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The map label for this gene is proS [H]

Identifier: 117919846

GI number: 117919846

Start: 1613601

End: 1615313

Strand: Reverse

Name: proS [H]

Synonym: Shewana3_1398

Alternate gene names: 117919846

Gene position: 1615313-1613601 (Counterclockwise)

Preceding gene: 117919847

Following gene: 117919845

Centisome position: 32.49

GC content: 49.74

Gene sequence:

>1713_bases
ATGCGCGTTAGCAAATACCTACTTTCTACTCAAAAAGAAACACCTGCGAATGCAGAAGTCATCAGCCATCAGTTAATGCT
ACGTGCGGGTATGATCCGCCGTAACGCTTCAGGTCTATACAGCTATCTGCCCACGGGCCTGCGTGTATTGCGTAAAGTCG
AAGCCATTGTTCGTGAAGAAATGAACAAAGCGGGCGCGATTGAAATCCTAATGCCTATGGTGCAACCGGCTGATTTATGG
GTAGAAACGGGTCGCTGGGACAAATTTGGTCCTGAACTACTGCGTTTTAAAGACCGTCATAACCGTGACTTCGTGCTGGG
GCCAACCCACGAAGAAGTGATCACCGACTTAATCCGTAAAGAAGTCAGCTCTTACAAGCAATTACCGCTGAATCTTTACC
AAATCCAGACCAAATTCCGTGACGAAGTCCGCCCACGTTTCGGGGTGATGCGTTCGCGCGAATTCCTGATGAAAGATGCG
TATTCTTTCCATCTTGACGTGGATACCATGAATGAAACCTATGAGGCCATGTATCAAGCCTATAGCAACATTCTGTCGCG
CATGGGCTTAGCCTTCCGCCCTGTTTTAGCGGACACAGGTTCTATCGGTGGCAGCATGTCACACGAGTTCCATGTATTGG
CGCAAAGCGGTGAAGACTTAATCGCCTACTCTACTGGCAGCGACTATGCCGCTAACATCGAGAAAGCCGAATCGCCAATG
CCAACCGAAGCGCGTGGCGCGGCAACAGAAGCATTACGTTTAGTCGATACCCCAAATGCGAAAACCATTGCCGAGTTAGT
TGAGCAATTCGGTTTGGATATCACTAAGACAGTGAAAACCTTGATTGTGAAAGGTGCGACGGAAGAAGCACCGCTGGTAG
CGCTGATTGTACGTGGCGACCACGAGCTGAACGAAATCAAGGCAGACAAGCTAGATTTAGTGGCATCACCATTAGAATTT
GCCCCAGAAGCCCTGATCCGTGATGCCATTGGCGCAGGTCCTGGCTCATTAGGTCCTGTCGGCCTGAATATGCCTGTCAT
CATCGACCACAGCGTTAGCGTAATGAGCGACTTCGCCGCCGGCGCAAACCTTGATGACAAACACTACTTTGGCATCAACT
GGGAGCGCGATCTGCCTCTGGCACAAGCAGCCGATATCCGTAACGTGGTTGAAGGCGAGCCAACACCTGATGGTTTAGGT
ACTTACGCGATGGCGCGTGGTATCGAAGTGGGTCATATCTTCCAACTCGGTACTAACTATTCTAAATCGATGAATGCGAC
TGTTCTCGATGAGAACGGTAAATCACAAGTGCTGCTGATGGGTTGTTACGGTGTGGGCGTGAGCCGTATCGTGGCGGCAG
CCATTGAGCAAAACTTCGATGATCGCGGCATTGTATGGCCAGAAGCCATTGCCCCATTCAGTGTAGGCATTCTGCCAATG
AATATGCACAAATCACACCGCGTGACCGATATCGCCGAGCAGTTATACAAAGACTTAAGTGCTGTGGGTATCGATGTGCT
ATTGGATGACCGTAAAGAGCGCCCAGGCGTCATGTTCGCCGATATGGAACTGATTGGTATTCCACACACTGTGGTGATTG
GCGATCGCAATATCGACGCTGGCGTATTTGAATACAAAAACCGCCGCACCGGTGAAAAGCAAGATGTGCCATTCGACCAA
ATCGTCGATTTCTTAAAAAATCTGCAAGCTTAA

Upstream 100 bases:

>100_bases
TTTGTCTCCCAGCTAGTTGAATTTAACCTAAATCCATAGGTGACATCATCAAAGCAGGTGATAAAATGGCGCCCATTCAA
CTCAAATTTGGACATCGGTA

Downstream 100 bases:

>100_bases
GCGAAGGCTTACTGCGCCGAACTTAGTGCGCCGACACAGTCCGTTTGAGCAGATGCTTAAATAAGATTCTCGCGATATAA
ATGCAAACGCACTTGAGTCT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREEMNKAGAIEILMPMVQPADLW
VETGRWDKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRKEVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHLDVDTMNETYEAMYQAYSNILSRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPM
PTEARGAATEALRLVDTPNAKTIAELVEQFGLDITKTVKTLIVKGATEEAPLVALIVRGDHELNEIKADKLDLVASPLEF
APEALIRDAIGAGPGSLGPVGLNMPVIIDHSVSVMSDFAAGANLDDKHYFGINWERDLPLAQAADIRNVVEGEPTPDGLG
TYAMARGIEVGHIFQLGTNYSKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIVWPEAIAPFSVGILPM
NMHKSHRVTDIAEQLYKDLSAVGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDAGVFEYKNRRTGEKQDVPFDQ
IVDFLKNLQA

Sequences:

>Translated_570_residues
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREEMNKAGAIEILMPMVQPADLW
VETGRWDKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRKEVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHLDVDTMNETYEAMYQAYSNILSRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPM
PTEARGAATEALRLVDTPNAKTIAELVEQFGLDITKTVKTLIVKGATEEAPLVALIVRGDHELNEIKADKLDLVASPLEF
APEALIRDAIGAGPGSLGPVGLNMPVIIDHSVSVMSDFAAGANLDDKHYFGINWERDLPLAQAADIRNVVEGEPTPDGLG
TYAMARGIEVGHIFQLGTNYSKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIVWPEAIAPFSVGILPM
NMHKSHRVTDIAEQLYKDLSAVGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDAGVFEYKNRRTGEKQDVPFDQ
IVDFLKNLQA
>Mature_570_residues
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREEMNKAGAIEILMPMVQPADLW
VETGRWDKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRKEVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHLDVDTMNETYEAMYQAYSNILSRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPM
PTEARGAATEALRLVDTPNAKTIAELVEQFGLDITKTVKTLIVKGATEEAPLVALIVRGDHELNEIKADKLDLVASPLEF
APEALIRDAIGAGPGSLGPVGLNMPVIIDHSVSVMSDFAAGANLDDKHYFGINWERDLPLAQAADIRNVVEGEPTPDGLG
TYAMARGIEVGHIFQLGTNYSKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIVWPEAIAPFSVGILPM
NMHKSHRVTDIAEQLYKDLSAVGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDAGVFEYKNRRTGEKQDVPFDQ
IVDFLKNLQA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=214, Percent_Identity=45.3271028037383, Blast_Score=208, Evalue=9e-54,
Organism=Escherichia coli, GI1786392, Length=566, Percent_Identity=71.7314487632509, Blast_Score=844, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=260, Percent_Identity=38.0769230769231, Blast_Score=181, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI193203271, Length=105, Percent_Identity=36.1904761904762, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=540, Percent_Identity=30.1851851851852, Blast_Score=240, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24656200, Length=234, Percent_Identity=38.034188034188, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63138; Mature: 63138

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREE
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGAIEILMPMVQPADLWVETGRWDKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRK
HCCCCCHHHHHHHCCCCHHEEECCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHLDVDTMNETYEAMYQA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
YSNILSRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPM
HHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHCHHHCCCCC
PTEARGAATEALRLVDTPNAKTIAELVEQFGLDITKTVKTLIVKGATEEAPLVALIVRGD
CCCCCCCHHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECC
HELNEIKADKLDLVASPLEFAPEALIRDAIGAGPGSLGPVGLNMPVIIDHSVSVMSDFAA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHC
GANLDDKHYFGINWERDLPLAQAADIRNVVEGEPTPDGLGTYAMARGIEVGHIFQLGTNY
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEEECCCC
SKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIVWPEAIAPFSVGILPM
CCCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEE
NMHKSHRVTDIAEQLYKDLSAVGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDA
CCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEECEEEECCCEEEEEECCCCCC
GVFEYKNRRTGEKQDVPFDQIVDFLKNLQA
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREE
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGAIEILMPMVQPADLWVETGRWDKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRK
HCCCCCHHHHHHHCCCCHHEEECCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHLDVDTMNETYEAMYQA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
YSNILSRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPM
HHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHCHHHCCCCC
PTEARGAATEALRLVDTPNAKTIAELVEQFGLDITKTVKTLIVKGATEEAPLVALIVRGD
CCCCCCCHHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECC
HELNEIKADKLDLVASPLEFAPEALIRDAIGAGPGSLGPVGLNMPVIIDHSVSVMSDFAA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHC
GANLDDKHYFGINWERDLPLAQAADIRNVVEGEPTPDGLGTYAMARGIEVGHIFQLGTNY
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEEECCCC
SKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIVWPEAIAPFSVGILPM
CCCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEE
NMHKSHRVTDIAEQLYKDLSAVGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDA
CCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEECEEEECCCEEEEEECCCCCC
GVFEYKNRRTGEKQDVPFDQIVDFLKNLQA
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA