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The map label for this gene is gyrB [H]

Identifier: 117918471

GI number: 117918471

Start: 13416

End: 15833

Strand: Direct

Name: gyrB [H]

Synonym: Shewana3_0012

Alternate gene names: 117918471

Gene position: 13416-15833 (Clockwise)

Preceding gene: 117918470

Following gene: 117918473

Centisome position: 0.27

GC content: 48.51

Gene sequence:

>2418_bases
ATGTCAGAGAATAGTTACGATTCTTCGAGTATTAAGGTCCTTAAGGGCCTTGATGCAGTACGTAAGAGACCTGGGATGTA
TATTGGTGACACCGACGACGGTACGGGTCTACATCATATGGTGTTCGAAGTAGTCGATAACTCTATCGATGAAGCCTTAG
CCGGCCATTGTACTGATATCACTATTACTATCCATGTGGATGGCTCGGTCTCCGTTCGCGACGACGGCCGTGGTATTCCT
GTGGCGATTCACCCGGAAGAAGGCGTGTCTGCCGCCGAGGTGATCATGACCGTATTGCACGCGGGCGGTAAGTTCGACGA
TAACTCTTATAAAGTGTCAGGTGGTCTGCACGGCGTGGGTGTGTCGGTAGTTAACGCGCTGTCTAAAAAGCTGCAGTTAA
CCATTCGCCGTGAAGGTAAAGTCCACGAGCAGTTTTATACCCACGGTGTTCCTGACGAGCCAATCAAAGTGATCGGCGAT
GCGACGAAAACCGGTACCGAAATCCGTTTCTGGCCAAGTGAAGAAACCTTCAGCGATGTTGAGTTCCACTTCGAGATTCT
GGCAAAGCGCGTGCGCGAACTGTCGTTCCTCAACTCAGGCGTGGGTATCCGCTTAGTCGACGAGCGTGACAATAAGAATG
AATTCTTTAAATACGAAGGTGGTATCAGCGCATTCGTTGATTACCTGAACCGTAATAAAACGCCAGTAAACAAAGACGTA
TTCCACTTTATGCAAGAGCGTGACGACGGTATTACCGTTGAAGTGGCGATGCAGTGGAACGATGGTTATCAAGAAAGCAT
TTTCTGTTTTACCAACAACATTCCACAGCGTGATGGTGGTACTCACTTAGCGGGTTTCCGTAGTGCGTTGACACGTAACC
TCAACAACTACATGGAAAACGAAGGCTATAACAAGAAAGGCAAAACTAACGCGACTGGCGATGACGCCCGCGAAGGTTTA
ACGGCCGTGATTTCAGTTAAAGTGCCTGATCCTAAGTTCAGCTCGCAAACCAAAGACAAGTTAGTGTCTAGTGAAGTGAA
AGCCGCGGTTGAACAAACCATGGGTGAAAAGCTAAACGACTATCTGCTGGAAAACCCTGCCGATGCTAAGTTAATCGTCG
GTAAGATTGTCGATGCGGCCCGTGCCCGTGAAGCGGCACGTAAAGCCCGTGAAATGACCCGTCGTAAAGGCGCACTCGAT
TTAGGTGGTTTGCCAGGTAAGCTGGCGGATTGCCAAGAGAAAGACCCTGGTCTTTCAGAAATCTACATAGTGGAAGGGGA
CTCTGCTGGCGGTAGCGCTAAGCAGGGACGTAACCGTAAGAACCAAGCGATTCTGCCACTGAAAGGTAAAATCTTAAACG
TTGAGAAAGCGCGTTTCGATAAAATGCTTTCTTCTCAAGAGGTTGCCACGCTAATTACTGCACTTGGCTGTGGTATTGGT
CGTGACGAATACAACCCGGATAAGACGCGTTACCACAACATCATCATCATGACGGATGCTGACGTCGACGGCTCGCATAT
TCGTACCTTGCTGTTGACCTTCTTCTTCCGCCAAATGCCTGAACTGATTGAACGTGGTTATGTGTATATTGCTCAGCCGC
CTTTATTCAAAGTGAAGAAAGGTAAGCAAGAACAATATCTCAAAGACGAAGCGGCACTGACTCAGTATCTGACCACTCAA
GCGTTAGATGGTACTAGTATTTATCCATCTCAAGGTGCGCCTGGTATGTCGGGTGAGCCATTAGAGCGTTTAGTGACTCA
ATACCGCGAAGTGGAAGCGATTGTGGCCCGTTTAGAGCAACGTTACCCTACTCAGATCACCAACCGCATGCTTTATCATC
CAATGATCAACAACGAAATGTTGGCGGATGAAGGCAAGATGAAGGAATGTATCGACGCCTTTATCCATGAGCTGGTGGAA
TTAGAAAACAGCGGTGTGTTGTATTCTGGTGAGCCTGTGCTCGATCCTGAGCGTAAAGTGTATCTGCCGAAGATCACTAT
CCGTAAACATGGTATCGATACACACTACCTGTTCAGCTACGACTTCTTCCAATCAACCGACTACCAACGCATTGGTAAGT
TAGGTGCGGCGCTTGAAGGCCTGATTGAAGTCGGTGGTTATGTGCAACGTGGCGACCGTGTGAAGGAAGTGGGTAGCTTC
ATTGAAGCGTTAGATTGGACCATCGGCGAAGCCAAACGTGGCCTCTACATCCAACGCTATAAAGGATTGGGTGAGATGAA
CCCTGAGCAATTGTGGGAAACCACAATGGATCCTGAATCTCGCCGCATGCTGCAAGTGACGATTGATGATGCCGTTGGTG
CGGATCAGCTGTTTACTTGTTTGATGGGCGACCAAGTTGAACCACGCCGTGACTTTATCGAAGCCAACGCGCTGAACGTG
GCTAACTTAGATGTTTAA

Upstream 100 bases:

>100_bases
CCTGCAGCAATAGTCGATTCGTTACACATGCCTCCCAGTAGGATGTTCCATGTGGAACAGGGACGTGTAACGGTAATTGA
ATAACCATTGAGAGAATAAT

Downstream 100 bases:

>100_bases
CATTTAGACGTTTAACGATCTATATCGTTAGTGATTGATAAAAAAGGGAAAGCTTAGCTTTCCCTTTTTGTTTGGTAGAA
AATTATCTATCAACACCAGC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDITITIHVDGSVSVRDDGRGIP
VAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGD
ATKTGTEIRFWPSEETFSDVEFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDV
FHFMQERDDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMENEGYNKKGKTNATGDDAREGL
TAVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLNDYLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALD
LGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKKGKQEQYLKDEAALTQYLTTQ
ALDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQRYPTQITNRMLYHPMINNEMLADEGKMKECIDAFIHELVE
LENSGVLYSGEPVLDPERKVYLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSF
IEALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTCLMGDQVEPRRDFIEANALNV
ANLDV

Sequences:

>Translated_805_residues
MSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDITITIHVDGSVSVRDDGRGIP
VAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGD
ATKTGTEIRFWPSEETFSDVEFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDV
FHFMQERDDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMENEGYNKKGKTNATGDDAREGL
TAVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLNDYLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALD
LGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKKGKQEQYLKDEAALTQYLTTQ
ALDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQRYPTQITNRMLYHPMINNEMLADEGKMKECIDAFIHELVE
LENSGVLYSGEPVLDPERKVYLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSF
IEALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTCLMGDQVEPRRDFIEANALNV
ANLDV
>Mature_804_residues
SENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDITITIHVDGSVSVRDDGRGIPV
AIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGDA
TKTGTEIRFWPSEETFSDVEFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDVF
HFMQERDDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMENEGYNKKGKTNATGDDAREGLT
AVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLNDYLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALDL
GGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKKGKQEQYLKDEAALTQYLTTQA
LDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQRYPTQITNRMLYHPMINNEMLADEGKMKECIDAFIHELVEL
ENSGVLYSGEPVLDPERKVYLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSFI
EALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTCLMGDQVEPRRDFIEANALNVA
NLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=550, Percent_Identity=25.0909090909091, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI19913406, Length=550, Percent_Identity=24, Blast_Score=114, Evalue=5e-25,
Organism=Escherichia coli, GI48994957, Length=803, Percent_Identity=70.8592777085928, Blast_Score=1184, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=548, Percent_Identity=40.1459854014599, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535065, Length=538, Percent_Identity=26.2081784386617, Blast_Score=132, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=507, Percent_Identity=23.6686390532544, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.9869281045752, Blast_Score=70, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=582, Percent_Identity=25.4295532646048, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17136538, Length=535, Percent_Identity=26.7289719626168, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89816; Mature: 89685

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDI
CCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
TITIHVDGSVSVRDDGRGIPVAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEEEECCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGDATKTGTEIRFWPSEETFSDV
HHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHH
EFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDV
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHHCCCCCCCHHH
FHFMQERDDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMEN
HHHHHHCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHC
EGYNKKGKTNATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLND
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEKDPGLSE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
IYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKK
CCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCC
GKQEQYLKDEAALTQYLTTQALDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQ
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYPTQITNRMLYHPMINNEMLADEGKMKECIDAFIHELVELENSGVLYSGEPVLDPERKV
HCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEE
YLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSF
EECEEEEEECCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
IEALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTC
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LMGDQVEPRRDFIEANALNVANLDV
HHCCCCCCHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
SENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDI
CCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
TITIHVDGSVSVRDDGRGIPVAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEEEECCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGDATKTGTEIRFWPSEETFSDV
HHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHH
EFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDV
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHHCCCCCCCHHH
FHFMQERDDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMEN
HHHHHHCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHC
EGYNKKGKTNATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLND
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEKDPGLSE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
IYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKK
CCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCC
GKQEQYLKDEAALTQYLTTQALDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQ
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYPTQITNRMLYHPMINNEMLADEGKMKECIDAFIHELVELENSGVLYSGEPVLDPERKV
HCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEE
YLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSF
EECEEEEEECCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
IEALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTC
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LMGDQVEPRRDFIEANALNVANLDV
HHCCCCCCHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]