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The map label for this gene is dctA [H]

Identifier: 117918754

GI number: 117918754

Start: 329865

End: 331109

Strand: Reverse

Name: dctA [H]

Synonym: Shewana3_0297

Alternate gene names: 117918754

Gene position: 331109-329865 (Counterclockwise)

Preceding gene: 117918755

Following gene: 117918753

Centisome position: 6.66

GC content: 50.04

Gene sequence:

>1245_bases
ATGACTCACCGAAACAATGATAAAAATAATAGGCGCTTAGCTATGTGGCAAAAACTCAAACCCTATCAAGACTCTATTGT
GCTACTCAGCGCATTATTACTCGGCGGCATTATCGGCATCTATTGGCCATCCTTGGCACTCAAGCTCAAGCCATTAGGGC
AAATCTTTCTGAATCTGTTGTTCATGATTATCGTGCCCTTGGTGGCGATCAGCGTGACCTCATCAATAGCCCGCATGACG
GACTTAAAAAAGCTCGGGGCGATTTTAGCGTCGATTATGGGCGTCTCGATTCTGATGGCGATTATTCCCGCCGTCGGCAT
CACCTTACTAGCATTGGTGTTTGATCCAGCCCAAGGCGTGACCCTCGATCTTAATCAAACCGTTAGCGATACCTCGGGGA
AGATGGATTTTGTGGCGCTGCTGACCACTAATGACTTTTCTGGATTACTCTCCAAATCCAATATCTTGGCGCTTATCATC
ATGTCGGTGATTGCCGGAATCGCCATAGGTCAATCGGGGGAAGATGGCCGTAAAGTTTCGGCACTGCTCGACAGTTTTAA
TACCGTGATCATGAAGATTGTCAGCATTATTATGCTGGCAGCCCCCGTCGGCCTTGGCGCTTACTTTGCCTCCACCATGG
CGAGTCAAGATCCGCAGCTTGTGGTGACCTTTGCCCGCGCCGTAGGATTATTTTTAGTGGCCTGTTTTATCTACCTTACC
GTCGGCTCCACCTTTTATGCCTGGCTTGGTGGTGGCATGCACGGGATTAGACAATTTTGGCGACACGCCCTCGAGCCCGC
AGTGACTGCATTAGGTACTACCTCCTCCCTCGCGACCCTGCCCGTCACCTTAAGGGCGGCGCTGAAGATGGGGATAAAAC
CCGAAATAGCCGATATCAGCCTGCCGCTGCTGGTGAATTTAAATAAGGGCGGCGCATCCATTATCACCGCCTTGAAGATT
GTATTTATTTACTCTCTGCTCGGTTTAGATTTTACGGTCGATGTGTTTATGGTGACCATACTCATCTCAGTCCTGTCCGC
CTTCATCATTGGCGGCGTGCCGGGCGGCGCTTTTCTCGGGGAGATTTTTATCGTCACTGCCTTAGGGTTACCAATGGAAA
CCATCCCCATTTTGGTGGTGATAGGCGCCATTACCGACGCACCTTCCACCGTGATAAACGTCGTCCATGATCTGAATGCC
AGCCAGATTGTTGAACGCCTCAACTGCAAACGGATTGAGTCATAA

Upstream 100 bases:

>100_bases
AACAGTTTATTTCCAATCCCTTCTTGCACTTATTTACATGACTCGCTAGCTTGAGCATGTCCATGCTGCATGATTGTTCC
CTTTCATTTGGGCGCTCCTA

Downstream 100 bases:

>100_bases
CAGGGCAATTGCCAAATAATTGACAACGAAACTCATCTGATTAGAATGCCAAACCATTGAAACAGAGCACATTTGTGCCG
AGTAAAATCCGCTATGCTGC

Product: sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTHRNNDKNNRRLAMWQKLKPYQDSIVLLSALLLGGIIGIYWPSLALKLKPLGQIFLNLLFMIIVPLVAISVTSSIARMT
DLKKLGAILASIMGVSILMAIIPAVGITLLALVFDPAQGVTLDLNQTVSDTSGKMDFVALLTTNDFSGLLSKSNILALII
MSVIAGIAIGQSGEDGRKVSALLDSFNTVIMKIVSIIMLAAPVGLGAYFASTMASQDPQLVVTFARAVGLFLVACFIYLT
VGSTFYAWLGGGMHGIRQFWRHALEPAVTALGTTSSLATLPVTLRAALKMGIKPEIADISLPLLVNLNKGGASIITALKI
VFIYSLLGLDFTVDVFMVTILISVLSAFIIGGVPGGAFLGEIFIVTALGLPMETIPILVVIGAITDAPSTVINVVHDLNA
SQIVERLNCKRIES

Sequences:

>Translated_414_residues
MTHRNNDKNNRRLAMWQKLKPYQDSIVLLSALLLGGIIGIYWPSLALKLKPLGQIFLNLLFMIIVPLVAISVTSSIARMT
DLKKLGAILASIMGVSILMAIIPAVGITLLALVFDPAQGVTLDLNQTVSDTSGKMDFVALLTTNDFSGLLSKSNILALII
MSVIAGIAIGQSGEDGRKVSALLDSFNTVIMKIVSIIMLAAPVGLGAYFASTMASQDPQLVVTFARAVGLFLVACFIYLT
VGSTFYAWLGGGMHGIRQFWRHALEPAVTALGTTSSLATLPVTLRAALKMGIKPEIADISLPLLVNLNKGGASIITALKI
VFIYSLLGLDFTVDVFMVTILISVLSAFIIGGVPGGAFLGEIFIVTALGLPMETIPILVVIGAITDAPSTVINVVHDLNA
SQIVERLNCKRIES
>Mature_413_residues
THRNNDKNNRRLAMWQKLKPYQDSIVLLSALLLGGIIGIYWPSLALKLKPLGQIFLNLLFMIIVPLVAISVTSSIARMTD
LKKLGAILASIMGVSILMAIIPAVGITLLALVFDPAQGVTLDLNQTVSDTSGKMDFVALLTTNDFSGLLSKSNILALIIM
SVIAGIAIGQSGEDGRKVSALLDSFNTVIMKIVSIIMLAAPVGLGAYFASTMASQDPQLVVTFARAVGLFLVACFIYLTV
GSTFYAWLGGGMHGIRQFWRHALEPAVTALGTTSSLATLPVTLRAALKMGIKPEIADISLPLLVNLNKGGASIITALKIV
FIYSLLGLDFTVDVFMVTILISVLSAFIIGGVPGGAFLGEIFIVTALGLPMETIPILVVIGAITDAPSTVINVVHDLNAS
QIVERLNCKRIES

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=274, Percent_Identity=28.1021897810219, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI66773030, Length=267, Percent_Identity=29.5880149812734, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI40254478, Length=310, Percent_Identity=27.0967741935484, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI169790839, Length=281, Percent_Identity=26.6903914590747, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI223468564, Length=263, Percent_Identity=28.5171102661597, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI223468566, Length=263, Percent_Identity=28.5171102661597, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI21314632, Length=416, Percent_Identity=25.9615384615385, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI5032093, Length=264, Percent_Identity=28.7878787878788, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI194239697, Length=268, Percent_Identity=26.865671641791, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI262359914, Length=189, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789947, Length=408, Percent_Identity=25.7352941176471, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790514, Length=430, Percent_Identity=25.8139534883721, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788024, Length=381, Percent_Identity=22.5721784776903, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17537407, Length=386, Percent_Identity=24.3523316062176, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=456, Percent_Identity=24.780701754386, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71996953, Length=273, Percent_Identity=24.5421245421245, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71983099, Length=449, Percent_Identity=21.6035634743875, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983106, Length=449, Percent_Identity=21.6035634743875, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193206654, Length=262, Percent_Identity=25.9541984732824, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17541374, Length=404, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583025, Length=460, Percent_Identity=22.6086956521739, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137668, Length=460, Percent_Identity=22.6086956521739, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583023, Length=460, Percent_Identity=22.6086956521739, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 43962; Mature: 43831

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRNNDKNNRRLAMWQKLKPYQDSIVLLSALLLGGIIGIYWPSLALKLKPLGQIFLNLL
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHH
FMIIVPLVAISVTSSIARMTDLKKLGAILASIMGVSILMAIIPAVGITLLALVFDPAQGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TLDLNQTVSDTSGKMDFVALLTTNDFSGLLSKSNILALIIMSVIAGIAIGQSGEDGRKVS
EEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ALLDSFNTVIMKIVSIIMLAAPVGLGAYFASTMASQDPQLVVTFARAVGLFLVACFIYLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VGSTFYAWLGGGMHGIRQFWRHALEPAVTALGTTSSLATLPVTLRAALKMGIKPEIADIS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
LPLLVNLNKGGASIITALKIVFIYSLLGLDFTVDVFMVTILISVLSAFIIGGVPGGAFLG
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EIFIVTALGLPMETIPILVVIGAITDAPSTVINVVHDLNASQIVERLNCKRIES
HHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
THRNNDKNNRRLAMWQKLKPYQDSIVLLSALLLGGIIGIYWPSLALKLKPLGQIFLNLL
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHH
FMIIVPLVAISVTSSIARMTDLKKLGAILASIMGVSILMAIIPAVGITLLALVFDPAQGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TLDLNQTVSDTSGKMDFVALLTTNDFSGLLSKSNILALIIMSVIAGIAIGQSGEDGRKVS
EEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ALLDSFNTVIMKIVSIIMLAAPVGLGAYFASTMASQDPQLVVTFARAVGLFLVACFIYLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VGSTFYAWLGGGMHGIRQFWRHALEPAVTALGTTSSLATLPVTLRAALKMGIKPEIADIS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
LPLLVNLNKGGASIITALKIVFIYSLLGLDFTVDVFMVTILISVLSAFIIGGVPGGAFLG
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EIFIVTALGLPMETIPILVVIGAITDAPSTVINVVHDLNASQIVERLNCKRIES
HHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA