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The map label for this gene is mvaB [H]

Identifier: 117920115

GI number: 117920115

Start: 1959088

End: 1960020

Strand: Reverse

Name: mvaB [H]

Synonym: Shewana3_1668

Alternate gene names: 117920115

Gene position: 1960020-1959088 (Counterclockwise)

Preceding gene: 117920116

Following gene: 117920114

Centisome position: 39.42

GC content: 53.16

Gene sequence:

>933_bases
ATGTCTGCGCTGGATCTTTCGACCTTAAGCGCCAGCTCGGATAGGGTTAGCCTATTCGAGATGGGGCCCCGTGATGGCTT
GCAGAATGAGGCCGCCGTCCCAACACAGGCCAAAGTCGCCTTGATTGAATCCCTCGCCGATGCGGGCGTGAAGCGAATTG
AAGCAGGCAGTTTTGTGTCACCCAAATGGGTGCCACAAATGGCCGATAGCGGTGACGTATTGCGCCAAATTCGCCGTCAA
GCGGGCGTGGTTTACAGCGCCCTCACCCCTAATGTCAAAGGCTTAGAACTGGCGCTGGATGCAAAAGCTTCCGAAGTCGC
TATCTTTGGTGCGGCGTCACAAAGCTTTAGCCAACGCAATATCAACTGTTCGATTGAAGAGTCGATTGAACGCTTTATCC
CGTTGATGGACATGGCAAAAGCCGCCAATATCCCAGTGCGTGGCTATGTGTCCTGCGTTTTAGGTTGCCCCTATGAAGGG
GAAATCGCGGCGAGCGAAGTGGCAAGAGTGTCTGAAATCCTTTACAAAATGGGCTGTTACGAAATTTCGCTCGGCGACAC
TATCGGTGTAGGCACGCCGTTAAAAGCCCGTAAAATGCTGCAGGCCGTGATGGAGCGAGTCCCGGTTGAAATGCTCGCCT
TGCACTTTCACGACACCTATGGCCAAGCATTAGCCAATATTACCGCCTGCCTCGACTTAGGTGTGCGCAGCTTTGACGCC
TCAGTGGCGGGTCTTGGTGGCTGCCCGTATGCAAAAGGCGCGTCGGGCAATCTGGCGAGTGAGGACTTGGTCTATATGCT
CCACGGACTCGGTTTGAAGACGGGTATCGATTTAGAAAAACTGGCATTAGCGGGTTTTGGGATCAGCAAACAATTAAATC
GTCTTAATGGCTCTAAAGTCGCCAATGCAATCTTGCAGGGCGCCAAAGCCTAG

Upstream 100 bases:

>100_bases
CGGTATCGTTACCGAGTTCTACTTTAAGGCGGGCGAGTTAGTGAGCGACGGCGCTGTGCTGCTCCATGTTGAGCCCAAAG
CCCAGAGCGAGGAAGCCTAA

Downstream 100 bases:

>100_bases
ACTCAAGCGTCGAGGCGCTCAACATACCTAGCGCCTTGGGGACTGAATAACAGTAAAAGGACATCAAAATGGCAGGACTC
AATAAAGTCGTCAGCAGCTA

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSALDLSTLSASSDRVSLFEMGPRDGLQNEAAVPTQAKVALIESLADAGVKRIEAGSFVSPKWVPQMADSGDVLRQIRRQ
AGVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRNINCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEG
EIAASEVARVSEILYKMGCYEISLGDTIGVGTPLKARKMLQAVMERVPVEMLALHFHDTYGQALANITACLDLGVRSFDA
SVAGLGGCPYAKGASGNLASEDLVYMLHGLGLKTGIDLEKLALAGFGISKQLNRLNGSKVANAILQGAKA

Sequences:

>Translated_310_residues
MSALDLSTLSASSDRVSLFEMGPRDGLQNEAAVPTQAKVALIESLADAGVKRIEAGSFVSPKWVPQMADSGDVLRQIRRQ
AGVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRNINCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEG
EIAASEVARVSEILYKMGCYEISLGDTIGVGTPLKARKMLQAVMERVPVEMLALHFHDTYGQALANITACLDLGVRSFDA
SVAGLGGCPYAKGASGNLASEDLVYMLHGLGLKTGIDLEKLALAGFGISKQLNRLNGSKVANAILQGAKA
>Mature_309_residues
SALDLSTLSASSDRVSLFEMGPRDGLQNEAAVPTQAKVALIESLADAGVKRIEAGSFVSPKWVPQMADSGDVLRQIRRQA
GVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRNINCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEGE
IAASEVARVSEILYKMGCYEISLGDTIGVGTPLKARKMLQAVMERVPVEMLALHFHDTYGQALANITACLDLGVRSFDAS
VAGLGGCPYAKGASGNLASEDLVYMLHGLGLKTGIDLEKLALAGFGISKQLNRLNGSKVANAILQGAKA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=304, Percent_Identity=58.8815789473684, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI109150422, Length=302, Percent_Identity=55.9602649006622, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI109150427, Length=300, Percent_Identity=56, Blast_Score=349, Evalue=2e-96,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=64.7058823529412, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1786261, Length=249, Percent_Identity=26.5060240963855, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17510563, Length=274, Percent_Identity=52.9197080291971, Blast_Score=304, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24582381, Length=305, Percent_Identity=55.7377049180328, Blast_Score=353, Evalue=7e-98,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 32685; Mature: 32554

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALDLSTLSASSDRVSLFEMGPRDGLQNEAAVPTQAKVALIESLADAGVKRIEAGSFVS
CCCCCCCHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PKWVPQMADSGDVLRQIRRQAGVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRN
CCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHCC
INCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEGEIAASEVARVSEILYKMGCY
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCE
EISLGDTIGVGTPLKARKMLQAVMERVPVEMLALHFHDTYGQALANITACLDLGVRSFDA
EEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SVAGLGGCPYAKGASGNLASEDLVYMLHGLGLKTGIDLEKLALAGFGISKQLNRLNGSKV
CHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHH
ANAILQGAKA
HHHHHHCCCC
>Mature Secondary Structure 
SALDLSTLSASSDRVSLFEMGPRDGLQNEAAVPTQAKVALIESLADAGVKRIEAGSFVS
CCCCCCHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PKWVPQMADSGDVLRQIRRQAGVVYSALTPNVKGLELALDAKASEVAIFGAASQSFSQRN
CCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHCC
INCSIEESIERFIPLMDMAKAANIPVRGYVSCVLGCPYEGEIAASEVARVSEILYKMGCY
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCE
EISLGDTIGVGTPLKARKMLQAVMERVPVEMLALHFHDTYGQALANITACLDLGVRSFDA
EEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SVAGLGGCPYAKGASGNLASEDLVYMLHGLGLKTGIDLEKLALAGFGISKQLNRLNGSKV
CHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHH
ANAILQGAKA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]