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The map label for this gene is mnmE [H]

Identifier: 117919157

GI number: 117919157

Start: 819881

End: 821119

Strand: Reverse

Name: mnmE [H]

Synonym: Shewana3_0705

Alternate gene names: 117919157

Gene position: 821119-819881 (Counterclockwise)

Preceding gene: 117919158

Following gene: 117919152

Centisome position: 16.51

GC content: 50.77

Gene sequence:

>1239_bases
ATGTGTCAAGGCATTAATCCACAAGAGCATTCCCAAAACCAATCGGCACCACGGGGCATGCGTTACCATATCGCCTTAGT
CGGTCGACGCAATGCGGGTAAGTCATCCCTGCTCAATATGATCGCAGGGCAACAAATCTCCATAGTGTCCGATATTAAGG
GCACCACGACAGATGCCGTAGCAAAGGCCTATGAGTTGCAGCCCCTAGGGCCTGTGACCTTTTACGACACGGCTGGGATC
GATGATGACGGAGAATTAGGTGCTATGCGGGTGAAGGCGACTCGCAAAGTATTGTTCCGCTCCGATATGGCACTCTTAGT
GGTGGACGAACAAGGGCTTTATCCATCGGATATTGCCTTAGTGAACGAAATACAGCAGATGCGAATGCCGATATTGGTGG
TATTCAACAAGGCCGATATCTGTACACCTAAAGCTGAAAATATCGCCTTCTGCCAACAGCAATCCTTGCCCTTTATTGTG
GTCTCGGCCACCACAGGTCTTGCGACCAAAATGCTTAAGCAGCAGATTATCGATTTAGCCCCCGCGGAATATAAGCAAGA
ACCCCTGCTAGCGGGCGACCTCTATCAATCGGGGGACGTTATTCTCTGCGTCGTACCAATCGATATGGCTGCACCTAAAG
GACGCTTGATCCTGCCGCAGGTACAAATTCTTAGGGAAGCATTGGATCGCAGCGCGATCGCTATGGTGGTGAAAGAAACT
GAACTAGAAAAGGCATTATCCGTCGTCACCCCCAAACTAGTTGTCTCCGATGCACAGGTGATTAAGCAAGTCGCCGCCCT
AGTACCAGCATCTATTCCACTCACGACTTTCTCAACTCTGTTTGCACGTTTTAAAGGGGATTTGGCCACCCTTGCCGCAG
GCGCGGAGGCGTTAGATAAATTGCAAGATGGCGATAAAGTGCTCATCGCCGAAGCCTGCAGCCACAATGTACAGGAAGAT
GATATCGGCAGAGTCAAACTCCCCCGCTGGATACATAGCTATACGGGTAAACAACTCGAATTTATCGTGACGTCCGGCCA
CGACTTTCCCGATGATTTAGAGCAATATGCCCTCGTCATCCACTGCGGTGCCTGTATGTTTAACCGCAATGAAATGCTCC
GTCGCATCCACGAATGCCAGCGTCGCAATGTGCCTATCACTAACTATGGGGTCGCGATTTCAAAGCTACAGGGCGTGCTG
CCACGGGTGCTCGCGCCTTTTAACAGCAGTCCTCAGTAA

Upstream 100 bases:

>100_bases
CAACATCCGGCTGCGGAGCGACTTAATCATATCTGCCAGCAAATACAAAGCCATGGGCTAACCCCCTCCTTTAGCCGTGG
TGATTCGAAAAGGACTCAAT

Downstream 100 bases:

>100_bases
TATTTGAGCCTTAGCTTCAGGCTTAGTTGTGATGAACTAGCTAAGCCTATTTATCCTTGATATGAAACAGATAGCCACTT
ACACAGCAATACGAGCTAAA

Product: small GTP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MCQGINPQEHSQNQSAPRGMRYHIALVGRRNAGKSSLLNMIAGQQISIVSDIKGTTTDAVAKAYELQPLGPVTFYDTAGI
DDDGELGAMRVKATRKVLFRSDMALLVVDEQGLYPSDIALVNEIQQMRMPILVVFNKADICTPKAENIAFCQQQSLPFIV
VSATTGLATKMLKQQIIDLAPAEYKQEPLLAGDLYQSGDVILCVVPIDMAAPKGRLILPQVQILREALDRSAIAMVVKET
ELEKALSVVTPKLVVSDAQVIKQVAALVPASIPLTTFSTLFARFKGDLATLAAGAEALDKLQDGDKVLIAEACSHNVQED
DIGRVKLPRWIHSYTGKQLEFIVTSGHDFPDDLEQYALVIHCGACMFNRNEMLRRIHECQRRNVPITNYGVAISKLQGVL
PRVLAPFNSSPQ

Sequences:

>Translated_412_residues
MCQGINPQEHSQNQSAPRGMRYHIALVGRRNAGKSSLLNMIAGQQISIVSDIKGTTTDAVAKAYELQPLGPVTFYDTAGI
DDDGELGAMRVKATRKVLFRSDMALLVVDEQGLYPSDIALVNEIQQMRMPILVVFNKADICTPKAENIAFCQQQSLPFIV
VSATTGLATKMLKQQIIDLAPAEYKQEPLLAGDLYQSGDVILCVVPIDMAAPKGRLILPQVQILREALDRSAIAMVVKET
ELEKALSVVTPKLVVSDAQVIKQVAALVPASIPLTTFSTLFARFKGDLATLAAGAEALDKLQDGDKVLIAEACSHNVQED
DIGRVKLPRWIHSYTGKQLEFIVTSGHDFPDDLEQYALVIHCGACMFNRNEMLRRIHECQRRNVPITNYGVAISKLQGVL
PRVLAPFNSSPQ
>Mature_412_residues
MCQGINPQEHSQNQSAPRGMRYHIALVGRRNAGKSSLLNMIAGQQISIVSDIKGTTTDAVAKAYELQPLGPVTFYDTAGI
DDDGELGAMRVKATRKVLFRSDMALLVVDEQGLYPSDIALVNEIQQMRMPILVVFNKADICTPKAENIAFCQQQSLPFIV
VSATTGLATKMLKQQIIDLAPAEYKQEPLLAGDLYQSGDVILCVVPIDMAAPKGRLILPQVQILREALDRSAIAMVVKET
ELEKALSVVTPKLVVSDAQVIKQVAALVPASIPLTTFSTLFARFKGDLATLAAGAEALDKLQDGDKVLIAEACSHNVQED
DIGRVKLPRWIHSYTGKQLEFIVTSGHDFPDDLEQYALVIHCGACMFNRNEMLRRIHECQRRNVPITNYGVAISKLQGVL
PRVLAPFNSSPQ

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Escherichia coli, GI2367268, Length=177, Percent_Identity=33.8983050847458, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 45074; Mature: 45074

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQGINPQEHSQNQSAPRGMRYHIALVGRRNAGKSSLLNMIAGQQISIVSDIKGTTTDAV
CCCCCCCHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCHHHH
AKAYELQPLGPVTFYDTAGIDDDGELGAMRVKATRKVLFRSDMALLVVDEQGLYPSDIAL
HHHHCCCCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
VNEIQQMRMPILVVFNKADICTPKAENIAFCQQQSLPFIVVSATTGLATKMLKQQIIDLA
HHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCC
PAEYKQEPLLAGDLYQSGDVILCVVPIDMAAPKGRLILPQVQILREALDRSAIAMVVKET
CCHHCCCCCEECCCCCCCCEEEEEEECCCCCCCCCEECHHHHHHHHHHCHHHHHHHHHHH
ELEKALSVVTPKLVVSDAQVIKQVAALVPASIPLTTFSTLFARFKGDLATLAAGAEALDK
HHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
LQDGDKVLIAEACSHNVQEDDIGRVKLPRWIHSYTGKQLEFIVTSGHDFPDDLEQYALVI
HCCCCEEEEEEHHCCCCCCCCCCCEECHHHHHHCCCCEEEEEEECCCCCHHHHHHHEEHE
HCGACMFNRNEMLRRIHECQRRNVPITNYGVAISKLQGVLPRVLAPFNSSPQ
ECCHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCQGINPQEHSQNQSAPRGMRYHIALVGRRNAGKSSLLNMIAGQQISIVSDIKGTTTDAV
CCCCCCCHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCHHHH
AKAYELQPLGPVTFYDTAGIDDDGELGAMRVKATRKVLFRSDMALLVVDEQGLYPSDIAL
HHHHCCCCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
VNEIQQMRMPILVVFNKADICTPKAENIAFCQQQSLPFIVVSATTGLATKMLKQQIIDLA
HHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCC
PAEYKQEPLLAGDLYQSGDVILCVVPIDMAAPKGRLILPQVQILREALDRSAIAMVVKET
CCHHCCCCCEECCCCCCCCEEEEEEECCCCCCCCCEECHHHHHHHHHHCHHHHHHHHHHH
ELEKALSVVTPKLVVSDAQVIKQVAALVPASIPLTTFSTLFARFKGDLATLAAGAEALDK
HHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
LQDGDKVLIAEACSHNVQEDDIGRVKLPRWIHSYTGKQLEFIVTSGHDFPDDLEQYALVI
HCCCCEEEEEEHHCCCCCCCCCCCEECHHHHHHCCCCEEEEEEECCCCCHHHHHHHEEHE
HCGACMFNRNEMLRRIHECQRRNVPITNYGVAISKLQGVLPRVLAPFNSSPQ
ECCHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]