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The map label for this gene is oprF [H]

Identifier: 117918835

GI number: 117918835

Start: 419722

End: 420330

Strand: Reverse

Name: oprF [H]

Synonym: Shewana3_0378

Alternate gene names: 117918835

Gene position: 420330-419722 (Counterclockwise)

Preceding gene: 117918836

Following gene: 117918834

Centisome position: 8.45

GC content: 47.29

Gene sequence:

>609_bases
ATGAAAACCGTAATCCTATTTATGAGCGTGGCACTTTTAGCTGGTTGTTCCATGCGGGATACCGTTAATATTGAAGAAAT
GACCACGACTCAGGCCTATGATCTGTCCGACAAGGATGGTGATGGCGTGATTGAAGCGCGTGAGCGTTGCAACGACACCG
CCAAAGGCGCTGGTACGGATAATTATGGCTGCGGTCAAATAAAAGCAATTAATGAGCGTAAGGAAATCAATGTATTATTT
CCAAACGACTCAGCTTATATTGCACCAGAGTACTATCCACAGATTGAAGAAATTGCGATGTTTTTACGTCAATATCCCAC
GACTAAAGTGACTATCGAAGGTCACACTAGCCGCACTGGGACAGATGAAAGAAACGCCGTACTTTCTCAAGAACGTGCTG
ATGCCGTCACCGCGGTGCTAGCAGACCGTTTCAGCATCGACAGATCTAGACTCACAGCCATTGGCTACGGCTCTAGCCGT
CCATTGGTACTCGAGCATACGCCTGATGCTGAAACCCGTAACCGCCGCGTAGTCGCGGAAGTGACCGGTGATGATACGAC
GACAGATATGAAATGGACGATTTACTCTGTCGATGAACCCACTAAATAA

Upstream 100 bases:

>100_bases
ATCGCGTACTCAACTCTACTGGACGTTTGCTTGACTCCTTAAAAGTCACTCGCCCAGAAGCATGGCGTGGTGAACGCAAC
TACGAGGGAGGAGCTAACCA

Downstream 100 bases:

>100_bases
AGGCTAACAGACTAGGCTATGAACGGCAGGCTAAGGGATTACAATTCGTTTTTGCGGGCCTGTCGCTTAAGCCTAATCGC
GTCTGCGCTTGCGGTCGGCT

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MKTVILFMSVALLAGCSMRDTVNIEEMTTTQAYDLSDKDGDGVIEARERCNDTAKGAGTDNYGCGQIKAINERKEINVLF
PNDSAYIAPEYYPQIEEIAMFLRQYPTTKVTIEGHTSRTGTDERNAVLSQERADAVTAVLADRFSIDRSRLTAIGYGSSR
PLVLEHTPDAETRNRRVVAEVTGDDTTTDMKWTIYSVDEPTK

Sequences:

>Translated_202_residues
MKTVILFMSVALLAGCSMRDTVNIEEMTTTQAYDLSDKDGDGVIEARERCNDTAKGAGTDNYGCGQIKAINERKEINVLF
PNDSAYIAPEYYPQIEEIAMFLRQYPTTKVTIEGHTSRTGTDERNAVLSQERADAVTAVLADRFSIDRSRLTAIGYGSSR
PLVLEHTPDAETRNRRVVAEVTGDDTTTDMKWTIYSVDEPTK
>Mature_202_residues
MKTVILFMSVALLAGCSMRDTVNIEEMTTTQAYDLSDKDGDGVIEARERCNDTAKGAGTDNYGCGQIKAINERKEINVLF
PNDSAYIAPEYYPQIEEIAMFLRQYPTTKVTIEGHTSRTGTDERNAVLSQERADAVTAVLADRFSIDRSRLTAIGYGSSR
PLVLEHTPDAETRNRRVVAEVTGDDTTTDMKWTIYSVDEPTK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=101, Percent_Identity=36.6336633663366, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786962, Length=101, Percent_Identity=35.6435643564356, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 22444; Mature: 22444

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVILFMSVALLAGCSMRDTVNIEEMTTTQAYDLSDKDGDGVIEARERCNDTAKGAGTD
CCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCC
NYGCGQIKAINERKEINVLFPNDSAYIAPEYYPQIEEIAMFLRQYPTTKVTIEGHTSRTG
CCCCCEEEEECCCCEEEEEECCCCCEECCHHCCCHHHHHHHHHHCCCEEEEEECCCCCCC
TDERNAVLSQERADAVTAVLADRFSIDRSRLTAIGYGSSRPLVLEHTPDAETRNRRVVAE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCEEEEECCCCCCCCCEEEEE
VTGDDTTTDMKWTIYSVDEPTK
ECCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKTVILFMSVALLAGCSMRDTVNIEEMTTTQAYDLSDKDGDGVIEARERCNDTAKGAGTD
CCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCC
NYGCGQIKAINERKEINVLFPNDSAYIAPEYYPQIEEIAMFLRQYPTTKVTIEGHTSRTG
CCCCCEEEEECCCCEEEEEECCCCCEECCHHCCCHHHHHHHHHHCCCEEEEEECCCCCCC
TDERNAVLSQERADAVTAVLADRFSIDRSRLTAIGYGSSRPLVLEHTPDAETRNRRVVAE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCEEEEECCCCCCCCCEEEEE
VTGDDTTTDMKWTIYSVDEPTK
ECCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]