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The map label for this gene is tapD [H]

Identifier: 117918874

GI number: 117918874

Start: 474440

End: 475369

Strand: Reverse

Name: tapD [H]

Synonym: Shewana3_0417

Alternate gene names: 117918874

Gene position: 475369-474440 (Counterclockwise)

Preceding gene: 117918875

Following gene: 117918873

Centisome position: 9.56

GC content: 45.81

Gene sequence:

>930_bases
ATGATGACTGACTTTATTTCTCTTTTAAGCCACTCTCTAGCTCAGAGTCCTTGGCTGTTTATTACGTTAAGTTTTGTGTT
TGCCGCCACTATTGGCAGCTTTTTAAATGTCGTCATTCACCGCTTTCCGGTGATGATGAAGCGCGAGTGGCAACAGGAGT
GCAATCAATATCTACAGGAATATCATGCAGATGTAGTAGAGCAGATTGGTATCGAAAAGCTCAACAAGCCGATAGATACT
TACCCTGAAAAATACAATCTCGTGGTGCCAGGTTCCGCCTGTCCTAAATGTAAGACCGCAATCAAACCTTGGCATAATTT
GCCGATTGTGGGCTGGTTAATGCTCAGGGGAAAATGCGCCGCCTGCAATACAGCAATCTCCTCTCGCTATCCGATTATTG
AGTTAGTAACAGGATTACTCGTTGCAACGCTCGCTTGGCATTTTGGTCCTAGCTGGCAATTTGTGTTTGCTGCGGTATTA
ACCTTTGTATTGATTGCATTAACGGGTATCGATCTGGATGAGATGCTGCTTCCCGACCAGATGACCCTTCCACTCCTATG
GCTAGGATTACTCATTAACCTCAACCATACCTTTACTACTCCAACCGATGCCGTTATAGGTGCTGCAGCGGGCTATTTGA
GTCTCTGGTCAGTATTTTGGCTGTTTAAGTTGCTCACAGGTAAAGAAGGCATGGGTTATGGCGACTTTAAACTGCTCGCC
GTATTTGGTGCATGGCTAGGTTGGCAAATGCTCCCCCTAGTCATTTTACTATCCTCTTTAGTTGGTGCCCTTGTCGGCAT
CACGCTCATCGTACTCAAACGTAATCAGCTAGCCAATCCAATCCCCTTTGGGCCCTATATTGCCGCTGCAGGTTGGATAG
CCCTGATATGGGGACAACCTATAGTCGATTGGTATCTAAGTACGCTATAA

Upstream 100 bases:

>100_bases
TTACCAATCTTCCAAATGGGTAAAGTTGTAGGTGGTTAAGCCGTTGTAGACTCAATGTTTTCCAGAATTGAAACTGTACT
TTTAATAATAACAAGTAACA

Downstream 100 bases:

>100_bases
AAACACTGTCTATATCTTTCTTCAGTGTTTAGCATTCAATTTTGTACAGATATAGTCCAAATTGATTAGGTGCCTTATCT
AGATGGGAATCTTATGTCCA

Product: type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MMTDFISLLSHSLAQSPWLFITLSFVFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHADVVEQIGIEKLNKPIDT
YPEKYNLVVPGSACPKCKTAIKPWHNLPIVGWLMLRGKCAACNTAISSRYPIIELVTGLLVATLAWHFGPSWQFVFAAVL
TFVLIALTGIDLDEMLLPDQMTLPLLWLGLLINLNHTFTTPTDAVIGAAAGYLSLWSVFWLFKLLTGKEGMGYGDFKLLA
VFGAWLGWQMLPLVILLSSLVGALVGITLIVLKRNQLANPIPFGPYIAAAGWIALIWGQPIVDWYLSTL

Sequences:

>Translated_309_residues
MMTDFISLLSHSLAQSPWLFITLSFVFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHADVVEQIGIEKLNKPIDT
YPEKYNLVVPGSACPKCKTAIKPWHNLPIVGWLMLRGKCAACNTAISSRYPIIELVTGLLVATLAWHFGPSWQFVFAAVL
TFVLIALTGIDLDEMLLPDQMTLPLLWLGLLINLNHTFTTPTDAVIGAAAGYLSLWSVFWLFKLLTGKEGMGYGDFKLLA
VFGAWLGWQMLPLVILLSSLVGALVGITLIVLKRNQLANPIPFGPYIAAAGWIALIWGQPIVDWYLSTL
>Mature_309_residues
MMTDFISLLSHSLAQSPWLFITLSFVFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHADVVEQIGIEKLNKPIDT
YPEKYNLVVPGSACPKCKTAIKPWHNLPIVGWLMLRGKCAACNTAISSRYPIIELVTGLLVATLAWHFGPSWQFVFAAVL
TFVLIALTGIDLDEMLLPDQMTLPLLWLGLLINLNHTFTTPTDAVIGAAAGYLSLWSVFWLFKLLTGKEGMGYGDFKLLA
VFGAWLGWQMLPLVILLSSLVGALVGITLIVLKRNQLANPIPFGPYIAAAGWIALIWGQPIVDWYLSTL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=278, Percent_Identity=33.8129496402878, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=48.4848484848485, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 34454; Mature: 34454

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTDFISLLSHSLAQSPWLFITLSFVFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHADVVEQIGIEKLNKPIDTYPEKYNLVVPGSACPKCKTAIKPWHNLPIVGWLMLRGKCA
HHHHHHHHHCHHHHCCCHHCCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHHHCCCHH
ACNTAISSRYPIIELVTGLLVATLAWHFGPSWQFVFAAVLTFVLIALTGIDLDEMLLPDQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
MTLPLLWLGLLINLNHTFTTPTDAVIGAAAGYLSLWSVFWLFKLLTGKEGMGYGDFKLLA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VFGAWLGWQMLPLVILLSSLVGALVGITLIVLKRNQLANPIPFGPYIAAAGWIALIWGQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCH
IVDWYLSTL
HHHHHHHCC
>Mature Secondary Structure
MMTDFISLLSHSLAQSPWLFITLSFVFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHADVVEQIGIEKLNKPIDTYPEKYNLVVPGSACPKCKTAIKPWHNLPIVGWLMLRGKCA
HHHHHHHHHCHHHHCCCHHCCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHHHCCCHH
ACNTAISSRYPIIELVTGLLVATLAWHFGPSWQFVFAAVLTFVLIALTGIDLDEMLLPDQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
MTLPLLWLGLLINLNHTFTTPTDAVIGAAAGYLSLWSVFWLFKLLTGKEGMGYGDFKLLA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VFGAWLGWQMLPLVILLSSLVGALVGITLIVLKRNQLANPIPFGPYIAAAGWIALIWGQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCH
IVDWYLSTL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA