Click here to switch to the map view.

The map label for this gene is thiG [H]

Identifier: 117920385

GI number: 117920385

Start: 2306908

End: 2307666

Strand: Direct

Name: thiG [H]

Synonym: Shewana3_1940

Alternate gene names: 117920385

Gene position: 2306908-2307666 (Clockwise)

Preceding gene: 117920384

Following gene: 117920386

Centisome position: 46.4

GC content: 53.1

Gene sequence:

>759_bases
ATGTTAACGATTGCGGGTGTGGAATTTGAGTCACGATTATTTACGGGCACGGGTAAGTTCAGCTCAAGTCAGCTGATGCT
GGAGTCAATTAAGGCGTCTAAATCGCAATTGGTGACAGTTGCGATGAAAAGAATTGATTTAAAAACAGGGGCTGATGATT
TGCTATCGCCACTAAGGCAAGCGGGCGTGCGACTCTTGCCTAATACCTCGGGTGCGCGTAATGCTAAGGAAGCGATATTT
GCCGCCGAATTAGCGCGGGAAATGTTGGGAACACAATGGGTTAAACTTGAAATTCATCCCGATCCTAAATACCTAATGCC
CGATGCGGTCGAGACATTAGCCGCGGCCAAAACCCTGTGTGAACGCGGGTTTATTGTGATGCCCTACGTGCATGCCGATC
CTGTGCTGTGCCGACGCTTAGAAGAGGTTGGCTGCGCCGCCGTAATGCCATTGGCAAGCCCAATTGGGACGAATCAGGGG
CTGGTGACTGAGCCTTTTATCAAGATGATTATTGAGCAGGCAAAAGTGCCCGTGGTGATTGATGCGGGAATTGGCACGCC
CTCCCATGCGGCCCACGCGATGGAGCTGGGAGCCGATGCCGTGCTGGTTAATACCGCCATCGCGAGCAGTGCTTCTCCCA
TTGAAATGGCGCAGTGCTTTAAGGACGCGGTGGATTGTGGGCGCCGCGCCTTTGAAGCGGGGCTTGGCCGAGTGCAAACC
CAGGCTGTACATACCAGTCCACTAACGGGGTTCTTGTAG

Upstream 100 bases:

>100_bases
GTGCTTAACGAGGCCGTTGTACCCAAACATAGATGGTCATCTATCCTTTGTCATCATGAGGATAAGATAGATGTATTTGC
CGTTGTCGCCGGAGGTTAAG

Downstream 100 bases:

>100_bases
CAATGAGTTTTGTCGACCAATTTGCCCGTATTGAACGGGATAAGTTATTGCTGGCGTTATATTCCTGCACGGCAGTGGAG
GTTGAGCGCGCCCTGATGCA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLTIAGVEFESRLFTGTGKFSSSQLMLESIKASKSQLVTVAMKRIDLKTGADDLLSPLRQAGVRLLPNTSGARNAKEAIF
AAELAREMLGTQWVKLEIHPDPKYLMPDAVETLAAAKTLCERGFIVMPYVHADPVLCRRLEEVGCAAVMPLASPIGTNQG
LVTEPFIKMIIEQAKVPVVIDAGIGTPSHAAHAMELGADAVLVNTAIASSASPIEMAQCFKDAVDCGRRAFEAGLGRVQT
QAVHTSPLTGFL

Sequences:

>Translated_252_residues
MLTIAGVEFESRLFTGTGKFSSSQLMLESIKASKSQLVTVAMKRIDLKTGADDLLSPLRQAGVRLLPNTSGARNAKEAIF
AAELAREMLGTQWVKLEIHPDPKYLMPDAVETLAAAKTLCERGFIVMPYVHADPVLCRRLEEVGCAAVMPLASPIGTNQG
LVTEPFIKMIIEQAKVPVVIDAGIGTPSHAAHAMELGADAVLVNTAIASSASPIEMAQCFKDAVDCGRRAFEAGLGRVQT
QAVHTSPLTGFL
>Mature_252_residues
MLTIAGVEFESRLFTGTGKFSSSQLMLESIKASKSQLVTVAMKRIDLKTGADDLLSPLRQAGVRLLPNTSGARNAKEAIF
AAELAREMLGTQWVKLEIHPDPKYLMPDAVETLAAAKTLCERGFIVMPYVHADPVLCRRLEEVGCAAVMPLASPIGTNQG
LVTEPFIKMIIEQAKVPVVIDAGIGTPSHAAHAMELGADAVLVNTAIASSASPIEMAQCFKDAVDCGRRAFEAGLGRVQT
QAVHTSPLTGFL

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=65.0793650793651, Blast_Score=333, Evalue=6e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 26844; Mature: 26844

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIAGVEFESRLFTGTGKFSSSQLMLESIKASKSQLVTVAMKRIDLKTGADDLLSPLRQ
CEEEECCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGVRLLPNTSGARNAKEAIFAAELAREMLGTQWVKLEIHPDPKYLMPDAVETLAAAKTLC
CCCEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
ERGFIVMPYVHADPVLCRRLEEVGCAAVMPLASPIGTNQGLVTEPFIKMIIEQAKVPVVI
HCCCEEEEEECCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
DAGIGTPSHAAHAMELGADAVLVNTAIASSASPIEMAQCFKDAVDCGRRAFEAGLGRVQT
ECCCCCCHHHHHHHHHCCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QAVHTSPLTGFL
HHEECCCCCCCC
>Mature Secondary Structure
MLTIAGVEFESRLFTGTGKFSSSQLMLESIKASKSQLVTVAMKRIDLKTGADDLLSPLRQ
CEEEECCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGVRLLPNTSGARNAKEAIFAAELAREMLGTQWVKLEIHPDPKYLMPDAVETLAAAKTLC
CCCEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
ERGFIVMPYVHADPVLCRRLEEVGCAAVMPLASPIGTNQGLVTEPFIKMIIEQAKVPVVI
HCCCEEEEEECCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
DAGIGTPSHAAHAMELGADAVLVNTAIASSASPIEMAQCFKDAVDCGRRAFEAGLGRVQT
ECCCCCCHHHHHHHHHCCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QAVHTSPLTGFL
HHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]