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The map label for this gene is cspD [H]

Identifier: 117920198

GI number: 117920198

Start: 2066259

End: 2066465

Strand: Reverse

Name: cspD [H]

Synonym: Shewana3_1751

Alternate gene names: 117920198

Gene position: 2066465-2066259 (Counterclockwise)

Preceding gene: 117920201

Following gene: 117920196

Centisome position: 41.56

GC content: 43.48

Gene sequence:

>207_bases
ATGGCAAGCGGAACTGTTAAATGGTTCAACAACGCCAAAGGATTCGGGTTTATTTGCCCTGATCAAGGTGGCGAGGACGT
TTTTGCACACTATTCTACCATTGAAATGGAAGGCTATAGAACTCTAAAAGCAGGCCAACCAGTTCAATTCGAAGTAGAAC
AGGGTCCAAAAGGAATGCACGCTTCAGCCATTTCACCAACAAAATAA

Upstream 100 bases:

>100_bases
CTTTTACTCCTTACATTCTGTAGCTCAGACGGTGAAACAATCCCGTCTATACAGGTTTTACTATCTTACTGGTAAGTAGA
CAACAGAAGGAAGTGGAAGT

Downstream 100 bases:

>100_bases
CGTTTGGTGAGGCCAATGATCTAAAAGGCAGGGATTATATCCCTGCCTTTTTATTGGGCGATTGACAACTTTTCTCAAAG
ATTGAGTCTGATAAAAAAGG

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: CSP-D [H]

Number of amino acids: Translated: 68; Mature: 67

Protein sequence:

>68_residues
MASGTVKWFNNAKGFGFICPDQGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMHASAISPTK

Sequences:

>Translated_68_residues
MASGTVKWFNNAKGFGFICPDQGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMHASAISPTK
>Mature_67_residues
ASGTVKWFNNAKGFGFICPDQGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMHASAISPTK

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=68, Percent_Identity=77.9411764705882, Blast_Score=118, Evalue=9e-29,
Organism=Escherichia coli, GI1789979, Length=63, Percent_Identity=52.3809523809524, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=52.4590163934426, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=50, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787839, Length=65, Percent_Identity=52.3076923076923, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=51.6129032258064, Blast_Score=72, Evalue=5e-15,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=49.2307692307692, Blast_Score=70, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24658520, Length=62, Percent_Identity=45.1612903225806, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7381; Mature: 7250

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGTVKWFNNAKGFGFICPDQGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMH
CCCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCCEEEEECCCCCCCC
ASAISPTK
CCCCCCCC
>Mature Secondary Structure 
ASGTVKWFNNAKGFGFICPDQGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMH
CCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCCEEEEECCCCCCCC
ASAISPTK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]