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The map label for this gene is thiH [H]

Identifier: 117919160

GI number: 117919160

Start: 822821

End: 824260

Strand: Reverse

Name: thiH [H]

Synonym: Shewana3_0708

Alternate gene names: 117919160

Gene position: 824260-822821 (Counterclockwise)

Preceding gene: 117919161

Following gene: 117919159

Centisome position: 16.58

GC content: 49.38

Gene sequence:

>1440_bases
ATGAGCACACACGAGCATCATTCCATTACCGTCTCTGACTATAATCCCAACGTCAGCTTTATTGACGATCTGGCGATTTG
GCAGGCCATTGAAGAGGCCAGCAATCCGAGTCGTGAACAAATCCAAGCCATTCTCGAAAAGGCGCGCCAATGCGAAGGCT
TAAGCATTCGCGAAACCGCTCTCCTGCTACAAAATCAAGATAAAGCGCTGGATGAAGCACTCTTTGCCGTCGCCCGTGAG
ATTAAAAACACCATCTACGGCAATCGTATAGTGATGTTTGCGCCACTCTATGTCTCCAACCATTGTGCCAACAGTTGTAG
TTACTGCGGCTTTAATGCCGATAACCATGAGCTGAAACGCAAGACCTTAAAACAGGATGAGATCCGCCAAGAGGTCACCA
TCCTCGAAGAAATGGGCCACAAACGGATCTTGGCCGTTTATGGCGAGCATCCACGCAACAATGTGCAAGCCATTATTGAA
AGTATTCAAACCATGTACAGCGTGAAGCAGGGCAAGGGCGGCGAAATTCGCCGTATCAACGTCAACTGTGCGCCAATGAG
TGTGGAGGACTTTAAACAGCTCAAAACGGCGGCGATAGGCACTTACCAATGCTTCCAAGAAACCTATCATCAAGACACTT
ACAGTGAAGTGCACCTAAAAGGTAAAAAAACTGACTTTTTATACCGCCTCTACGCCATGCACAGAGCCATGGAAGCGGGA
ATCGACGATGTCGGTATCGGCGCCCTCTTTGGCCTGTATGACCATAGATTTGAGCTGCTCGCTATGCTCACTCATGTTCA
ACAACTCGAAAAAGACTGTGGCGTTGGCCCGCATACTATCTCCTTTCCGCGGATAGAACCCGCCCATGGCTCTGCCCTTA
GTGAAAAGCCGCCCTATGAGGTTGATGATGAGTGCTTTAAGCGTATCGTTGCCATCACTCGCCTCGCCGTACCTTATACA
GGCTTGATTATGAGCACGCGGGAGAGCGCCGCAATGCGCAAAGAATTGTTAGAGCTTGGCGTTTCACAGATCAGTGCAGG
CTCACGCACTGCACCCGGTGGTTATCAAGACAGCAAACAAAATCAACACGATGCCGAACAATTTAGCCTTGGCGATCATC
GCGCCATGGATGAAATCATCTATGAATTAGTCACAGATTCGGATGCCATCCCCTCCTTCTGTACGGGCTGTTACCGTAAA
GGGCGCACAGGCGACCACTTTATGGGATTAGCCAAGCAGCAGTTTATTGGCAAGTTCTGCCAGCCCAATGCCTTAATCAC
CTTTAGGGAATATCTGAACGACTACGCCAGCGATAAAACCCGTGAAGCAGGTAACGCCCTGATAGAGCGAGAACTCGCCA
AAATGAGTCCATCACGGGAACGTAATGTGCGCGTTTGCCTGAAAAAAACCGATGCGGGTGAACGGGATATCTATTTGTAA

Upstream 100 bases:

>100_bases
ACGAACGGCGAACAACTGACCAGCAAATAGCTAAGTCATCTGCGGCAGTTGCTCAACAACTGCCGCATTTCTGCATTCAT
ATTCAAGGGCATAGATAACC

Downstream 100 bases:

>100_bases
ATGGCATCTTATTTATTTTCTGAGCCCGGCGTGAAGTTGCATTTTGGCCTTAACACTAGCGCCTTACTGAATGCGATAGA
GCACGCTAATGTAATCGTGT

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSTHEHHSITVSDYNPNVSFIDDLAIWQAIEEASNPSREQIQAILEKARQCEGLSIRETALLLQNQDKALDEALFAVARE
IKNTIYGNRIVMFAPLYVSNHCANSCSYCGFNADNHELKRKTLKQDEIRQEVTILEEMGHKRILAVYGEHPRNNVQAIIE
SIQTMYSVKQGKGGEIRRINVNCAPMSVEDFKQLKTAAIGTYQCFQETYHQDTYSEVHLKGKKTDFLYRLYAMHRAMEAG
IDDVGIGALFGLYDHRFELLAMLTHVQQLEKDCGVGPHTISFPRIEPAHGSALSEKPPYEVDDECFKRIVAITRLAVPYT
GLIMSTRESAAMRKELLELGVSQISAGSRTAPGGYQDSKQNQHDAEQFSLGDHRAMDEIIYELVTDSDAIPSFCTGCYRK
GRTGDHFMGLAKQQFIGKFCQPNALITFREYLNDYASDKTREAGNALIERELAKMSPSRERNVRVCLKKTDAGERDIYL

Sequences:

>Translated_479_residues
MSTHEHHSITVSDYNPNVSFIDDLAIWQAIEEASNPSREQIQAILEKARQCEGLSIRETALLLQNQDKALDEALFAVARE
IKNTIYGNRIVMFAPLYVSNHCANSCSYCGFNADNHELKRKTLKQDEIRQEVTILEEMGHKRILAVYGEHPRNNVQAIIE
SIQTMYSVKQGKGGEIRRINVNCAPMSVEDFKQLKTAAIGTYQCFQETYHQDTYSEVHLKGKKTDFLYRLYAMHRAMEAG
IDDVGIGALFGLYDHRFELLAMLTHVQQLEKDCGVGPHTISFPRIEPAHGSALSEKPPYEVDDECFKRIVAITRLAVPYT
GLIMSTRESAAMRKELLELGVSQISAGSRTAPGGYQDSKQNQHDAEQFSLGDHRAMDEIIYELVTDSDAIPSFCTGCYRK
GRTGDHFMGLAKQQFIGKFCQPNALITFREYLNDYASDKTREAGNALIERELAKMSPSRERNVRVCLKKTDAGERDIYL
>Mature_478_residues
STHEHHSITVSDYNPNVSFIDDLAIWQAIEEASNPSREQIQAILEKARQCEGLSIRETALLLQNQDKALDEALFAVAREI
KNTIYGNRIVMFAPLYVSNHCANSCSYCGFNADNHELKRKTLKQDEIRQEVTILEEMGHKRILAVYGEHPRNNVQAIIES
IQTMYSVKQGKGGEIRRINVNCAPMSVEDFKQLKTAAIGTYQCFQETYHQDTYSEVHLKGKKTDFLYRLYAMHRAMEAGI
DDVGIGALFGLYDHRFELLAMLTHVQQLEKDCGVGPHTISFPRIEPAHGSALSEKPPYEVDDECFKRIVAITRLAVPYTG
LIMSTRESAAMRKELLELGVSQISAGSRTAPGGYQDSKQNQHDAEQFSLGDHRAMDEIIYELVTDSDAIPSFCTGCYRKG
RTGDHFMGLAKQQFIGKFCQPNALITFREYLNDYASDKTREAGNALIERELAKMSPSRERNVRVCLKKTDAGERDIYL

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=382, Percent_Identity=31.151832460733, Blast_Score=185, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 54140; Mature: 54009

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTHEHHSITVSDYNPNVSFIDDLAIWQAIEEASNPSREQIQAILEKARQCEGLSIRETA
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHH
LLLQNQDKALDEALFAVAREIKNTIYGNRIVMFAPLYVSNHCANSCSYCGFNADNHELKR
HHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHCCCCCCCCCCCCHHHHHH
KTLKQDEIRQEVTILEEMGHKRILAVYGEHPRNNVQAIIESIQTMYSVKQGKGGEIRRIN
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
VNCAPMSVEDFKQLKTAAIGTYQCFQETYHQDTYSEVHLKGKKTDFLYRLYAMHRAMEAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHHHCC
IDDVGIGALFGLYDHRFELLAMLTHVQQLEKDCGVGPHTISFPRIEPAHGSALSEKPPYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VDDECFKRIVAITRLAVPYTGLIMSTRESAAMRKELLELGVSQISAGSRTAPGGYQDSKQ
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
NQHDAEQFSLGDHRAMDEIIYELVTDSDAIPSFCTGCYRKGRTGDHFMGLAKQQFIGKFC
CCHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
QPNALITFREYLNDYASDKTREAGNALIERELAKMSPSRERNVRVCLKKTDAGERDIYL
CCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
STHEHHSITVSDYNPNVSFIDDLAIWQAIEEASNPSREQIQAILEKARQCEGLSIRETA
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHH
LLLQNQDKALDEALFAVAREIKNTIYGNRIVMFAPLYVSNHCANSCSYCGFNADNHELKR
HHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHCCCCCCCCCCCCHHHHHH
KTLKQDEIRQEVTILEEMGHKRILAVYGEHPRNNVQAIIESIQTMYSVKQGKGGEIRRIN
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
VNCAPMSVEDFKQLKTAAIGTYQCFQETYHQDTYSEVHLKGKKTDFLYRLYAMHRAMEAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHHHCC
IDDVGIGALFGLYDHRFELLAMLTHVQQLEKDCGVGPHTISFPRIEPAHGSALSEKPPYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VDDECFKRIVAITRLAVPYTGLIMSTRESAAMRKELLELGVSQISAGSRTAPGGYQDSKQ
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
NQHDAEQFSLGDHRAMDEIIYELVTDSDAIPSFCTGCYRKGRTGDHFMGLAKQQFIGKFC
CCHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
QPNALITFREYLNDYASDKTREAGNALIERELAKMSPSRERNVRVCLKKTDAGERDIYL
CCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]