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The map label for this gene is actII-2 [H]

Identifier: 117919718

GI number: 117919718

Start: 1483912

End: 1485396

Strand: Direct

Name: actII-2 [H]

Synonym: Shewana3_1270

Alternate gene names: 117919718

Gene position: 1483912-1485396 (Clockwise)

Preceding gene: 117919716

Following gene: 117919719

Centisome position: 29.84

GC content: 56.36

Gene sequence:

>1485_bases
ATGATCGCTTTATCGCGATGGAGATGCAATATGAACGATGATTCTCATCTTTTCACAACCTGTACCGCCCCTGCCAGACG
TCCCCCATGGCTCGGGTTGTCCGTCCTGTTGCTGGCTGGCTTCGTCACCATCTTTGACCTGTTTGTGGTCAATGTGGCCA
TCCCCAGCATGCAAGCAAACTTGGGGGCGAGCTTTGCGCAGATTGGCTTTATCGTCGCGGGTTATGAACTGGCCTTCGGT
GTATTGCTTATTATGGGCGGACGATTAGGCGATTTGTTTGGTCGCCGCCGGTTATTCATCGTTGGTATGGCGGGATTCAC
GTTGGCATCGGCGATGTGTGGGTTAGCCCCCAGCGCAGAAATTTTGATCGTTGCGCGCGTGTTGCAGGGCCTAGCTGCTG
CATTACTGTTCCCGCAGGTCTATGCTTCCATTCGCGTGAATTTCGACGGCGACGATAGTCGGCGCGCCTTTGGCTTGCTG
GGTATGACGCTGGGCCTGGCCGCCATCGCTGGCCAGGTGCTGGGTGGTTGGCTGGTGCATGCCGATCTGTTCGGATTAAG
CTGGCGTACGATCTTCCTCATCAACGTACCTATCGGGCTGTTCGCGATCACCGCAGCATCTTGCATTCCCGAGTCCAGAG
CCGAGCAAAGCCCGGCACTGGATTGGAGTGGTGTGATACTGGTCAGTAGTGGGCTGGCACTCTTGCTGGTGCCGCTGATT
GAGGGGCCTGGGCAAGGCTGGCCTGCCTGGAGCCTGTGGAGCTTAGGCGGCGCCGTAGCGCTGATGGTGACGTTTTATCG
GTATCAGGAGCGTCAGCGAATCGCCGGACGTTTTCCGTTAGTGGATATGCAACTGATGAGGCAGCGTCGCTTTGCGCTGG
GAGCCTTGTTGGTTCTGCTAGTCTATTCCACATCCAGCTCATTCTTTTTGTCCTTTGCCTTGCTGGTGCAAACCGGTTTG
GGGCTTGACCCCTTCGTCGCGGGCAGCATCTTCGCACCATGCAGCGTGGGCTTCGTCTTGGCATCACTAACCGCACCCCG
GCTAGTGGCACTATGGGGAGCGCGCGCTATCGTCGCAGGCGCGTTGGTCTACGCGATTTCCATCGGGCTGCTGATTACGC
AAGTGCAGATGGCTGGCGCAGAGCTGCTGCCGGTAACGTTGATTCCCGTGTTAATCATAGTCGGAGCTGGTCAGGGTTTA
ATAATGACGCCACTACTGAACCTGGTACTGGGCTTTGTAAAGGAGAACCAAGCTGGTATGGCGTCTGGTGTGATTTCCAC
TGTTCAGCAGGTCGGTGCTGCATTGGGTGTAGCCATAGTCGGCATCCTGTTCGGGACGGCGTTGACCGCAAGTAACGGTG
CGTTAGCGCAAGCGGACCAATACGCTTCGGCCTTTGTCTCGGGAATGCTCTACAACTTGGGTGCCGCGCTACTTATATGC
TTTTTGTTGCTTATTCTGGCAAGGTCGCAAAGGTCGGCTAGCTAG

Upstream 100 bases:

>100_bases
AGTTCGTCGCGAGTTCGTTTCAAGGTGCGCATAGTTTATCTCGTTGCTTTTAGTAATAGCATAAGTTGTTAAATTGTACT
TGTTTAAAAAGGGTTTGAGA

Downstream 100 bases:

>100_bases
CAAAAGAGGAGTTAATGGAATGTCGGCATCTGCGTTGCCTTCTGGCCGTGGCCGAAGAACTACACTTCGCTCGTGCTGCT
GAACGACTGCATATTGAGCA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MIALSRWRCNMNDDSHLFTTCTAPARRPPWLGLSVLLLAGFVTIFDLFVVNVAIPSMQANLGASFAQIGFIVAGYELAFG
VLLIMGGRLGDLFGRRRLFIVGMAGFTLASAMCGLAPSAEILIVARVLQGLAAALLFPQVYASIRVNFDGDDSRRAFGLL
GMTLGLAAIAGQVLGGWLVHADLFGLSWRTIFLINVPIGLFAITAASCIPESRAEQSPALDWSGVILVSSGLALLLVPLI
EGPGQGWPAWSLWSLGGAVALMVTFYRYQERQRIAGRFPLVDMQLMRQRRFALGALLVLLVYSTSSSFFLSFALLVQTGL
GLDPFVAGSIFAPCSVGFVLASLTAPRLVALWGARAIVAGALVYAISIGLLITQVQMAGAELLPVTLIPVLIIVGAGQGL
IMTPLLNLVLGFVKENQAGMASGVISTVQQVGAALGVAIVGILFGTALTASNGALAQADQYASAFVSGMLYNLGAALLIC
FLLLILARSQRSAS

Sequences:

>Translated_494_residues
MIALSRWRCNMNDDSHLFTTCTAPARRPPWLGLSVLLLAGFVTIFDLFVVNVAIPSMQANLGASFAQIGFIVAGYELAFG
VLLIMGGRLGDLFGRRRLFIVGMAGFTLASAMCGLAPSAEILIVARVLQGLAAALLFPQVYASIRVNFDGDDSRRAFGLL
GMTLGLAAIAGQVLGGWLVHADLFGLSWRTIFLINVPIGLFAITAASCIPESRAEQSPALDWSGVILVSSGLALLLVPLI
EGPGQGWPAWSLWSLGGAVALMVTFYRYQERQRIAGRFPLVDMQLMRQRRFALGALLVLLVYSTSSSFFLSFALLVQTGL
GLDPFVAGSIFAPCSVGFVLASLTAPRLVALWGARAIVAGALVYAISIGLLITQVQMAGAELLPVTLIPVLIIVGAGQGL
IMTPLLNLVLGFVKENQAGMASGVISTVQQVGAALGVAIVGILFGTALTASNGALAQADQYASAFVSGMLYNLGAALLIC
FLLLILARSQRSAS
>Mature_494_residues
MIALSRWRCNMNDDSHLFTTCTAPARRPPWLGLSVLLLAGFVTIFDLFVVNVAIPSMQANLGASFAQIGFIVAGYELAFG
VLLIMGGRLGDLFGRRRLFIVGMAGFTLASAMCGLAPSAEILIVARVLQGLAAALLFPQVYASIRVNFDGDDSRRAFGLL
GMTLGLAAIAGQVLGGWLVHADLFGLSWRTIFLINVPIGLFAITAASCIPESRAEQSPALDWSGVILVSSGLALLLVPLI
EGPGQGWPAWSLWSLGGAVALMVTFYRYQERQRIAGRFPLVDMQLMRQRRFALGALLVLLVYSTSSSFFLSFALLVQTGL
GLDPFVAGSIFAPCSVGFVLASLTAPRLVALWGARAIVAGALVYAISIGLLITQVQMAGAELLPVTLIPVLIIVGAGQGL
IMTPLLNLVLGFVKENQAGMASGVISTVQQVGAALGVAIVGILFGTALTASNGALAQADQYASAFVSGMLYNLGAALLIC
FLLLILARSQRSAS

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=26.8115942028986, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87081983, Length=409, Percent_Identity=26.4058679706601, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788392, Length=410, Percent_Identity=26.5853658536585, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788710, Length=447, Percent_Identity=25.2796420581655, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789042, Length=270, Percent_Identity=29.2592592592593, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790146, Length=147, Percent_Identity=29.9319727891156, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51952; Mature: 51952

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALSRWRCNMNDDSHLFTTCTAPARRPPWLGLSVLLLAGFVTIFDLFVVNVAIPSMQAN
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LGASFAQIGFIVAGYELAFGVLLIMGGRLGDLFGRRRLFIVGMAGFTLASAMCGLAPSAE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEHHHHHHHHHHCCCCCHH
ILIVARVLQGLAAALLFPQVYASIRVNFDGDDSRRAFGLLGMTLGLAAIAGQVLGGWLVH
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLFGLSWRTIFLINVPIGLFAITAASCIPESRAEQSPALDWSGVILVSSGLALLLVPLI
HHHHCCCEEEEEEEECCHHHHHHHHHHHCCHHHCCCCCCCCCCCHHEECCCHHHHHHHHH
EGPGQGWPAWSLWSLGGAVALMVTFYRYQERQRIAGRFPLVDMQLMRQRRFALGALLVLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VYSTSSSFFLSFALLVQTGLGLDPFVAGSIFAPCSVGFVLASLTAPRLVALWGARAIVAG
HHCCCCHHHHHHHHHHHHCCCCCCHHHCCHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALVYAISIGLLITQVQMAGAELLPVTLIPVLIIVGAGQGLIMTPLLNLVLGFVKENQAGM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHH
ASGVISTVQQVGAALGVAIVGILFGTALTASNGALAQADQYASAFVSGMLYNLGAALLIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLLILARSQRSAS
HHHHHHHHCCCCCC
>Mature Secondary Structure
MIALSRWRCNMNDDSHLFTTCTAPARRPPWLGLSVLLLAGFVTIFDLFVVNVAIPSMQAN
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LGASFAQIGFIVAGYELAFGVLLIMGGRLGDLFGRRRLFIVGMAGFTLASAMCGLAPSAE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEHHHHHHHHHHCCCCCHH
ILIVARVLQGLAAALLFPQVYASIRVNFDGDDSRRAFGLLGMTLGLAAIAGQVLGGWLVH
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLFGLSWRTIFLINVPIGLFAITAASCIPESRAEQSPALDWSGVILVSSGLALLLVPLI
HHHHCCCEEEEEEEECCHHHHHHHHHHHCCHHHCCCCCCCCCCCHHEECCCHHHHHHHHH
EGPGQGWPAWSLWSLGGAVALMVTFYRYQERQRIAGRFPLVDMQLMRQRRFALGALLVLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VYSTSSSFFLSFALLVQTGLGLDPFVAGSIFAPCSVGFVLASLTAPRLVALWGARAIVAG
HHCCCCHHHHHHHHHHHHCCCCCCHHHCCHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALVYAISIGLLITQVQMAGAELLPVTLIPVLIIVGAGQGLIMTPLLNLVLGFVKENQAGM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHH
ASGVISTVQQVGAALGVAIVGILFGTALTASNGALAQADQYASAFVSGMLYNLGAALLIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLLILARSQRSAS
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]