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The map label for this gene is ligT [C]

Identifier: 117919072

GI number: 117919072

Start: 724189

End: 724761

Strand: Reverse

Name: ligT [C]

Synonym: Shewana3_0619

Alternate gene names: 117919072

Gene position: 724761-724189 (Counterclockwise)

Preceding gene: 117919084

Following gene: 117919070

Centisome position: 14.58

GC content: 50.79

Gene sequence:

>573_bases
GTGTTACAACGACTCTTCCTTGGGTTTGCCCCCACAAGCATACAGCGGCAACAACTGCTGCAATTGCAGCAAAGCTTGGC
GCCACATTTAGCGCCACATGCTAAACCCGTCATCCAAAGCAATTTACACTTAACCCTCGCGTTTTTAGGACTCGCCAATC
CGACTCAAGTGGCGCAGTTACTCGACGGTGTCGATAAGCTGGCTAAACCGAAATTCAGTGTCACGCTGGATAACATCGCC
GTGTGGCCTAAGGCGCAGATCTGTTGTTTAAAGGGCGATAGCATTAGTCCTGAGTTAGCCCTCTTAGCATCTCAGGCACA
GGATTTAGCCCAACAGTTACAATTGCACCTGAGTGAGCATCCGTTTCGGCCCCATATCAGCTTGTTTAGAAAGGCAAAGC
CGTCAGGCATCTTGGCGAGTGAGCGAACGCTTAAGGAGTTGGTCCAGCAGAACGTCATCCAAGCTTCGATGCTTGAACTC
ACGCTCGCACCCGAGGAGCTGCACTTATATCTTTCCGCCAGTGGCAGACGCTGTGTGGAATATCAAATCTTACATTCTTG
GCCGCTCGCATAA

Upstream 100 bases:

>100_bases
TCATCGAAGTCCATCATGGGGACTACCGCAGAAATGTCTGTATTACCAAACAAAAGCATAGCTTAAGTTTGGCATTTGAG
CAGCTGATGGAGATAAACAG

Downstream 100 bases:

>100_bases
GCCCAATCCGCTAACGCGAATAAACGATGCACTAGAAGTAATCAGTAAAAATAATCCTTAAAAAACAATCAGCCCGAGCA
TGGCTCGGGCTGATAACAAG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: 2 5' RNA Ligase; Phosphoesterase HXTX

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MLQRLFLGFAPTSIQRQQLLQLQQSLAPHLAPHAKPVIQSNLHLTLAFLGLANPTQVAQLLDGVDKLAKPKFSVTLDNIA
VWPKAQICCLKGDSISPELALLASQAQDLAQQLQLHLSEHPFRPHISLFRKAKPSGILASERTLKELVQQNVIQASMLEL
TLAPEELHLYLSASGRRCVEYQILHSWPLA

Sequences:

>Translated_190_residues
MLQRLFLGFAPTSIQRQQLLQLQQSLAPHLAPHAKPVIQSNLHLTLAFLGLANPTQVAQLLDGVDKLAKPKFSVTLDNIA
VWPKAQICCLKGDSISPELALLASQAQDLAQQLQLHLSEHPFRPHISLFRKAKPSGILASERTLKELVQQNVIQASMLEL
TLAPEELHLYLSASGRRCVEYQILHSWPLA
>Mature_190_residues
MLQRLFLGFAPTSIQRQQLLQLQQSLAPHLAPHAKPVIQSNLHLTLAFLGLANPTQVAQLLDGVDKLAKPKFSVTLDNIA
VWPKAQICCLKGDSISPELALLASQAQDLAQQLQLHLSEHPFRPHISLFRKAKPSGILASERTLKELVQQNVIQASMLEL
TLAPEELHLYLSASGRRCVEYQILHSWPLA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=188, Percent_Identity=28.7234042553192, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21081; Mature: 21081

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQRLFLGFAPTSIQRQQLLQLQQSLAPHLAPHAKPVIQSNLHLTLAFLGLANPTQVAQL
CCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEHEECCCCHHHHHHH
LDGVDKLAKPKFSVTLDNIAVWPKAQICCLKGDSISPELALLASQAQDLAQQLQLHLSEH
HHHHHHHHCCCEEEEECCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PFRPHISLFRKAKPSGILASERTLKELVQQNVIQASMLELTLAPEELHLYLSASGRRCVE
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECHHHEEEEEECCCCEEEE
YQILHSWPLA
EEEECCCCCC
>Mature Secondary Structure
MLQRLFLGFAPTSIQRQQLLQLQQSLAPHLAPHAKPVIQSNLHLTLAFLGLANPTQVAQL
CCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEHEECCCCHHHHHHH
LDGVDKLAKPKFSVTLDNIAVWPKAQICCLKGDSISPELALLASQAQDLAQQLQLHLSEH
HHHHHHHHCCCEEEEECCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PFRPHISLFRKAKPSGILASERTLKELVQQNVIQASMLELTLAPEELHLYLSASGRRCVE
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECHHHEEEEEECCCCEEEE
YQILHSWPLA
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA