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The map label for this gene is ycdT [H]

Identifier: 117919199

GI number: 117919199

Start: 866449

End: 868389

Strand: Reverse

Name: ycdT [H]

Synonym: Shewana3_0747

Alternate gene names: 117919199

Gene position: 868389-866449 (Counterclockwise)

Preceding gene: 117919202

Following gene: 117919197

Centisome position: 17.46

GC content: 45.29

Gene sequence:

>1941_bases
ATGATTAGCTATCTGTTTCAGTGCGAACAGGAAACCTTTCCCTTGCCACACACTCAGCGTAATTTATCTCTGTTTCTGAT
GCTGCTGTTAGTGCCGCTAAGCCTTGCCGTTGCAGAAGAAAAGCCACAGATCGATCAAATATTCAAATTATTTGAAAGTG
GTGAGGTTATGGCTCGCGAGTCGGTAGAGCAAAATATCGCGCTACTGGATAAACTGATTGCCAGTGATGATGTAGAACAC
ATCGAAAAGTTAAAAATGCTCAAATGCTGGAATCAACCAGCCGAGACAGAAGCTGAAATCCTTCTCGCCCGAGACAAAGC
ACAAGAAGCGCTGAATAGCATTCCCACCACAGCCTCAGTCCATTTTGCCACCGATCTAACCCTATGCCACTCCTGGTTTC
TACAATTAAGTGGAGATATTAATGGCGCTATGGAAGGGTATTCTCGCAGCATTAAAACTGCATACGAACATGAAGATATC
AAGCTTATCGCCGATGCCCGCAGCTTACGTGGCGCACTCTACTCCTATATTGGTGACTTTTCCGCCGCCTTAGACGATTT
AGTCACCGCACAAGGGCTGTACGAATCCTTAAATTTAACTGGCTGGGCAAACATCAATCTAACCGACATTGCCACCAGCT
TTAGGCGCTATGGTGATCCCGAGAGTGCAATCAAGTATTACAACAAACTCAAAGAGTTATATCTAAAGAAAGGGAACGAG
GAGCAAGTCGCTAATGTCACCTCCGATATAGGGATTGCCTTAGATGAAATGGGCGAGCACGAACAGGCCATTGAAAACTT
TCGTTTTAGCTATCAGTACTTTAAAAAGCATCGACTCAACAATGCCGCCGCGGTCAACGCCACTAACATCGCCTACAGCT
TGATCCAACTAAATCGTTTAGATGAGGCCGAAAAATATCTAAAAGAAGCCGAGTTAGAGATCACGGCGAACGAACCCGCA
GCCTACAGCTTTATGAAGTTGTTTATGGGCAAAATTCGTTATCAACAAGGTCGCTATCAAGAGGCGTTAAGCGAACTCGC
CGACGCTGAAAATGCGTTTCGCGCCATGCAGAATGATCGCGGCTTAGTTCAGCTGTTGCAACTCAAGAGCGAAGTCTATG
CGGCGATGAATGATTTGCCGTCGGCCTATCAAACTTTACAGCAGTTTGTCGCCTTGACTCGAAAGGTGGAAGACAATTCG
TTTGCGCACCAAACCACTGAGTTAAAAGTAAAATTTGATACCAGTTGGATTGAGTCAGAAAACAAACGCTTACTCGATAA
CCAAAAATTAAAGGAACAAGAGTTAGCACTGCTCGAGAAAAATAAGTCGCTGCAACTTATCATCCTACTACTCACGGGCT
GCATTTTGGCCATAGTGTCACTCTTTGCCTACAAACAAGTGCACCGCAATCGTCACCTACAGACCATAGCCCTAACCGAC
TATCTGACCCAATTGCCCAATCGTCGATACATCTATGCCAAGGCGGAACAATATTTTCAACAGGCGTTAAAGCAACAAAC
CCCGTTATCGGTAATCATCTTCGATGCGGATCATTTTAAAAAGATTAACGATAATTTCGGCCATGAAATTGGCGACCAAG
CACTATTAACCATAGCCCAAGCCAGCCAGGCATTAACTGCTGACCGCAACTTGATTGGCCGTATCGGTGGCGAAGAGTTT
TTAATTTTATTACCCAATACCAACGCCGAACGCGTGTGGGAACTCGCCCATGAGCTGCAAACCTTAATCACCCGTTATGG
CGCCATTAATTTACCCGTAGAGTTAAAGCTAACTGTAAGTGCAGGGGTGGCAACTTTAAATACCGAATTAGGCAAACAAG
ATGACAGCTTTGCGCGTTTGCTTAAGCGTGCGGACAACGCCCTTTACGACGCTAAAAATGCTGGTAAAAACTGCGTCAAA
GTCGCAAAAGCCACGAGCTAA

Upstream 100 bases:

>100_bases
GGGTGAGATCCAAGGCGACGTTTATACCTTGATAGTGGAATGAATGCAAAAATTGCAAACTTTACATGTAAACAAGACGT
AAAAAGTTTATGCTAAGATA

Downstream 100 bases:

>100_bases
CTTTTAGGTATTGCGATGCCAAAAGATTTGATGTTGAAGAGGTGGCGGTAAGGCGCCAAACCATTTTTGATAGAGCCGTG
CAAAATCTCCTGATTCTTTA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MISYLFQCEQETFPLPHTQRNLSLFLMLLLVPLSLAVAEEKPQIDQIFKLFESGEVMARESVEQNIALLDKLIASDDVEH
IEKLKMLKCWNQPAETEAEILLARDKAQEALNSIPTTASVHFATDLTLCHSWFLQLSGDINGAMEGYSRSIKTAYEHEDI
KLIADARSLRGALYSYIGDFSAALDDLVTAQGLYESLNLTGWANINLTDIATSFRRYGDPESAIKYYNKLKELYLKKGNE
EQVANVTSDIGIALDEMGEHEQAIENFRFSYQYFKKHRLNNAAAVNATNIAYSLIQLNRLDEAEKYLKEAELEITANEPA
AYSFMKLFMGKIRYQQGRYQEALSELADAENAFRAMQNDRGLVQLLQLKSEVYAAMNDLPSAYQTLQQFVALTRKVEDNS
FAHQTTELKVKFDTSWIESENKRLLDNQKLKEQELALLEKNKSLQLIILLLTGCILAIVSLFAYKQVHRNRHLQTIALTD
YLTQLPNRRYIYAKAEQYFQQALKQQTPLSVIIFDADHFKKINDNFGHEIGDQALLTIAQASQALTADRNLIGRIGGEEF
LILLPNTNAERVWELAHELQTLITRYGAINLPVELKLTVSAGVATLNTELGKQDDSFARLLKRADNALYDAKNAGKNCVK
VAKATS

Sequences:

>Translated_646_residues
MISYLFQCEQETFPLPHTQRNLSLFLMLLLVPLSLAVAEEKPQIDQIFKLFESGEVMARESVEQNIALLDKLIASDDVEH
IEKLKMLKCWNQPAETEAEILLARDKAQEALNSIPTTASVHFATDLTLCHSWFLQLSGDINGAMEGYSRSIKTAYEHEDI
KLIADARSLRGALYSYIGDFSAALDDLVTAQGLYESLNLTGWANINLTDIATSFRRYGDPESAIKYYNKLKELYLKKGNE
EQVANVTSDIGIALDEMGEHEQAIENFRFSYQYFKKHRLNNAAAVNATNIAYSLIQLNRLDEAEKYLKEAELEITANEPA
AYSFMKLFMGKIRYQQGRYQEALSELADAENAFRAMQNDRGLVQLLQLKSEVYAAMNDLPSAYQTLQQFVALTRKVEDNS
FAHQTTELKVKFDTSWIESENKRLLDNQKLKEQELALLEKNKSLQLIILLLTGCILAIVSLFAYKQVHRNRHLQTIALTD
YLTQLPNRRYIYAKAEQYFQQALKQQTPLSVIIFDADHFKKINDNFGHEIGDQALLTIAQASQALTADRNLIGRIGGEEF
LILLPNTNAERVWELAHELQTLITRYGAINLPVELKLTVSAGVATLNTELGKQDDSFARLLKRADNALYDAKNAGKNCVK
VAKATS
>Mature_646_residues
MISYLFQCEQETFPLPHTQRNLSLFLMLLLVPLSLAVAEEKPQIDQIFKLFESGEVMARESVEQNIALLDKLIASDDVEH
IEKLKMLKCWNQPAETEAEILLARDKAQEALNSIPTTASVHFATDLTLCHSWFLQLSGDINGAMEGYSRSIKTAYEHEDI
KLIADARSLRGALYSYIGDFSAALDDLVTAQGLYESLNLTGWANINLTDIATSFRRYGDPESAIKYYNKLKELYLKKGNE
EQVANVTSDIGIALDEMGEHEQAIENFRFSYQYFKKHRLNNAAAVNATNIAYSLIQLNRLDEAEKYLKEAELEITANEPA
AYSFMKLFMGKIRYQQGRYQEALSELADAENAFRAMQNDRGLVQLLQLKSEVYAAMNDLPSAYQTLQQFVALTRKVEDNS
FAHQTTELKVKFDTSWIESENKRLLDNQKLKEQELALLEKNKSLQLIILLLTGCILAIVSLFAYKQVHRNRHLQTIALTD
YLTQLPNRRYIYAKAEQYFQQALKQQTPLSVIIFDADHFKKINDNFGHEIGDQALLTIAQASQALTADRNLIGRIGGEEF
LILLPNTNAERVWELAHELQTLITRYGAINLPVELKLTVSAGVATLNTELGKQDDSFARLLKRADNALYDAKNAGKNCVK
VAKATS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=193, Percent_Identity=36.2694300518135, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786584, Length=200, Percent_Identity=32, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1787802, Length=176, Percent_Identity=35.2272727272727, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=36.4705882352941, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI145693134, Length=210, Percent_Identity=32.8571428571429, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082007, Length=209, Percent_Identity=33.4928229665072, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787816, Length=178, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=33.7278106508876, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=32.7380952380952, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=28.6549707602339, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788085, Length=193, Percent_Identity=25.9067357512953, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 73124; Mature: 73124

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50293 TPR_REGION ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISYLFQCEQETFPLPHTQRNLSLFLMLLLVPLSLAVAEEKPQIDQIFKLFESGEVMARE
CCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHH
SVEQNIALLDKLIASDDVEHIEKLKMLKCWNQPAETEAEILLARDKAQEALNSIPTTASV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
HFATDLTLCHSWFLQLSGDINGAMEGYSRSIKTAYEHEDIKLIADARSLRGALYSYIGDF
EHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHH
SAALDDLVTAQGLYESLNLTGWANINLTDIATSFRRYGDPESAIKYYNKLKELYLKKGNE
HHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCH
EQVANVTSDIGIALDEMGEHEQAIENFRFSYQYFKKHRLNNAAAVNATNIAYSLIQLNRL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHH
DEAEKYLKEAELEITANEPAAYSFMKLFMGKIRYQQGRYQEALSELADAENAFRAMQNDR
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
GLVQLLQLKSEVYAAMNDLPSAYQTLQQFVALTRKVEDNSFAHQTTELKVKFDTSWIESE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHCCC
NKRLLDNQKLKEQELALLEKNKSLQLIILLLTGCILAIVSLFAYKQVHRNRHLQTIALTD
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
YLTQLPNRRYIYAKAEQYFQQALKQQTPLSVIIFDADHFKKINDNFGHEIGDQALLTIAQ
HHHHCCCCEEEEEHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCHHHCHHHHHHHHH
ASQALTADRNLIGRIGGEEFLILLPNTNAERVWELAHELQTLITRYGAINLPVELKLTVS
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
AGVATLNTELGKQDDSFARLLKRADNALYDAKNAGKNCVKVAKATS
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MISYLFQCEQETFPLPHTQRNLSLFLMLLLVPLSLAVAEEKPQIDQIFKLFESGEVMARE
CCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHH
SVEQNIALLDKLIASDDVEHIEKLKMLKCWNQPAETEAEILLARDKAQEALNSIPTTASV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
HFATDLTLCHSWFLQLSGDINGAMEGYSRSIKTAYEHEDIKLIADARSLRGALYSYIGDF
EHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHH
SAALDDLVTAQGLYESLNLTGWANINLTDIATSFRRYGDPESAIKYYNKLKELYLKKGNE
HHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCH
EQVANVTSDIGIALDEMGEHEQAIENFRFSYQYFKKHRLNNAAAVNATNIAYSLIQLNRL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHH
DEAEKYLKEAELEITANEPAAYSFMKLFMGKIRYQQGRYQEALSELADAENAFRAMQNDR
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
GLVQLLQLKSEVYAAMNDLPSAYQTLQQFVALTRKVEDNSFAHQTTELKVKFDTSWIESE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHCCC
NKRLLDNQKLKEQELALLEKNKSLQLIILLLTGCILAIVSLFAYKQVHRNRHLQTIALTD
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
YLTQLPNRRYIYAKAEQYFQQALKQQTPLSVIIFDADHFKKINDNFGHEIGDQALLTIAQ
HHHHCCCCEEEEEHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCHHHCHHHHHHHHH
ASQALTADRNLIGRIGGEEFLILLPNTNAERVWELAHELQTLITRYGAINLPVELKLTVS
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
AGVATLNTELGKQDDSFARLLKRADNALYDAKNAGKNCVKVAKATS
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]