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The map label for this gene is trpA [H]

Identifier: 117919963

GI number: 117919963

Start: 1765029

End: 1765865

Strand: Reverse

Name: trpA [H]

Synonym: Shewana3_1515

Alternate gene names: 117919963

Gene position: 1765865-1765029 (Counterclockwise)

Preceding gene: 117919964

Following gene: 117919961

Centisome position: 35.51

GC content: 54.36

Gene sequence:

>837_bases
ATGAGCAACACTGCAACCCAAACTATCCCTCAGCAAGGCGGCCGTTATCACGCAGCGTTTAGCCGTTTAAAAGCCGAAGG
CCGCGGCGCCTTTGTGCCCTTTGTCACTTTGGGCGATCCAAGCCCTGAGCTGTCGCTGAAAATTATCGATACCTTAGTGC
AAAATGGTGCCGATGCTCTGGAATTAGGTTTTCCCTTCTCCGATCCGCTAGCAGACGGCCCGGTGATCCAAGGCGCGAAC
CTCAGAGCCCTCGCCGCGGGTACGACGCCAACGCAGTGCTTCAACTTATTAGCGCAAATTCGCGCTAAATACCCAGAGCT
GCCGATTGGATTATTGCTGTACGCGAACCTGGTATTCGCTAATGGCATCGATGCTTTTTATGCCAAAGCGCAGCAAGCGG
GCGTGGACTCAGTGCTTATCGCCGATGTGCCAGTAGAAGAAGCGGCGCCTTTTATTCAAGCCGCTAAGGCCCACGGTATC
GCGCCGATTTTTATCGCGCCGCCCAATGCCGATAGCGAAACCCTTGAAAAAGTGAGCCAAAGTGGCGAAGGCTATACCTA
TCTATTGTCCCGCGCCGGTGTCACTGGCACAGACACTAAGGCGGGTACGCCCGTTGAAGAAATTTTAGCTAAGCTGCAAG
CATTCAATGCGCCGCCACCGCTATTAGGCTTTGGGATTGCCGAGCCAGCTCAAGTGAGCGCCGCAATCAAGGCGGGCGCA
GCGGGCGCGATTTCTGGCTCGGCCGTGGTGAAAATTATCGAAGCTCATCAACAGGATGAAGCTAAGTTGCTGTCCACTTT
AGGTGAATTTACCCGCGCCATGAAAGCGGCAACCTAA

Upstream 100 bases:

>100_bases
GCACTAAAGAAACCATTTTGGTCGTTAACCTCTCGGGACGAGGCGATAAGGATATTTTCACTGTGTCAGACATTTTAAAC
GGTAAAGAGGAATAACAACG

Downstream 100 bases:

>100_bases
TTAAACTACTGACCATCTGAGCATAGGCGTGTTTGCACCTGTGCTCAGTTGTTTCTAAGCGCTAAAAATGCCTCCAGCTC
AGTTCGACAATCAGGTCTAC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSNTATQTIPQQGGRYHAAFSRLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADALELGFPFSDPLADGPVIQGAN
LRALAAGTTPTQCFNLLAQIRAKYPELPIGLLLYANLVFANGIDAFYAKAQQAGVDSVLIADVPVEEAAPFIQAAKAHGI
APIFIAPPNADSETLEKVSQSGEGYTYLLSRAGVTGTDTKAGTPVEEILAKLQAFNAPPPLLGFGIAEPAQVSAAIKAGA
AGAISGSAVVKIIEAHQQDEAKLLSTLGEFTRAMKAAT

Sequences:

>Translated_278_residues
MSNTATQTIPQQGGRYHAAFSRLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADALELGFPFSDPLADGPVIQGAN
LRALAAGTTPTQCFNLLAQIRAKYPELPIGLLLYANLVFANGIDAFYAKAQQAGVDSVLIADVPVEEAAPFIQAAKAHGI
APIFIAPPNADSETLEKVSQSGEGYTYLLSRAGVTGTDTKAGTPVEEILAKLQAFNAPPPLLGFGIAEPAQVSAAIKAGA
AGAISGSAVVKIIEAHQQDEAKLLSTLGEFTRAMKAAT
>Mature_277_residues
SNTATQTIPQQGGRYHAAFSRLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADALELGFPFSDPLADGPVIQGANL
RALAAGTTPTQCFNLLAQIRAKYPELPIGLLLYANLVFANGIDAFYAKAQQAGVDSVLIADVPVEEAAPFIQAAKAHGIA
PIFIAPPNADSETLEKVSQSGEGYTYLLSRAGVTGTDTKAGTPVEEILAKLQAFNAPPPLLGFGIAEPAQVSAAIKAGAA
GAISGSAVVKIIEAHQQDEAKLLSTLGEFTRAMKAAT

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=264, Percent_Identity=65.1515151515152, Blast_Score=332, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6321412, Length=248, Percent_Identity=31.8548387096774, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 28767; Mature: 28636

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTATQTIPQQGGRYHAAFSRLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADAL
CCCCCCCHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEE
ELGFPFSDPLADGPVIQGANLRALAAGTTPTQCFNLLAQIRAKYPELPIGLLLYANLVFA
EECCCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
NGIDAFYAKAQQAGVDSVLIADVPVEEAAPFIQAAKAHGIAPIFIAPPNADSETLEKVSQ
CCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
SGEGYTYLLSRAGVTGTDTKAGTPVEEILAKLQAFNAPPPLLGFGIAEPAQVSAAIKAGA
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHCCC
AGAISGSAVVKIIEAHQQDEAKLLSTLGEFTRAMKAAT
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNTATQTIPQQGGRYHAAFSRLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADAL
CCCCCCHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEE
ELGFPFSDPLADGPVIQGANLRALAAGTTPTQCFNLLAQIRAKYPELPIGLLLYANLVFA
EECCCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
NGIDAFYAKAQQAGVDSVLIADVPVEEAAPFIQAAKAHGIAPIFIAPPNADSETLEKVSQ
CCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
SGEGYTYLLSRAGVTGTDTKAGTPVEEILAKLQAFNAPPPLLGFGIAEPAQVSAAIKAGA
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHCCC
AGAISGSAVVKIIEAHQQDEAKLLSTLGEFTRAMKAAT
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]