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The map label for this gene is mdtE [H]

Identifier: 117919895

GI number: 117919895

Start: 1678404

End: 1679417

Strand: Reverse

Name: mdtE [H]

Synonym: Shewana3_1447

Alternate gene names: 117919895

Gene position: 1679417-1678404 (Counterclockwise)

Preceding gene: 117919896

Following gene: 117919894

Centisome position: 33.78

GC content: 52.56

Gene sequence:

>1014_bases
ATGGCTGCAATCTCACGTACTCTATTCACATTATTCGTTTTTTCCCCCTTTGCCACCCAGGCTGCGCCCTTAGCGACCCT
CGAGGTGATGAGCAAACCGTACGCGAATTGGGTGACCTTAGACGCCAGTATCGAGGCTGTCAAAGCGGCTACCGTCTCGG
CGCAAACCTCAGGGCGTATCGTTAAGCTCAACTACGATGTTAATGATGTCGTGCCAGAAGGCGCGGCGTTACTCGAGATC
ACCAGCAAAGAGCAAGGCGCAGAACTGGCAAGCTATGAAGCCGATCTCGCCAAAGCAACAGCCCTGAATGTCGAGGCGCA
GGCCCAATACAAACGCTATAAAGAACTCTTCCCCCAAGGCGCGATTTCAAAAGGCGCCATGGATGAAGCGACCGCCAATG
CCAAGGCCGCCGAACAAGCCGTGAGTGCCGCCAAAGCTAGGGTTATCAAAGCCACTGAATCCCTCAAATATACTGTGGTA
TCGGCACCATTTAGCGGCATTGTCACCGAGCGTTTAGTCGAATTAGGCGAAACCGTCAGTGTCGGTCAGCCACTGCTATC
GGGGTTTTCACCGAGCCAAATGCGCGCTATCACCCAAGTGCCACAGCGCTATATTCAGCAACTGAAAAACGCGCCTGAGT
TTATGGTGCGCTTAAGTGATGGTCGTGAACTCACCTCCAAGGATCTCACCATCTTTAGCTTTGCCGACCCAGTGAGCCAC
AGCTATCAGGTACGGATTAACCTGCCAAAGGATGAGCCTAACCTTCAACCCGGTACTTGGGCCAAGGCGCTGTTTAAAAA
TGGCGAACGGGAAAAAATCCAACTGCCGACCTCGACGCTTATCACCATGAATGAGCTTAGCAGTGTCTACTTGAAAAAAG
GCGAGCAATTTGTGCTCACCCAGGTGCGCGTCTCTGAGCCCGTCGGTGGTGAAGTAGAAGTCCTCGCGGGACTGCGAACC
GGTGATATTGTCGCCATGGATGCTTACCAAGTGTTGCTTGAACAAAAGCAATAA

Upstream 100 bases:

>100_bases
GCGAAATCGATTACATTAAATTTTTCTAATAAATATTGATTGCCTAATCAAAACAACTCCATTAGAATTTACTCATGAAT
TATTTTTGGGATGATTTGGT

Downstream 100 bases:

>100_bases
TTCCCTAGCGATATCAACTTGATGAGGTTGTTGAATGAAACACGCTACTGAATCTTCGACGTCACTTGGCATTTCGGGTC
GGATTGCGGCCGCATTTCAA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAAISRTLFTLFVFSPFATQAAPLATLEVMSKPYANWVTLDASIEAVKAATVSAQTSGRIVKLNYDVNDVVPEGAALLEI
TSKEQGAELASYEADLAKATALNVEAQAQYKRYKELFPQGAISKGAMDEATANAKAAEQAVSAAKARVIKATESLKYTVV
SAPFSGIVTERLVELGETVSVGQPLLSGFSPSQMRAITQVPQRYIQQLKNAPEFMVRLSDGRELTSKDLTIFSFADPVSH
SYQVRINLPKDEPNLQPGTWAKALFKNGEREKIQLPTSTLITMNELSSVYLKKGEQFVLTQVRVSEPVGGEVEVLAGLRT
GDIVAMDAYQVLLEQKQ

Sequences:

>Translated_337_residues
MAAISRTLFTLFVFSPFATQAAPLATLEVMSKPYANWVTLDASIEAVKAATVSAQTSGRIVKLNYDVNDVVPEGAALLEI
TSKEQGAELASYEADLAKATALNVEAQAQYKRYKELFPQGAISKGAMDEATANAKAAEQAVSAAKARVIKATESLKYTVV
SAPFSGIVTERLVELGETVSVGQPLLSGFSPSQMRAITQVPQRYIQQLKNAPEFMVRLSDGRELTSKDLTIFSFADPVSH
SYQVRINLPKDEPNLQPGTWAKALFKNGEREKIQLPTSTLITMNELSSVYLKKGEQFVLTQVRVSEPVGGEVEVLAGLRT
GDIVAMDAYQVLLEQKQ
>Mature_336_residues
AAISRTLFTLFVFSPFATQAAPLATLEVMSKPYANWVTLDASIEAVKAATVSAQTSGRIVKLNYDVNDVVPEGAALLEIT
SKEQGAELASYEADLAKATALNVEAQAQYKRYKELFPQGAISKGAMDEATANAKAAEQAVSAAKARVIKATESLKYTVVS
APFSGIVTERLVELGETVSVGQPLLSGFSPSQMRAITQVPQRYIQQLKNAPEFMVRLSDGRELTSKDLTIFSFADPVSHS
YQVRINLPKDEPNLQPGTWAKALFKNGEREKIQLPTSTLITMNELSSVYLKKGEQFVLTQVRVSEPVGGEVEVLAGLRTG
DIVAMDAYQVLLEQKQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=300, Percent_Identity=26.3333333333333, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081951, Length=197, Percent_Identity=31.9796954314721, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 36582; Mature: 36451

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAISRTLFTLFVFSPFATQAAPLATLEVMSKPYANWVTLDASIEAVKAATVSAQTSGRI
CCHHHHHHHHHHHHCCHHHCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHEEEECCCCEE
VKLNYDVNDVVPEGAALLEITSKEQGAELASYEADLAKATALNVEAQAQYKRYKELFPQG
EEEECCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCC
AISKGAMDEATANAKAAEQAVSAAKARVIKATESLKYTVVSAPFSGIVTERLVELGETVS
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCCCHH
VGQPLLSGFSPSQMRAITQVPQRYIQQLKNAPEFMVRLSDGRELTSKDLTIFSFADPVSH
CCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCEEEEEECCCCCC
SYQVRINLPKDEPNLQPGTWAKALFKNGEREKIQLPTSTLITMNELSSVYLKKGEQFVLT
CEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEECCCHHEEEHHHHHHHHHHCCCEEEEE
QVRVSEPVGGEVEVLAGLRTGDIVAMDAYQVLLEQKQ
EEEECCCCCCCEEEEECCCCCCEEEHHHHHHHHHCCC
>Mature Secondary Structure 
AAISRTLFTLFVFSPFATQAAPLATLEVMSKPYANWVTLDASIEAVKAATVSAQTSGRI
CHHHHHHHHHHHHCCHHHCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHEEEECCCCEE
VKLNYDVNDVVPEGAALLEITSKEQGAELASYEADLAKATALNVEAQAQYKRYKELFPQG
EEEECCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCC
AISKGAMDEATANAKAAEQAVSAAKARVIKATESLKYTVVSAPFSGIVTERLVELGETVS
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCCCHH
VGQPLLSGFSPSQMRAITQVPQRYIQQLKNAPEFMVRLSDGRELTSKDLTIFSFADPVSH
CCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCEEEEEECCCCCC
SYQVRINLPKDEPNLQPGTWAKALFKNGEREKIQLPTSTLITMNELSSVYLKKGEQFVLT
CEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEECCCHHEEEHHHHHHHHHHCCCEEEEE
QVRVSEPVGGEVEVLAGLRTGDIVAMDAYQVLLEQKQ
EEEECCCCCCCEEEEECCCCCCEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]