGLSRYNLLSAKQLADVLALIYQDARFHSLIDSLPEAGVSGTLQ
HHHHCCEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEE
YRLGYTKPPLKHRVFAKTGSMQGVANLAGFMRLAQQRDILFVVLENGISPETKKQRKPAF
EEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCC
SADFLTRLLSAVETQTQTSQTTTTELNTVKLSD
CHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
>Mature Secondary Structure 
TYSLNKDKLQASFTRPKIKGLLLGSLVSMLLSTQAHAAIHPMDESILAKQIADIAPRHS
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHCCCCC
QVALLARDLSTNTLLYSQQADTLFIPASTQKVLTAVTALATLGPDFRYVTELWSDAPIRQ
CEEEEEECCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
GHIAGSVYLRFSGDPTLTQDDLKALFAHLQKQGITSIEGHLYLIGDKQEQLQAPGWVWDD
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCEEEEEECCHHHHCCCCCEECC
LGICFAAPVSSYIINQNCVYGQFAPSSAKHASEVKLRASSFGVKVSSDAIFSPKAGHDFC
CCHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHEEEEHHHCCEEECCCCEECCCCCCCHH
QLDLVRLGQNQYHLRGCYPGNEAIPLAIAISDPEKFAMDTLTATLKGEMSLSGKVKIGNA
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEECCEEEECCC
IPSKAKLIASHSSAPLPELLKTMLLKSDNLIADSLFKRVGQSYYKAQGSFTHGAAAMRHI
CCCHHHEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LTELGIDLTNANIVDGSGLSRYNLLSAKQLADVLALIYQDARFHSLIDSLPEAGVSGTLQ
HHHHCCEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEE
YRLGYTKPPLKHRVFAKTGSMQGVANLAGFMRLAQQRDILFVVLENGISPETKKQRKPAF
EEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCC
SADFLTRLLSAVETQTQTSQTTTTELNTVKLSD
CHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]