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The map label for this gene is thiH [H]

Identifier: 117920386

GI number: 117920386

Start: 2307669

End: 2308784

Strand: Direct

Name: thiH [H]

Synonym: Shewana3_1941

Alternate gene names: 117920386

Gene position: 2307669-2308784 (Clockwise)

Preceding gene: 117920385

Following gene: 117920387

Centisome position: 46.41

GC content: 50.18

Gene sequence:

>1116_bases
ATGAGTTTTGTCGACCAATTTGCCCGTATTGAACGGGATAAGTTATTGCTGGCGTTATATTCCTGCACGGCAGTGGAGGT
TGAGCGCGCCCTGATGCAACCCGAGGGCAATCTACAGAGTTTACTTGCCTTGTTGTCTCCAGCGGCCGAGCCCTATATCG
AAGAGATGGCGCAGCGCTCGGCGGCGCTCACTCGGCAACGCTTTGGGGCCAATATCGGACTCTATCTGCCCTTATACCTG
TCGAACCTGTGTGCCAATGAGTGCGACTATTGCGGCTTTAGCATGAGCAATAAGCTAAAGCGCAAAGTGCTCAATGAGCA
GGAAATTGCGGCTGAAATGGCGATTATCAAATTCCGTGGTTTTGACTCCATCTTGCTGGTGTCGGGAGAGCATGACACCA
AAGTGGGGATGGATTACTTTAAGCGCGTGTTACCCATTGTAAAACAGCAGTTTAGTTATATGGCCATGGAGGTTCAGCCG
CTTGAAGAGGTTGATTATCGTCAGCTTGTCGAGCTAGGGCTGGATGCTGTGATGGTGTATCAAGAAACCTATCAAGCGGC
GACCTATGCCAAGCATCATACCCGAGGCAATAAACAGGACTTTGCATATCGGCTCGCAACGCCCGACCGCGTTGCTAGTG
CGGGTGTCGATAAGATAGGCCTAGGTGTGCTACTAGGTTTAGATGACTGGCGGCTCGATGCTTTACTGATGGGCCATCAT
CTGGACTATTTAGAACGGAATTACTGGCGTACTCGTTTTAGTATTTCGTTACCACGTTTGCGGCCTTGTACCGGCGGTAT
AACACCAAAAGTGCATTTAACCGATCTTGGACTGGTGCAGATGATCTGTGCCTTCAGGCTTTTTAATCAGCAACTTGATA
TCAGTTTATCGACACGCGAGGCGCCATCGCTTCGGGACAATTTACTGCCGCTTGGGATAACACAGATGAGTGCGGGGAGC
TCGACACAGCCAGGCGGTTATCAGGCGCCAGAGAGCCAATTAGATCAGTTTGAGATAAGCGATGAACGTACTGTTGAGCA
GGTCATGACTCAGATGCGACTCAGGGGATTTAATCCGGTTTTCAAGGATTGGGAATCGGCTTGGATTGCGCGTTAA

Upstream 100 bases:

>100_bases
AGGACGCGGTGGATTGTGGGCGCCGCGCCTTTGAAGCGGGGCTTGGCCGAGTGCAAACCCAGGCTGTACATACCAGTCCA
CTAACGGGGTTCTTGTAGCA

Downstream 100 bases:

>100_bases
TAGGGGACTAAAAGTGATTTTAAAATGCCTTTATGGCGGGGGATAAACCTGTACTTTGCCTGAGGTTTGATTAATAATTA
ACCCAGTTATCACCAGATGC

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSFVDQFARIERDKLLLALYSCTAVEVERALMQPEGNLQSLLALLSPAAEPYIEEMAQRSAALTRQRFGANIGLYLPLYL
SNLCANECDYCGFSMSNKLKRKVLNEQEIAAEMAIIKFRGFDSILLVSGEHDTKVGMDYFKRVLPIVKQQFSYMAMEVQP
LEEVDYRQLVELGLDAVMVYQETYQAATYAKHHTRGNKQDFAYRLATPDRVASAGVDKIGLGVLLGLDDWRLDALLMGHH
LDYLERNYWRTRFSISLPRLRPCTGGITPKVHLTDLGLVQMICAFRLFNQQLDISLSTREAPSLRDNLLPLGITQMSAGS
STQPGGYQAPESQLDQFEISDERTVEQVMTQMRLRGFNPVFKDWESAWIAR

Sequences:

>Translated_371_residues
MSFVDQFARIERDKLLLALYSCTAVEVERALMQPEGNLQSLLALLSPAAEPYIEEMAQRSAALTRQRFGANIGLYLPLYL
SNLCANECDYCGFSMSNKLKRKVLNEQEIAAEMAIIKFRGFDSILLVSGEHDTKVGMDYFKRVLPIVKQQFSYMAMEVQP
LEEVDYRQLVELGLDAVMVYQETYQAATYAKHHTRGNKQDFAYRLATPDRVASAGVDKIGLGVLLGLDDWRLDALLMGHH
LDYLERNYWRTRFSISLPRLRPCTGGITPKVHLTDLGLVQMICAFRLFNQQLDISLSTREAPSLRDNLLPLGITQMSAGS
STQPGGYQAPESQLDQFEISDERTVEQVMTQMRLRGFNPVFKDWESAWIAR
>Mature_370_residues
SFVDQFARIERDKLLLALYSCTAVEVERALMQPEGNLQSLLALLSPAAEPYIEEMAQRSAALTRQRFGANIGLYLPLYLS
NLCANECDYCGFSMSNKLKRKVLNEQEIAAEMAIIKFRGFDSILLVSGEHDTKVGMDYFKRVLPIVKQQFSYMAMEVQPL
EEVDYRQLVELGLDAVMVYQETYQAATYAKHHTRGNKQDFAYRLATPDRVASAGVDKIGLGVLLGLDDWRLDALLMGHHL
DYLERNYWRTRFSISLPRLRPCTGGITPKVHLTDLGLVQMICAFRLFNQQLDISLSTREAPSLRDNLLPLGITQMSAGSS
TQPGGYQAPESQLDQFEISDERTVEQVMTQMRLRGFNPVFKDWESAWIAR

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=366, Percent_Identity=53.8251366120219, Blast_Score=425, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42115; Mature: 41984

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVDQFARIERDKLLLALYSCTAVEVERALMQPEGNLQSLLALLSPAAEPYIEEMAQRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHH
AALTRQRFGANIGLYLPLYLSNLCANECDYCGFSMSNKLKRKVLNEQEIAAEMAIIKFRG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHEECC
FDSILLVSGEHDTKVGMDYFKRVLPIVKQQFSYMAMEVQPLEEVDYRQLVELGLDAVMVY
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHCHHHHHHH
QETYQAATYAKHHTRGNKQDFAYRLATPDRVASAGVDKIGLGVLLGLDDWRLDALLMGHH
HHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCCHHCCEEEECCCCHHHHHHHHHHH
LDYLERNYWRTRFSISLPRLRPCTGGITPKVHLTDLGLVQMICAFRLFNQQLDISLSTRE
HHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCEEECCCC
APSLRDNLLPLGITQMSAGSSTQPGGYQAPESQLDQFEISDERTVEQVMTQMRLRGFNPV
CCCHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCHH
FKDWESAWIAR
HHHHHHHHCCC
>Mature Secondary Structure 
SFVDQFARIERDKLLLALYSCTAVEVERALMQPEGNLQSLLALLSPAAEPYIEEMAQRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHH
AALTRQRFGANIGLYLPLYLSNLCANECDYCGFSMSNKLKRKVLNEQEIAAEMAIIKFRG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHEECC
FDSILLVSGEHDTKVGMDYFKRVLPIVKQQFSYMAMEVQPLEEVDYRQLVELGLDAVMVY
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHCHHHHHHH
QETYQAATYAKHHTRGNKQDFAYRLATPDRVASAGVDKIGLGVLLGLDDWRLDALLMGHH
HHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCCHHCCEEEECCCCHHHHHHHHHHH
LDYLERNYWRTRFSISLPRLRPCTGGITPKVHLTDLGLVQMICAFRLFNQQLDISLSTRE
HHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCEEECCCC
APSLRDNLLPLGITQMSAGSSTQPGGYQAPESQLDQFEISDERTVEQVMTQMRLRGFNPV
CCCHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCHH
FKDWESAWIAR
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]