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The map label for this gene is sucB [H]

Identifier: 117920158

GI number: 117920158

Start: 2015520

End: 2016713

Strand: Direct

Name: sucB [H]

Synonym: Shewana3_1711

Alternate gene names: 117920158

Gene position: 2015520-2016713 (Clockwise)

Preceding gene: 117920157

Following gene: 117920159

Centisome position: 40.54

GC content: 48.66

Gene sequence:

>1194_bases
ATGAGTATCGAAATCAAGGTACCCGTACTGCCAGAATCTGTTGCCGATGCAACTATCGCCACTTGGCACGTAAAAGTGGG
TGAGCAAGTTTCTCGTGATCAAAATCTGGTTGATATTGAAACTGACAAAGTCGTACTGGAAGTGGTTGCACCAGAAGATG
GTCACATCGGTGAGTTCCTGTTCCAAGAAGGCGACACCGTTCTGGGCGAGCAAGTGATTGCTAAGTTCATCGCGGGTGCG
GTTTCTGGTCAAGAAGTGACTAAAGCCGAAGCTGAAGCTGCTGCACCTGTTGCCGCTGCTGCAACTGAAGAGTCTAACGA
TGCATTAAGCCCATCAGTACGTCGTTTACTGGCTGAGCACAATGTTGATGCGAGCAAAGTGAAGGGCACTGGTGTCGGTG
GCCGTATCACTAAAGAAGACGTTGAAGCCTTTATCAAGTCTGCGCCTAAAGCTGCTGCCCCTGCGGCTCCTGCGGTACAA
CCACTCGCTGCGGGTCGTAGCGAGAAACGTGTTCCAATGACTCGTTTACGTAAGACTATCGCTAACCGTTTATTAGAAGC
GAAAAACTCTACTGCAATGCTCACGACGTTCAACGAAGTGAACATGAAGCCAATCATGGATATCCGTAAGCAATATCAAG
ATATCTTTGAAAAGCGCCACGGTATCCGTTTAGGTTTCATGTCTTTCTACGTGAAAGCGGTAACCGAAGCGCTGAAACGC
TTCCCAGAAGTAAACGCGTCAATCGACGGTGATGATATTGTTTACCACAACTACTTCGACGTCAGCATCGCGGTATCGAC
TCCTCGTGGTCTGGTCACTCCAGTATTGCGTGATACCGATACTATGAGCCTAGCTGATATCGAAAAAGCAGTCCGCGATC
TGGCGATCAAAGGCCGTGATGGCAAGCTGACTGTGGCTGATATGACTGGCGGTAACTTCACAGTGACTAACGGTGGTGTA
TTCGGCTCGTTAATGTCTACCCCAATTTTAAACCTGCCACAAAGCGCGATTTTAGGCATGCACGCCATTAAAGACCGCCC
AATGGCAGTCAATGGTCAGGTTGAAATCCTGCCCATGATGTACCTAGCTCTCTCATATGACCATCGTATTGTCGATGGCC
GTGAGTCAGTTGGCTTCTTAGTCGCCATTAAAGACTTCTTGGAAGACCCAACTCGTCTGCTACTTGACCTATAA

Upstream 100 bases:

>100_bases
GCTGCATGCTCACCAACAAGAATCTTTAGTGAACAGTGCCCTAAAGCTGTAATAGCTAAGACTGTAGTAATAGACATTTT
ATAAAAAAGGATCGTTTTTC

Downstream 100 bases:

>100_bases
TCTAATGCGCTAAACGATAAAGTTTAGCCTGTTAGTTAAAGACAAGCGTCAGACATACCCTCGTGACTGGCCCCGTGGGG
CCAGTTGGATTTGATTAGAA

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFLFQEGDTVLGEQVIAKFIAGA
VSGQEVTKAEAEAAAPVAAAATEESNDALSPSVRRLLAEHNVDASKVKGTGVGGRITKEDVEAFIKSAPKAAAPAAPAVQ
PLAAGRSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEALKR
FPEVNASIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIKGRDGKLTVADMTGGNFTVTNGGV
FGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL

Sequences:

>Translated_397_residues
MSIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFLFQEGDTVLGEQVIAKFIAGA
VSGQEVTKAEAEAAAPVAAAATEESNDALSPSVRRLLAEHNVDASKVKGTGVGGRITKEDVEAFIKSAPKAAAPAAPAVQ
PLAAGRSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEALKR
FPEVNASIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIKGRDGKLTVADMTGGNFTVTNGGV
FGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL
>Mature_396_residues
SIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFLFQEGDTVLGEQVIAKFIAGAV
SGQEVTKAEAEAAAPVAAAATEESNDALSPSVRRLLAEHNVDASKVKGTGVGGRITKEDVEAFIKSAPKAAAPAAPAVQP
LAAGRSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEALKRF
PEVNASIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIKGRDGKLTVADMTGGNFTVTNGGVF
GSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=247, Percent_Identity=54.6558704453441, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=30.8045977011494, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI110671329, Length=426, Percent_Identity=26.5258215962441, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=27.212389380531, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=27.212389380531, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=33.5403726708075, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=72.4565756823821, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=33.9066339066339, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=408, Percent_Identity=41.9117647058824, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=31.651376146789, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=25.7075471698113, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=28.8461538461538, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=400, Percent_Identity=42.75, Blast_Score=317, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=25.3897550111359, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24645909, Length=237, Percent_Identity=58.6497890295359, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI18859875, Length=416, Percent_Identity=29.3269230769231, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI20129315, Length=290, Percent_Identity=33.448275862069, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42963; Mature: 42832

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFL
CEEEEEECCCCCHHCCCEEEEEEEECCHHHHCCCCEEEEECCEEEEEEECCCCCCHHHHH
FQEGDTVLGEQVIAKFIAGAVSGQEVTKAEAEAAAPVAAAATEESNDALSPSVRRLLAEH
HHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHC
NVDASKVKGTGVGGRITKEDVEAFIKSAPKAAAPAAPAVQPLAAGRSEKRVPMTRLRKTI
CCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHH
ANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEALKR
HHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
FPEVNASIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIKGRD
CCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHEECCCC
GKLTVADMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMM
CEEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHHHHH
YLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL
HHHHCCCCEEECCCCCCCHHEEHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFL
EEEEEECCCCCHHCCCEEEEEEEECCHHHHCCCCEEEEECCEEEEEEECCCCCCHHHHH
FQEGDTVLGEQVIAKFIAGAVSGQEVTKAEAEAAAPVAAAATEESNDALSPSVRRLLAEH
HHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHC
NVDASKVKGTGVGGRITKEDVEAFIKSAPKAAAPAAPAVQPLAAGRSEKRVPMTRLRKTI
CCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHH
ANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYVKAVTEALKR
HHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
FPEVNASIDGDDIVYHNYFDVSIAVSTPRGLVTPVLRDTDTMSLADIEKAVRDLAIKGRD
CCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHEECCCC
GKLTVADMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMM
CEEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHHHHH
YLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL
HHHHCCCCEEECCCCCCCHHEEHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]