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The map label for this gene is hydB [H]

Identifier: 117920324

GI number: 117920324

Start: 2225805

End: 2227508

Strand: Reverse

Name: hydB [H]

Synonym: Shewana3_1879

Alternate gene names: 117920324

Gene position: 2227508-2225805 (Counterclockwise)

Preceding gene: 117920325

Following gene: 117920323

Centisome position: 44.8

GC content: 52.17

Gene sequence:

>1704_bases
ATGAGCAAGCGCGTTGTTATCGACCCTATCACCCGTATTGAGGGTCATTTACGTATCGAAGTCGAAGTTGATGAGAATAA
TGTCATCAACAAAGCATGGTCATCCTCTACCCTGTGGCGTGGTATTGAAGTCATCCTCAAGGGCCGCACACCAATGGACG
TGGGGCTGATCGTGCAGCGGATCTGCGGTGTTTGTACCTATTCCCACTATCGCTGTGGTACCGAAGCGGTGGAAAATGCC
TTAGGCATTCAAATCCCGTTAAACGCTAAATACCTACGTTCCTTAATGCAAACCTCGCTCTATATGCATGACCATATAGT
GCACTTCTATCATCTACACGGTTTAGACTGGGTGGATGTTGTGTCAGCTTTAAGTGCCGATCCCGCCAAGGCTGCACAGG
TTGCCCTTAAATATACCGACAAGCCGATTGCTGCGGGCGAAGGCGAATTAAGAGCGGTGCAAGAGCGGGTTAAAGGCTTT
GTCGAAACTGGCAAACTCGGCCCCTTCGCTAACGCTTACTGGGGCAATGGCACCTATAAATTTACCCCAGAGCAAAACCT
GATTGCCCTGTCGCATTACCTCAAAGCCCTTGAAGTACAAAGGGTTGCCGCCGAAATGCTGGCGATTTTCGGGGGTAAAC
AGCCGCATCCACAATCATTGGTTGTGGGCGGTGTGACCTCAGTGCGCGATATGTTAAGCCCTGCGCGCCTGCAGGAGTGG
AAACAAAAACACGCGATTGTGACTGACTTTATTCTGCGTGCCTATCAAGCAGATATCGTCATGGCGGCGGAAGCCTTTGG
TGGTGAAGCCAGCGTACTTGGCGGCGTGAACGTTAAAAACTTCATGGCGACCAATGACTTTGTGCTTGCCAATGGCGAAT
ACCTGTTCGACCAAGGCGTGATTATGAAGGGCGACTTAGCGGGCGTAAGCGACATCAACCCAGAGCTAATCGCCGAAGAC
GTCAGCCATGCCTGGTATAGCGCAGATGCGCCGCAGCATCCCTACGATGGCACAACTATCCCCAACTACACCGGCTTTGT
TGAGCGCGATACGGTTTATGGCAAACTGCCCACCTTAGATGGCGATGGCAAATACTCTTGGGTGAAATCGCCACGTTACC
AGGGCGAACCCGTGGAAGTGGGTCCATTGTCCTGCCTGCTGGTGAGCTATGCCCGTGGCAATAAAGTGGTGGTCGATGCC
GTCAATGCGCTGCTGGCTCGCACGGGTCTGCCGGTTGAAGCCCTGTTCACCACCTTAGGCCGTACCGCAGCGCGGATGCT
GCAAACCGTTATCGTGGCGCAGGAAGGTCTGAAAACCTTCGATGCCCTGTTAACCAATATTCAATCCGATGAAACCACCT
ATGTGAAACCCCATATCGACCCAAGTCGTGAATATGTGGGTCACGCCATGATTGAAGCACCACGCGGCATGTTAAGCCAC
TGGATCCGCATCAAAAACGGCGTCATTGAGAACTATCAAGCCGTGGTGCCCACCACTTGGAACGCAGGCCCAGTGGATGC
CAATGGCAAGATTGGCCCCTACGAAGCCTCACTCATTGGTTTGAAACTGGAAGATCCCACTAAGCCCCTTGAGGTGATAC
GTATTATTCACTCATTCGACCCTTGCATGGCGTGTTCAGTACACGTGATGGATTTCAAAGGTCAAGCACTGAGCGAGTTC
CGTGTCAGCCCCAATGGTCAATAA

Upstream 100 bases:

>100_bases
CGCAGACACAGTCGGTGCGGTGATTTTAGGCGCGACCGTCGTCGGCATTGGAGCCCACGCCGTCGCCAGTATTTTTGCCA
AGCCGCTGGAGGAATAACCC

Downstream 100 bases:

>100_bases
CAACGAGGGAGGGAACGCAACATGAACCATTCTGAAACCCGCATTCGGACACTGGTTTTTAGTCCCGCGATACGGATTTT
TCACTGGCTACGGGCACTGT

Product: nickel-dependent hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSKRVVIDPITRIEGHLRIEVEVDENNVINKAWSSSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENA
LGIQIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDVVSALSADPAKAAQVALKYTDKPIAAGEGELRAVQERVKGF
VETGKLGPFANAYWGNGTYKFTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKQPHPQSLVVGGVTSVRDMLSPARLQEW
KQKHAIVTDFILRAYQADIVMAAEAFGGEASVLGGVNVKNFMATNDFVLANGEYLFDQGVIMKGDLAGVSDINPELIAED
VSHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLDGDGKYSWVKSPRYQGEPVEVGPLSCLLVSYARGNKVVVDA
VNALLARTGLPVEALFTTLGRTAARMLQTVIVAQEGLKTFDALLTNIQSDETTYVKPHIDPSREYVGHAMIEAPRGMLSH
WIRIKNGVIENYQAVVPTTWNAGPVDANGKIGPYEASLIGLKLEDPTKPLEVIRIIHSFDPCMACSVHVMDFKGQALSEF
RVSPNGQ

Sequences:

>Translated_567_residues
MSKRVVIDPITRIEGHLRIEVEVDENNVINKAWSSSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENA
LGIQIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDVVSALSADPAKAAQVALKYTDKPIAAGEGELRAVQERVKGF
VETGKLGPFANAYWGNGTYKFTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKQPHPQSLVVGGVTSVRDMLSPARLQEW
KQKHAIVTDFILRAYQADIVMAAEAFGGEASVLGGVNVKNFMATNDFVLANGEYLFDQGVIMKGDLAGVSDINPELIAED
VSHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLDGDGKYSWVKSPRYQGEPVEVGPLSCLLVSYARGNKVVVDA
VNALLARTGLPVEALFTTLGRTAARMLQTVIVAQEGLKTFDALLTNIQSDETTYVKPHIDPSREYVGHAMIEAPRGMLSH
WIRIKNGVIENYQAVVPTTWNAGPVDANGKIGPYEASLIGLKLEDPTKPLEVIRIIHSFDPCMACSVHVMDFKGQALSEF
RVSPNGQ
>Mature_566_residues
SKRVVIDPITRIEGHLRIEVEVDENNVINKAWSSSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENAL
GIQIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDVVSALSADPAKAAQVALKYTDKPIAAGEGELRAVQERVKGFV
ETGKLGPFANAYWGNGTYKFTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKQPHPQSLVVGGVTSVRDMLSPARLQEWK
QKHAIVTDFILRAYQADIVMAAEAFGGEASVLGGVNVKNFMATNDFVLANGEYLFDQGVIMKGDLAGVSDINPELIAEDV
SHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLDGDGKYSWVKSPRYQGEPVEVGPLSCLLVSYARGNKVVVDAV
NALLARTGLPVEALFTTLGRTAARMLQTVIVAQEGLKTFDALLTNIQSDETTYVKPHIDPSREYVGHAMIEAPRGMLSHW
IRIKNGVIENYQAVVPTTWNAGPVDANGKIGPYEASLIGLKLEDPTKPLEVIRIIHSFDPCMACSVHVMDFKGQALSEFR
VSPNGQ

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=583, Percent_Identity=45.4545454545455, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1787207, Length=589, Percent_Identity=39.8981324278438, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 62287; Mature: 62156

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRVVIDPITRIEGHLRIEVEVDENNVINKAWSSSTLWRGIEVILKGRTPMDVGLIVQR
CCCCEEECCHHCCCCEEEEEEEECCCCCCHHHCCCHHHHHHHHHEEECCCCCHHHHHHHH
ICGVCTYSHYRCGTEAVENALGIQIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDV
HHHHHCCCHHCCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
VSALSADPAKAAQVALKYTDKPIAAGEGELRAVQERVKGFVETGKLGPFANAYWGNGTYK
HHHHCCCCHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEE
FTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKQPHPQSLVVGGVTSVRDMLSPARLQEW
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHCHHHHHHH
KQKHAIVTDFILRAYQADIVMAAEAFGGEASVLGGVNVKNFMATNDFVLANGEYLFDQGV
HHHHHHHHHHHHHHHHHHHEEEEHHCCCCEEEECCCCHHHEEECCCEEEECCCEEECCCE
IMKGDLAGVSDINPELIAEDVSHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLD
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCC
GDGKYSWVKSPRYQGEPVEVGPLSCLLVSYARGNKVVVDAVNALLARTGLPVEALFTTLG
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCCCHHHHHHHHH
RTAARMLQTVIVAQEGLKTFDALLTNIQSDETTYVKPHIDPSREYVGHAMIEAPRGMLSH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHH
WIRIKNGVIENYQAVVPTTWNAGPVDANGKIGPYEASLIGLKLEDPTKPLEVIRIIHSFD
HHHHHCCHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCC
PCMACSVHVMDFKGQALSEFRVSPNGQ
CHHEEEEEEEECCCCCHHHCCCCCCCC
>Mature Secondary Structure 
SKRVVIDPITRIEGHLRIEVEVDENNVINKAWSSSTLWRGIEVILKGRTPMDVGLIVQR
CCCEEECCHHCCCCEEEEEEEECCCCCCHHHCCCHHHHHHHHHEEECCCCCHHHHHHHH
ICGVCTYSHYRCGTEAVENALGIQIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDV
HHHHHCCCHHCCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
VSALSADPAKAAQVALKYTDKPIAAGEGELRAVQERVKGFVETGKLGPFANAYWGNGTYK
HHHHCCCCHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEE
FTPEQNLIALSHYLKALEVQRVAAEMLAIFGGKQPHPQSLVVGGVTSVRDMLSPARLQEW
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHCHHHHHHH
KQKHAIVTDFILRAYQADIVMAAEAFGGEASVLGGVNVKNFMATNDFVLANGEYLFDQGV
HHHHHHHHHHHHHHHHHHHEEEEHHCCCCEEEECCCCHHHEEECCCEEEECCCEEECCCE
IMKGDLAGVSDINPELIAEDVSHAWYSADAPQHPYDGTTIPNYTGFVERDTVYGKLPTLD
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCC
GDGKYSWVKSPRYQGEPVEVGPLSCLLVSYARGNKVVVDAVNALLARTGLPVEALFTTLG
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCCCHHHHHHHHH
RTAARMLQTVIVAQEGLKTFDALLTNIQSDETTYVKPHIDPSREYVGHAMIEAPRGMLSH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHH
WIRIKNGVIENYQAVVPTTWNAGPVDANGKIGPYEASLIGLKLEDPTKPLEVIRIIHSFD
HHHHHCCHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCC
PCMACSVHVMDFKGQALSEFRVSPNGQ
CHHEEEEEEEECCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]