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The map label for this gene is hydA [H]

Identifier: 117920325

GI number: 117920325

Start: 2227512

End: 2228648

Strand: Reverse

Name: hydA [H]

Synonym: Shewana3_1880

Alternate gene names: 117920325

Gene position: 2228648-2227512 (Counterclockwise)

Preceding gene: 117920336

Following gene: 117920324

Centisome position: 44.82

GC content: 53.74

Gene sequence:

>1137_bases
ATGGACACACATGCAGCCCTCTATGAACAGGGAAAGGCGCGCTTAGACGCACTGCGCCAACTCGCTCCCCGTCAACAGCA
AACATTAATCGAAAAATTACAACAACACGGCATCACTCGTCGTGACTTTATGAAGTGGAGCGCCATGGTGACGGGCATGC
TCGCCCTACCGCTGCCCTTTAGTAACTTAGTTGCCGAAGCCGCCGAACTGGCCGACCGTGTACCCTTAATCTGGTTACAC
ATGGCCGAATGTACTGGCTGCTCTGAATCCTTAGTGCGCGCCGATACGCCTAATTTAGATTCGCTGATCTTCGACCATAT
CTCGTTGGAATATCACGAAACCTTAATGGCCGCTGCCGGCTGGCAAGCAGAGGAAAACCTCGAGCACGCTCTTGAAACCT
ACAAGGGCCGTTATCTACTCGCCGTTGAAGGTGCCGTACCGACCGCTAATAACGGCAGCTTCTTAACCGTGGGCTGTAAA
GGTCATACCGGCTTAGAAATTATCAAGCATGCCGCCGAAGACGCCGCGGCGATTATTTCTGTGGGCACCTGCGCTTCCTT
TGGTGGCGTGCAAGCCGCCTACCCTAACCCCACAGGGGCGAAAGGGGTACACGAAGTCGTGAGCAAGCCTGTGATCAACT
TAGGTGGCTGCCCACCGAGTGAGAAAAACATCGTCGGCACCCTGATGTATTTCATCATGTTCGGCAAATTACCCGCGCTG
GATATGTTTAATCGTCCTAAATGGGCCTATGGCGCACGGGTGCACGATAACTGTGAACGCCGCGGCCGTTTCGATGCCGG
TGAGTTCGTTGAAGAGTTTGGCGATCACGGAGCGAAGGAAGGTTACTGCCTCTACAAAGTGGGCTGTAAAGGACCTTATA
CCTATAACAACTGTCCTACAGAGCGCTTTAACCACCATACCAGCTGGCCAGTGTTAGCGGGCCACGGTTGTATGGGCTGC
TCAGAACCTAACTTCTGGGATGATATGGCCGACTTTGAAAAACCCCTTGGCCGTCAACTACTCCATGGATTGGATGCTAC
CGCAGACACAGTCGGTGCGGTGATTTTAGGCGCGACCGTCGTCGGCATTGGAGCCCACGCCGTCGCCAGTATTTTTGCCA
AGCCGCTGGAGGAATAA

Upstream 100 bases:

>100_bases
GATATGCATCAAGAATTCTGTGTCGCCTAAGGCCTAAACTGGCAGTGTCAATTATGTAAACCCTTACCAATTTACATTCA
AATAACAAAAAGGTTGACCC

Downstream 100 bases:

>100_bases
CCCATGAGCAAGCGCGTTGTTATCGACCCTATCACCCGTATTGAGGGTCATTTACGTATCGAAGTCGAAGTTGATGAGAA
TAATGTCATCAACAAAGCAT

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MDTHAALYEQGKARLDALRQLAPRQQQTLIEKLQQHGITRRDFMKWSAMVTGMLALPLPFSNLVAEAAELADRVPLIWLH
MAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAGWQAEENLEHALETYKGRYLLAVEGAVPTANNGSFLTVGCK
GHTGLEIIKHAAEDAAAIISVGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCMGC
SEPNFWDDMADFEKPLGRQLLHGLDATADTVGAVILGATVVGIGAHAVASIFAKPLEE

Sequences:

>Translated_378_residues
MDTHAALYEQGKARLDALRQLAPRQQQTLIEKLQQHGITRRDFMKWSAMVTGMLALPLPFSNLVAEAAELADRVPLIWLH
MAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAGWQAEENLEHALETYKGRYLLAVEGAVPTANNGSFLTVGCK
GHTGLEIIKHAAEDAAAIISVGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCMGC
SEPNFWDDMADFEKPLGRQLLHGLDATADTVGAVILGATVVGIGAHAVASIFAKPLEE
>Mature_378_residues
MDTHAALYEQGKARLDALRQLAPRQQQTLIEKLQQHGITRRDFMKWSAMVTGMLALPLPFSNLVAEAAELADRVPLIWLH
MAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAGWQAEENLEHALETYKGRYLLAVEGAVPTANNGSFLTVGCK
GHTGLEIIKHAAEDAAAIISVGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCMGC
SEPNFWDDMADFEKPLGRQLLHGLDATADTVGAVILGATVVGIGAHAVASIFAKPLEE

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=333, Percent_Identity=43.8438438438438, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI1789371, Length=292, Percent_Identity=49.3150684931507, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 41072; Mature: 41072

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTHAALYEQGKARLDALRQLAPRQQQTLIEKLQQHGITRRDFMKWSAMVTGMLALPLPF
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
SNLVAEAAELADRVPLIWLHMAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAG
HHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLEHALETYKGRYLLAVEGAVPTANNGSFLTVGCKGHTGLEIIKHAAEDAAAIIS
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
VGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
HHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCMGCSEPNFWDDMADFEKPLGRQLLHGLDATADTVGAVILGATV
HHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VGIGAHAVASIFAKPLEE
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTHAALYEQGKARLDALRQLAPRQQQTLIEKLQQHGITRRDFMKWSAMVTGMLALPLPF
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
SNLVAEAAELADRVPLIWLHMAECTGCSESLVRADTPNLDSLIFDHISLEYHETLMAAAG
HHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLEHALETYKGRYLLAVEGAVPTANNGSFLTVGCKGHTGLEIIKHAAEDAAAIIS
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
VGTCASFGGVQAAYPNPTGAKGVHEVVSKPVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
HHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDHGAKEGYCLYKVGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCMGCSEPNFWDDMADFEKPLGRQLLHGLDATADTVGAVILGATV
HHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VGIGAHAVASIFAKPLEE
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]