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The map label for this gene is ynfL [H]

Identifier: 117919726

GI number: 117919726

Start: 1489643

End: 1490584

Strand: Direct

Name: ynfL [H]

Synonym: Shewana3_1278

Alternate gene names: 117919726

Gene position: 1489643-1490584 (Clockwise)

Preceding gene: 117919725

Following gene: 117919727

Centisome position: 29.96

GC content: 59.55

Gene sequence:

>942_bases
ATGGAACTTCGCCACCTTCGTTGCTTCCTTGCCGTTGCTGACGAACTCCACTTCGCTCGTGCGGCGGAGAAACTGCACAT
CGAACAGTCACCGCTGTCACGTGCTATCAAAGAACTGGAAGAAGACTTGGGCGAGCAGTTATTCGTCCGCACCAGCCGCA
GCACACGGTTGACACGGGCAGGCAAACTGTTTTTGGAGCATGTGCCGCGCGTCTTCACTGCTTTGGAGCAGGCGCGCGAC
AGCGTGAAGGCTGCAGCCAATGGCTTTCACGGACAGCTGCGCATCGCGTTGTCCGACGGCATCACACCATCGCGTCTACC
GATCTTGCTGGCGCTTTGCAGACAGGAGGAACCGGAAGTTGAACTCAGATTCTTCGAGGTGCCGCTGTCGCAGCAGATCA
AGGGGTTGCAGGACGACCTGTATGACGTGGGATTCGCACAATCCGATGACGTGGGTGACGGCATCACCGTAGAGGCGGTG
TGGCACGATCCGCTCATGGTCGCAGTACCAGCCCGGCATCCATTGCTGATACATAAACGCATTCCTCTCGATGAAGTGCT
GCGCTATCCACTGGTGCTATGTGATCCCAAGGTATGTGAGGGCCATACACGCCAGGTCGAACGTGTGTTGCGGCGTGCGG
AGCAAGAACCCTTGATCGCCGAGCGCGTGGCCTCCTGCGACCTGATGATGGCGCTGGTGTCAGCCGGCTTTGCTCTGGGC
CTGGCGGGCGCGCCGCATATCGCTTCCAGCCGCGAATCTGGTGTGGTCGCTCGGCCACTAGCGGGCCGTTCGCCGATGCT
GACAACGTATCTGCTGCATCGGGAAGCGGAGACCTCGGAAGTGCTGGCCCGTTTCATCGAGCGGGTACAGGCTATCGAAT
CGCCTGAGGGCGTCAGGCTAGTGCCACCGCCCGAATCCGATCAGGAGGAAATCGAACTATGA

Upstream 100 bases:

>100_bases
AACGACGCCGCATTACTTGGAAGGCGTGGCGCGTGTGCAAGCATGTTGCGAGTCCGTGGAACTTCAGCGGATCTCCTGGT
TTCAGGAATGAGGATACGGC

Downstream 100 bases:

>100_bases
AGCAGATCGTCCCATTATTACTGGTCGCGACATTGGCCGGATGCGGTCAGTCGGACTCGCCTCGACAGGCCGACGTGTTG
ACTGTGGAAGAACTGGCTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MELRHLRCFLAVADELHFARAAEKLHIEQSPLSRAIKELEEDLGEQLFVRTSRSTRLTRAGKLFLEHVPRVFTALEQARD
SVKAAANGFHGQLRIALSDGITPSRLPILLALCRQEEPEVELRFFEVPLSQQIKGLQDDLYDVGFAQSDDVGDGITVEAV
WHDPLMVAVPARHPLLIHKRIPLDEVLRYPLVLCDPKVCEGHTRQVERVLRRAEQEPLIAERVASCDLMMALVSAGFALG
LAGAPHIASSRESGVVARPLAGRSPMLTTYLLHREAETSEVLARFIERVQAIESPEGVRLVPPPESDQEEIEL

Sequences:

>Translated_313_residues
MELRHLRCFLAVADELHFARAAEKLHIEQSPLSRAIKELEEDLGEQLFVRTSRSTRLTRAGKLFLEHVPRVFTALEQARD
SVKAAANGFHGQLRIALSDGITPSRLPILLALCRQEEPEVELRFFEVPLSQQIKGLQDDLYDVGFAQSDDVGDGITVEAV
WHDPLMVAVPARHPLLIHKRIPLDEVLRYPLVLCDPKVCEGHTRQVERVLRRAEQEPLIAERVASCDLMMALVSAGFALG
LAGAPHIASSRESGVVARPLAGRSPMLTTYLLHREAETSEVLARFIERVQAIESPEGVRLVPPPESDQEEIEL
>Mature_313_residues
MELRHLRCFLAVADELHFARAAEKLHIEQSPLSRAIKELEEDLGEQLFVRTSRSTRLTRAGKLFLEHVPRVFTALEQARD
SVKAAANGFHGQLRIALSDGITPSRLPILLALCRQEEPEVELRFFEVPLSQQIKGLQDDLYDVGFAQSDDVGDGITVEAV
WHDPLMVAVPARHPLLIHKRIPLDEVLRYPLVLCDPKVCEGHTRQVERVLRRAEQEPLIAERVASCDLMMALVSAGFALG
LAGAPHIASSRESGVVARPLAGRSPMLTTYLLHREAETSEVLARFIERVQAIESPEGVRLVPPPESDQEEIEL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=242, Percent_Identity=34.7107438016529, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=32.2033898305085, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI2367136, Length=287, Percent_Identity=30.6620209059233, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI157672245, Length=231, Percent_Identity=29.8701298701299, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=26.6393442622951, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=29.7435897435897, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=24.5791245791246, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787601, Length=279, Percent_Identity=24.3727598566308, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI145693105, Length=262, Percent_Identity=24.4274809160305, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788297, Length=127, Percent_Identity=33.8582677165354, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788748, Length=241, Percent_Identity=24.0663900414938, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787806, Length=301, Percent_Identity=23.9202657807309, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786713, Length=143, Percent_Identity=33.5664335664336, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787949, Length=307, Percent_Identity=25.7328990228013, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=29.1338582677165, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082132, Length=144, Percent_Identity=30.5555555555556, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788296, Length=311, Percent_Identity=26.0450160771704, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34923; Mature: 34923

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRCFLAVADELHFARAAEKLHIEQSPLSRAIKELEEDLGEQLFVRTSRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHH
GKLFLEHVPRVFTALEQARDSVKAAANGFHGQLRIALSDGITPSRLPILLALCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCE
ELRFFEVPLSQQIKGLQDDLYDVGFAQSDDVGDGITVEAVWHDPLMVAVPARHPLLIHKR
EEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCEEEECC
IPLDEVLRYPLVLCDPKVCEGHTRQVERVLRRAEQEPLIAERVASCDLMMALVSAGFALG
CCHHHHHHCCEEEECCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHH
LAGAPHIASSRESGVVARPLAGRSPMLTTYLLHREAETSEVLARFIERVQAIESPEGVRL
CCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEE
VPPPESDQEEIEL
CCCCCCCHHHCCC
>Mature Secondary Structure
MELRHLRCFLAVADELHFARAAEKLHIEQSPLSRAIKELEEDLGEQLFVRTSRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHH
GKLFLEHVPRVFTALEQARDSVKAAANGFHGQLRIALSDGITPSRLPILLALCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCE
ELRFFEVPLSQQIKGLQDDLYDVGFAQSDDVGDGITVEAVWHDPLMVAVPARHPLLIHKR
EEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCEEEECC
IPLDEVLRYPLVLCDPKVCEGHTRQVERVLRRAEQEPLIAERVASCDLMMALVSAGFALG
CCHHHHHHCCEEEECCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHH
LAGAPHIASSRESGVVARPLAGRSPMLTTYLLHREAETSEVLARFIERVQAIESPEGVRL
CCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEE
VPPPESDQEEIEL
CCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]