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The map label for this gene is rimN [H]

Identifier: 117918499

GI number: 117918499

Start: 46495

End: 47061

Strand: Direct

Name: rimN [H]

Synonym: Shewana3_0040

Alternate gene names: 117918499

Gene position: 46495-47061 (Clockwise)

Preceding gene: 117918498

Following gene: 117918500

Centisome position: 0.94

GC content: 50.09

Gene sequence:

>567_bases
ATGTTACAGCTGCACCCATCTGAAATTAAAGACTTAGTGCTTAACGGTGGCGTTATCGCCTATCCCACTGAAGCCGTTTA
TGGTTTAGGCTGCGATCCCGACAACGACACCGCCATCCAAAAATTACTCGCGGTAAAGCAACGTCCTTGGCAGAAGGGGC
TTATCTTAGTGGCGAGCGAGTTTTCCCAGTTAGTGGATTATGTCGATGAGTCACAGCTGAGCGCCGAGCAACTCGAGTTT
GCCTTTTCTAAATGGCCTGGCCCCTTTACCTTTGTGATGCCGATTAAGCCGCATGTTTCGCGCTATTTATGTGGTGAGTT
TGATTCTATCGCCGTGCGCGTCTCCGCCCATGACGGAGTGCGCGCTTTATGCCAAGCCTTAGGTAAACCACTGGTTTCGA
CTAGCGCCAATTTAGCCGGCGAAGATCCTGCGCTGAGCGGTGATGAAATTCTTAATGCCTTTGAGGGTAAGATTGATGCC
TTAGTGTTAGGGGCATTAGGCGAGCAGCGTCAACCCTCCACCATTATTGATGCCCGCAGCGGTAAGATATTACGTAACGG
ACAATAA

Upstream 100 bases:

>100_bases
TAAACACCCTTGGGTATAATCCCCCATCGACCACGGATGGGGTGGCCAAGGGCTAAAACGTGTTTGTAGAAATCGATTCA
ATTCATAAGGTGATATTAAG

Downstream 100 bases:

>100_bases
CTGATTTATAACGCAAAGGATAAAAGCAGATGAGCGTGCCAGATGCGACAGTGGTAAAAGCATTTTTACTCGATCTACAG
AATCGAATTTGTGCGGGTCT

Product: translation factor SUA5

Products: NA

Alternate protein names: t(6)A37 threonylcarbamoyladenosine biosynthesis protein RimN [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MLQLHPSEIKDLVLNGGVIAYPTEAVYGLGCDPDNDTAIQKLLAVKQRPWQKGLILVASEFSQLVDYVDESQLSAEQLEF
AFSKWPGPFTFVMPIKPHVSRYLCGEFDSIAVRVSAHDGVRALCQALGKPLVSTSANLAGEDPALSGDEILNAFEGKIDA
LVLGALGEQRQPSTIIDARSGKILRNGQ

Sequences:

>Translated_188_residues
MLQLHPSEIKDLVLNGGVIAYPTEAVYGLGCDPDNDTAIQKLLAVKQRPWQKGLILVASEFSQLVDYVDESQLSAEQLEF
AFSKWPGPFTFVMPIKPHVSRYLCGEFDSIAVRVSAHDGVRALCQALGKPLVSTSANLAGEDPALSGDEILNAFEGKIDA
LVLGALGEQRQPSTIIDARSGKILRNGQ
>Mature_188_residues
MLQLHPSEIKDLVLNGGVIAYPTEAVYGLGCDPDNDTAIQKLLAVKQRPWQKGLILVASEFSQLVDYVDESQLSAEQLEF
AFSKWPGPFTFVMPIKPHVSRYLCGEFDSIAVRVSAHDGVRALCQALGKPLVSTSANLAGEDPALSGDEILNAFEGKIDA
LVLGALGEQRQPSTIIDARSGKILRNGQ

Specific function: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine [H]

COG id: COG0009

COG function: function code J; Putative translation factor (SUA5)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367210, Length=177, Percent_Identity=51.9774011299435, Blast_Score=180, Evalue=5e-47,
Organism=Escherichia coli, GI87081864, Length=169, Percent_Identity=31.3609467455621, Blast_Score=84, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321269, Length=174, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI78707274, Length=173, Percent_Identity=33.5260115606936, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR004388
- InterPro:   IPR006070 [H]

Pfam domain/function: PF01300 Sua5_yciO_yrdC [H]

EC number: NA

Molecular weight: Translated: 20305; Mature: 20305

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLHPSEIKDLVLNGGVIAYPTEAVYGLGCDPDNDTAIQKLLAVKQRPWQKGLILVASE
CCCCCHHHHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEHHH
FSQLVDYVDESQLSAEQLEFAFSKWPGPFTFVMPIKPHVSRYLCGEFDSIAVRVSAHDGV
HHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCH
RALCQALGKPLVSTSANLAGEDPALSGDEILNAFEGKIDALVLGALGEQRQPSTIIDARS
HHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHCCHHHEEEEECCCCCCCCCEEEECCC
GKILRNGQ
CCEEECCC
>Mature Secondary Structure
MLQLHPSEIKDLVLNGGVIAYPTEAVYGLGCDPDNDTAIQKLLAVKQRPWQKGLILVASE
CCCCCHHHHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEHHH
FSQLVDYVDESQLSAEQLEFAFSKWPGPFTFVMPIKPHVSRYLCGEFDSIAVRVSAHDGV
HHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCH
RALCQALGKPLVSTSANLAGEDPALSGDEILNAFEGKIDALVLGALGEQRQPSTIIDARS
HHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHCCHHHEEEEECCCCCCCCCEEEECCC
GKILRNGQ
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]