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The map label for this gene is trmA [H]

Identifier: 117918637

GI number: 117918637

Start: 212709

End: 213806

Strand: Reverse

Name: trmA [H]

Synonym: Shewana3_0179

Alternate gene names: 117918637

Gene position: 213806-212709 (Counterclockwise)

Preceding gene: 117918639

Following gene: 117918635

Centisome position: 4.3

GC content: 45.9

Gene sequence:

>1098_bases
ATGAACTTAGCAGCAATGGATCCCACGACCTATGATGCGCAACTAACGGCAAAGCGTATCAAGCTAGAGCAAGCCTTTGC
CCAATTTGAGACACCGAGTGTTGAAGTGTTTGCCTCAGAACCTGCCCACTATCGGATGCGCGCCGAGTTTCGCGTATGGC
ACGAGGGCGACGATTTATACTATTACATGTTCGATAAAGTATTGAACGACAAAGTTCGTTGCGATCAATACTTACCCGCG
AGTGCCTTAATCAATCAGATGATGGCGGCTCTGATCACCGAGTTAAAACCGAACCACAGCCTACGCCATAAACTCTTCCA
AGTTGATTTTTTATCAACGCTCAGTGGTGAGATTTTAGTGTCACTGCTGTATCACAGACAGCTTGATGATCAATGGCGCA
GCGAAGCCGCTGCACTTAAAGCCAGATTGAGTAGCCAATTTAAGGTCAATATCATTGGCCGTGCGCGCAAACAGAAAATC
GATTTAGATAAAGACTTCGTGGTTGAGTCACTGCAAGTGAATGACAAAGTTTTCCATTACAAACAAATCGAAAACAGCTT
TACCCAGCCAAATGCCAAAGTGGCTATCAAGATGCTGGAGTGGGCTATCGACGTGACTCAAAATAGCCAAGGGGATTTAC
TGGAGCTTTACTGTGGTAATGGCAACTTCTCCATCGCGCTGGCACAAAACTTTAATCGCGTGCTCGCAACCGAGTTGGCT
AAACCTTCAGTCGATGCTGCCCAGTACAATATCGAAGTCAACAATATCGATAATCTGCAGATTATCCGTATGTCGGCGGA
AGAATTTAGCGATGCGATGGCGAAAAAGCGCAGCTTTAGACGCTTAGAAGGCATAGATCTGGATAGCTATGTCTGCAACA
CCATTTTTGTCGACCCACCACGAGCAGGTATCGACCCTGCCACGTTGGAGTTAGTGCGAGGTTATGAGCGGATCCTGTAT
ATCTCTTGTAATCCCGACACCTTGAAAGACAATTTGCAGCAATTAAATCAGACTCATAAAGTCACCCGTTTTGCCCTGTT
TGATCAGTTCCCTTATACCGATCATATGGAAACAGGTGTACTGCTTGAGCGTCGTTAA

Upstream 100 bases:

>100_bases
TTTCCCACAGCTTTGCTCAAACCGCAAAACCAAGATGCTGGACATTCGTGTCACCTAGTATAAAATTTGCGCCCCAATTT
ACAGCCACGGTGAATCGACA

Downstream 100 bases:

>100_bases
GCGACTCTATGTACCTAAAAATAAAGGAGCCAATGGCTCCTTTATTTTTGGGTTAAATCACAATTTCACTTAACGATGCT
CAACCTTATGTTGTAAGGCT

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNLAAMDPTTYDAQLTAKRIKLEQAFAQFETPSVEVFASEPAHYRMRAEFRVWHEGDDLYYYMFDKVLNDKVRCDQYLPA
SALINQMMAALITELKPNHSLRHKLFQVDFLSTLSGEILVSLLYHRQLDDQWRSEAAALKARLSSQFKVNIIGRARKQKI
DLDKDFVVESLQVNDKVFHYKQIENSFTQPNAKVAIKMLEWAIDVTQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
KPSVDAAQYNIEVNNIDNLQIIRMSAEEFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPPRAGIDPATLELVRGYERILY
ISCNPDTLKDNLQQLNQTHKVTRFALFDQFPYTDHMETGVLLERR

Sequences:

>Translated_365_residues
MNLAAMDPTTYDAQLTAKRIKLEQAFAQFETPSVEVFASEPAHYRMRAEFRVWHEGDDLYYYMFDKVLNDKVRCDQYLPA
SALINQMMAALITELKPNHSLRHKLFQVDFLSTLSGEILVSLLYHRQLDDQWRSEAAALKARLSSQFKVNIIGRARKQKI
DLDKDFVVESLQVNDKVFHYKQIENSFTQPNAKVAIKMLEWAIDVTQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
KPSVDAAQYNIEVNNIDNLQIIRMSAEEFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPPRAGIDPATLELVRGYERILY
ISCNPDTLKDNLQQLNQTHKVTRFALFDQFPYTDHMETGVLLERR
>Mature_365_residues
MNLAAMDPTTYDAQLTAKRIKLEQAFAQFETPSVEVFASEPAHYRMRAEFRVWHEGDDLYYYMFDKVLNDKVRCDQYLPA
SALINQMMAALITELKPNHSLRHKLFQVDFLSTLSGEILVSLLYHRQLDDQWRSEAAALKARLSSQFKVNIIGRARKQKI
DLDKDFVVESLQVNDKVFHYKQIENSFTQPNAKVAIKMLEWAIDVTQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
KPSVDAAQYNIEVNNIDNLQIIRMSAEEFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPPRAGIDPATLELVRGYERILY
ISCNPDTLKDNLQQLNQTHKVTRFALFDQFPYTDHMETGVLLERR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=261, Percent_Identity=24.904214559387, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI51173878, Length=261, Percent_Identity=24.904214559387, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790403, Length=353, Percent_Identity=61.7563739376771, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1789148, Length=185, Percent_Identity=31.8918918918919, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24666579, Length=192, Percent_Identity=29.1666666666667, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 42053; Mature: 42053

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAAMDPTTYDAQLTAKRIKLEQAFAQFETPSVEVFASEPAHYRMRAEFRVWHEGDDLY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEEEECCCCEE
YYMFDKVLNDKVRCDQYLPASALINQMMAALITELKPNHSLRHKLFQVDFLSTLSGEILV
EEEHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDDQWRSEAAALKARLSSQFKVNIIGRARKQKIDLDKDFVVESLQVNDKVFHY
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCCHHHHHHHHHCCCHHHHH
KQIENSFTQPNAKVAIKMLEWAIDVTQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHC
KPSVDAAQYNIEVNNIDNLQIIRMSAEEFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPP
CCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHEEECEEEECCC
RAGIDPATLELVRGYERILYISCNPDTLKDNLQQLNQTHKVTRFALFDQFPYTDHMETGV
CCCCCHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LLERR
EEECC
>Mature Secondary Structure
MNLAAMDPTTYDAQLTAKRIKLEQAFAQFETPSVEVFASEPAHYRMRAEFRVWHEGDDLY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEEEECCCCEE
YYMFDKVLNDKVRCDQYLPASALINQMMAALITELKPNHSLRHKLFQVDFLSTLSGEILV
EEEHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDDQWRSEAAALKARLSSQFKVNIIGRARKQKIDLDKDFVVESLQVNDKVFHY
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCCHHHHHHHHHCCCHHHHH
KQIENSFTQPNAKVAIKMLEWAIDVTQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHC
KPSVDAAQYNIEVNNIDNLQIIRMSAEEFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPP
CCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHEEECEEEECCC
RAGIDPATLELVRGYERILYISCNPDTLKDNLQQLNQTHKVTRFALFDQFPYTDHMETGV
CCCCCHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LLERR
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]