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The map label for this gene is rluE [H]

Identifier: 117920196

GI number: 117920196

Start: 2062723

End: 2063532

Strand: Reverse

Name: rluE [H]

Synonym: Shewana3_1749

Alternate gene names: 117920196

Gene position: 2063532-2062723 (Counterclockwise)

Preceding gene: 117920198

Following gene: 117920195

Centisome position: 41.5

GC content: 51.48

Gene sequence:

>810_bases
ATGAACAAAAACAGCACGCCAGCAACCGCATCCAGCCCACGCGCCAGCGGCAAACGCCCCGCAACAGGGCGCGCCAATCC
GGCGGATAACCGCAATAGCGCCAAGAAACCCCATACCTTCGCCAAGAATGCCAAGCCAAGTTTACAGCAAGGGGCTAATT
CTGAGAGACACAGTGCCAAGCGAAACAGTGCTGAGCGCAGCGGCGCCAAGTCTCGCCCGCAACAGCGCTCAACGGCTGCA
CGCACAAAACCCCAAGGCGAGCCGATGATCGTCTTATTTAATAAGCCGTTCGATGTCCTATGCCAATTTACCGATGAGCA
AGGCCGTAAAACCTTAAAGGATTACATCCCGATTCCGGGAGTGTACGCTGCAGGACGATTGGATCGCGACAGTGAAGGCT
TGTTACTCCTCACCAACGATGGTCAACTTCAGGCCAAACTCACTGAGCCGAAAAAGAAAACCTTTAAAACCTATTGGGTG
CAAGTCGAGGGAGAACCCACGGATGCGGATTTAGCAAAATTGCGCCAAGGGGTTGAATTAAAAGATGGTATAACACTCCC
CGCTAAGGTCAGCATCATGGCGACGCCGAATGTTTGGCCGCGTAATCCGCCGATTCGTGAGCGAAAAAACATCCCTACCA
CTTGGCTGGAGATTCAAATCCATGAAGGACGCAATCGCCAAGTTCGGCGGATGACGGCCCATATTGGTTTTCCAACCTTA
AGACTCATTCGTTATAAAATAGGCCAATGGAGTCTAGATGATTTAGCCACGGGTGAGCATAAATGCATCCAAGTGACTCA
AAAGGCTTAA

Upstream 100 bases:

>100_bases
CACTAACGGCGAATGCCAAGTACACTGTGTACAGATTATTGATACACGTCCTCAAACTTTTAAGTTCGGTCGTCAGCTTA
CAGCGACTATTAGCCACAGC

Downstream 100 bases:

>100_bases
TGATTTAACAACACTGCGAATGCAATAAACAGGATGTCATTGTGCCCAGCCAACAGATAAAAAGGTGATTAGCGTGGCAG
AAAAACTCAGATACAAACCC

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNKNSTPATASSPRASGKRPATGRANPADNRNSAKKPHTFAKNAKPSLQQGANSERHSAKRNSAERSGAKSRPQQRSTAA
RTKPQGEPMIVLFNKPFDVLCQFTDEQGRKTLKDYIPIPGVYAAGRLDRDSEGLLLLTNDGQLQAKLTEPKKKTFKTYWV
QVEGEPTDADLAKLRQGVELKDGITLPAKVSIMATPNVWPRNPPIRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPTL
RLIRYKIGQWSLDDLATGEHKCIQVTQKA

Sequences:

>Translated_269_residues
MNKNSTPATASSPRASGKRPATGRANPADNRNSAKKPHTFAKNAKPSLQQGANSERHSAKRNSAERSGAKSRPQQRSTAA
RTKPQGEPMIVLFNKPFDVLCQFTDEQGRKTLKDYIPIPGVYAAGRLDRDSEGLLLLTNDGQLQAKLTEPKKKTFKTYWV
QVEGEPTDADLAKLRQGVELKDGITLPAKVSIMATPNVWPRNPPIRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPTL
RLIRYKIGQWSLDDLATGEHKCIQVTQKA
>Mature_269_residues
MNKNSTPATASSPRASGKRPATGRANPADNRNSAKKPHTFAKNAKPSLQQGANSERHSAKRNSAERSGAKSRPQQRSTAA
RTKPQGEPMIVLFNKPFDVLCQFTDEQGRKTLKDYIPIPGVYAAGRLDRDSEGLLLLTNDGQLQAKLTEPKKKTFKTYWV
QVEGEPTDADLAKLRQGVELKDGITLPAKVSIMATPNVWPRNPPIRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPTL
RLIRYKIGQWSLDDLATGEHKCIQVTQKA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=216, Percent_Identity=60.1851851851852, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1787524, Length=184, Percent_Identity=31.5217391304348, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788510, Length=183, Percent_Identity=33.879781420765, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790453, Length=172, Percent_Identity=27.906976744186, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 30033; Mature: 30033

Theoretical pI: Translated: 11.00; Mature: 11.00

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNSTPATASSPRASGKRPATGRANPADNRNSAKKPHTFAKNAKPSLQQGANSERHSAK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHCCCCHHHHHH
RNSAERSGAKSRPQQRSTAARTKPQGEPMIVLFNKPFDVLCQFTDEQGRKTLKDYIPIPG
HHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHEEECCCHHHHHHHHHHCCCCC
VYAAGRLDRDSEGLLLLTNDGQLQAKLTEPKKKTFKTYWVQVEGEPTDADLAKLRQGVEL
EEECCCCCCCCCCEEEEECCCEEEEEECCCHHHEEEEEEEEEECCCCCHHHHHHHCCCCC
KDGITLPAKVSIMATPNVWPRNPPIRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPTL
CCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHH
RLIRYKIGQWSLDDLATGEHKCIQVTQKA
HHHHHHCCCCCHHHHCCCCCEEEEEECCC
>Mature Secondary Structure
MNKNSTPATASSPRASGKRPATGRANPADNRNSAKKPHTFAKNAKPSLQQGANSERHSAK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHCCCCHHHHHH
RNSAERSGAKSRPQQRSTAARTKPQGEPMIVLFNKPFDVLCQFTDEQGRKTLKDYIPIPG
HHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHEEECCCHHHHHHHHHHCCCCC
VYAAGRLDRDSEGLLLLTNDGQLQAKLTEPKKKTFKTYWVQVEGEPTDADLAKLRQGVEL
EEECCCCCCCCCCEEEEECCCEEEEEECCCHHHEEEEEEEEEECCCCCHHHHHHHCCCCC
KDGITLPAKVSIMATPNVWPRNPPIRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPTL
CCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHH
RLIRYKIGQWSLDDLATGEHKCIQVTQKA
HHHHHHCCCCCHHHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]